5 - Quality Control

Author

CDN team

Published

Last compiled on 7 June, 2024

Quality Control

Introduction

This notebook reviews the quality control steps recommended at the beginning and at each subsequent annotation phase in analyzing scRNA-seq. In order to denoise a sparse cell by gene matrix, we take out non-informative cell data to create a better representation of an experiment. In this notebook, we will look at UMI and gene counts, filter out cells with high mitochondrial levels, investigate potential doublets (using DoubletFinder), and compare the cell by gene distribution before and after filtering.


Key Takeaways

  • QC is the process of filtering out “bad” data which makes it a subjective process where being conservative about what to keep and well-informed about your data is the best practice.

  • QC metrics include UMI and gene counts, mitochondrial gene expression, and doublet detection.

  • When in doubt, keep it. Once you remove something, you’ll never get it back.

Why are mitochondrial genes highly expressed and found in many datasets?

There is a disproportionate number of mitochondrial-high cells because cells upregulate mitochondrial genes during stress (such as during library prep) and near death.

From Orchestrating single-cell analysis with Bioconductor:

“High proportions (of mitochondria) are indicative of poor-quality cells (Islam et al. 2014; Ilicic et al. 2016), presumably because of loss of cytoplasmic RNA from perforated cells. The reasoning is that, in the presence of modest damage, the holes in the cell membrane permit efflux of individual transcript molecules but are too small to allow mitochondria to escape, leading to a relative enrichment of mitochondrial transcripts.”

Glossary

Doublet:

A doublet is a technical event when two or more cells end up within the same droplet. These cause confusion in the gene expression distribution especially when cells of two different cell types (different expected gene expression profiles) are in the same droplet.

Heterotypic doublets vs Homotypic doublets

A heterotypic doublet is a doublet with cells from distinct gene expression profiles that are likely different cell types.

A homotypic doublet is a doublet with cells with similar gene expression profiles that are not necessarily different cell types but are suspicious because they express around double the amount that other cells do.

Resources

  1. Current best practices in single-cell RNA-seq analysis: a tutorial

  2. DoubletFinder

  3. Bioconductor: Orchestrating single-cell analysis with Bioconductor

  4. Single Cell Best Practices

  5. Demuxafy

    A software that can access most popular demulitplexing and doublet detection tools. I used it as a review and consolidation of resources.

Loading Libraries and Data

if (!requireNamespace("tidyverse", quietly = TRUE))
    install.packages('tidyverse')
if (!requireNamespace("Seurat", quietly = TRUE))
    install.packages('Seurat')
if (!requireNamespace("colorBlindness", quietly = TRUE))
    install.packages('colorBlindness')
if (!requireNamespace("RColorBrewer", quietly = TRUE))
    install.packages('RColorBrewer')
if (!requireNamespace("DoubletFinder", quietly = TRUE))
    remotes::install_github('chris-mcginnis-ucsf/DoubletFinder')
suppressPackageStartupMessages({
  library(Seurat)
  library(tidyverse)
  library(RColorBrewer)
  library(colorBlindness)
  library(DoubletFinder)
})
set.seed(687)
# Load Seurat object
se <- readRDS('../data/Covid_Flu_Seurat_Object.rds')
se
An object of class Seurat 
33234 features across 59572 samples within 1 assay 
Active assay: RNA (33234 features, 0 variable features)
 1 layer present: counts
# Set color palette
pal <- paletteMartin
names(pal) <- sort(unique(se$Celltype))

Filter out the overexpressed genes

Let’s look at the number of counts per barcode, the number of genes per barcode, and the percentage of mitochondrial genes per barcode. These distributions will help us determine the quality of the data by allowing us to see any outliers. These outliers could be dying cells with high mitochondrial counts or doublets with high gene counts.

In public datasets, use colnames() to find the processing and metadata already analyzed.

# Correct calculation of the number of metadata variables
vars <- length(colnames(se@meta.data))
# Use paste0 for concatenation without spaces
print(paste0("There are ", vars, " metadata variables in the seurat object."))
[1] "There are 49 metadata variables in the seurat object."
# Sample the first 10 metadata variables
colnames(se@meta.data)[1:10]
 [1] "orig.ident"                "nCount_RNA"                "nFeature_RNA"              "Sample ID"                 "Disease group"             "Comorbidity"               "Hospital day"              "WBC per microL"            "Neutrophil per microL (%)" "Lymphocyte per microL (%)"

1 - Number of UMIs

nCount_RNA is the number of UMIs per cell. This is a measure of the total number of transcripts detected in a cell and is a good way to visualize the library size. Cells with low nCount_RNA values may be dying cells or biologically relevant cell types with low mRNA content such as neutrophils. Cells with high nCount_RNA values may be doublets or very large cells like macrophages. Note the figure below plots UMIs on a log10 scale.

ggplot(se@meta.data, aes(x = nCount_RNA)) +
    geom_density(color = "#6abcb6", fill = "#6abcb6", alpha = 0.7) +
    scale_x_continuous(
        transform = "log10",
        labels = scales::unit_format(unit = "K", scale = 1e-3)) +
    theme_classic() +
  scale_color_manual(values = pal)

Plotting the UMIs by cell type shows that if we were to make a hard cut off at 500 UMIs, we would lose a large portion of platelets.

ggplot(se@meta.data, aes(x = nCount_RNA, fill = Celltype)) +
    geom_density(alpha = 0.7) +
  # Hard cutoff at 500 elicits loss in important cells
  geom_vline(xintercept = 500, linetype = "dashed", color = "red") +
    scale_x_continuous(
        transform = "log10",
        labels = scales::unit_format(unit = "K", scale = 1e-3)) +
    theme_classic() +
  scale_color_manual(values = pal)

2 - Number of genes

nFeature_RNA is the number of genes detected in a cell. Similarly, we want to look for cells with significantly low or high feature count. The feature count distribution gives us an idea of library complexity while UMI counts looks at library size. Note that the figure below plots genes (features) on a log10 scale.

ggplot(se@meta.data, aes(x = nFeature_RNA)) +
    geom_density(color = "#6abcb6", fill = "#6abcb6", alpha = 0.7) +
    scale_x_continuous(
        transform = "log10",
        labels = scales::unit_format(unit = "K", scale = 1e-3)) +
    theme_classic() +
  scale_color_manual(values = pal)

A plot showing incredibly high number of genes per cell is indicative of doublets or large cells. Cells on the left side of the distribution with low gene counts could be low quality cells or just RBCs or platelets.

3 - Percentage of mitochondrial genes

Notice the object already has Percent of mitochondrial genes calculated. We are going to calculate them again to show the process.

Seurat’s PercentageFeatureSet function calculates the percentage of a feature set in each cell. This function is useful for calculating the percentage of mitochondrial genes, ribosomal genes, or any other gene set of interest using regular expression (string matching) to filter these out by gene name.

# Calculate the percentage of mitochondrial genes
se <- PercentageFeatureSet(se, pattern = "^MT-", col.name = "perc.mt")
# Calculate the percentage of ribosomal genes
se <- PercentageFeatureSet(se, pattern = "^RPS|^RPL", col.name = "perc.ribo")
# Calculate the percentage of hemoglobin genes
se <- PercentageFeatureSet(se, pattern = "^HB[^(P)]", col.name = "perc.hb")
# Plot Percent of mitochondrial genes by Sample
VlnPlot(se,
  group.by = "Sample ID",
  features = "perc.mt",
  # log = TRUE,
  pt.size = 0, 
  ) + NoLegend() +
  scale_color_manual(values = pal)

# Plot Percent of ribosomal genes by Sample
VlnPlot(se,
  group.by = "Sample ID",
  features = "perc.ribo",
  # log = TRUE,
  pt.size = 0
  ) + NoLegend() +
  scale_color_manual(values = pal)

# Plot Percent of hemoglobin genes by Sample
VlnPlot(se,
  group.by = "Sample ID",
  features = "perc.hb",
  # log = TRUE,
  pt.size = 0
  ) + NoLegend() +
  scale_color_manual(values = pal)

# Plot features by Sample together
VlnPlot(se,
  group.by = "Sample ID",
  features = c("perc.mt", "perc.ribo", "perc.hb"),
  # log = TRUE,
  pt.size = 0
  ) + NoLegend() +
  scale_color_manual(values = pal)

With these distributions, something notable is going on in Flu 5. Let’s perform other quality control visualizations to see if we can find out what is happening.

# Comparison of mitochondrial and hemoglobin genes
p1 <- se@meta.data %>%
  ggplot2::ggplot(., ggplot2::aes(x = perc.mt, y = perc.hb, color = Celltype)) +
    ggplot2::geom_point() +
    ggplot2::theme_classic()
p2 <- se@meta.data %>%
  ggplot2::ggplot(., ggplot2::aes(x = perc.mt, y = perc.hb, color = donor_id)) +
    ggplot2::geom_point() +
    ggplot2::theme_classic() 
p1 | p2

Plotting the hemoglobin genes by mitochondrial genes shows that the Flu 5 has low mitochondria and high hemoglobin suggesting its sample contained a lot of blood and is not poor quality.

Feature Covariation

TKTK This style plot colors by a specified variable in order to see its prevalence across the data. Below, the distribution of mitochondrial percentage across the dataset is shown in relation to the library size.

# Percent Mitochondrial Genes
se@meta.data %>%
  ggplot2::ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = perc.mt)) +
    ggplot2::geom_point() +
    ggplot2::theme_classic() +
    ggplot2::scale_color_gradient(low = "yellow", high = "red", limits = c(0, 20))

# Percent MT Genes per sample
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = perc.mt)) +
  geom_point() +
  facet_wrap(~`Sample ID`) +
  theme_classic() +
  scale_color_gradient(low = "yellow", high = "red", limits = c(0, 20)) +
  scale_x_continuous(
        labels = scales::unit_format(unit = "K", scale = 1e-3))

The authors mention they filter out UMIs with higher than 15% mitochondrial genes. This explains the cap we see at that range above.

Below we look at teh distribution of ribosomal expression which can be helpful as they can be more prevalent in tumor cells.

# Percent Ribosomal Genes
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = perc.ribo)) +
    geom_point() +
    theme_classic() +
    scale_color_gradient(low = "yellow", high = "red")

# Percent MT Genes per sample
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = perc.ribo)) +
  geom_point() +
  facet_wrap(~`Sample ID`) +
  theme_classic() +
  scale_color_gradient(low = "yellow", high = "red") +
  scale_x_continuous(
        labels = scales::unit_format(unit = "K", scale = 1e-3))

Lastly, let’s plot the hemoglobin distribution and investigate the likely bloodier samples.

# Percent Hemoglobin Genes
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = perc.hb)) +
  geom_point() +
  theme_classic() +
  scale_color_gradient(low = "yellow", high = "red", limits = c(0, 100))

# Percent hemoglobin Genes per sample
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = perc.hb)) +
  geom_point() +
  facet_wrap(~`Sample ID`) +
  theme_classic() +
  scale_color_gradient(low = "yellow", high = "red", limits = c(0, 100)) +
  scale_x_continuous(
        labels = scales::unit_format(unit = "K", scale = 1e-3))

The odd distribution of Flu 5 makes more sense seeing its high hemoglobin content with lower cell counts. Let’s plot the cell type annotations to see how they support this.

# Plot by cell type
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = Celltype)) +
  geom_point() +
  theme_classic() + 
  scale_color_manual(values = pal)

# Percent MT Genes per sample
se@meta.data %>%
  ggplot(., ggplot2::aes(x = nCount_RNA, y = nFeature_RNA, color = Celltype)) +
  geom_point() +
  facet_wrap(~Celltype) +
  theme_classic() +
  scale_color_manual(values = pal)

After looking at the cell type distribution, the hemoglobin concentration makes sense as it corresponds to erythocytes which are high in hemoglobin.

Add Batch Information

Preprocessed Seurat object’s will often contain batch and sample informatio as well as how doublets were calculated. From the paper for this dataset, the batch information is found in a supplementary table and the authors claimed most doublets were classified as “Uncategorized”. Because they did not include the doublet information in the metadata, we will add it in this section.

Packages to find doublets in both R and Python run one batch at a time as doublets are only a source of error at the 10X run level. We imported the batch information so we could subset the larger object and filter out doublets per batch.

# Make a data frame and extract barcode and Sample ID metadata from se obj
info <- data.frame(Barcode = colnames(se), "Sample ID" = se@meta.data$'Sample ID')
colnames(info) <- gsub("\\.", " ", colnames(info))

# Load in batch information
batch_info <- read.csv("../data/batch_info.csv")

# Rename batch_info column names to have a space instead of "."
colnames(batch_info) <- gsub("\\.", " ", colnames(batch_info))

batch_info <- batch_info %>%
  select("Sample ID", "Experimental batch")

head(batch_info)
  Sample ID Experimental batch
1     Flu 1                  1
2     Flu 2                  1
3     Flu 3                  2
4     Flu 4                  2
5     Flu 5                  2
6    nCoV 1                  1
# merge the batch_info with the info dataframe
info <- merge(info, batch_info, by = "Sample ID")

rownames(info) <- info$Barcode
info <- info %>% select(-Barcode)

# Add batch numbers to the metadata
se <- AddMetaData(se, info)

DoubletFinder

Doublet detection and removal is sensitive because you don’t want to remove any valuable outliers but still take into consideration the noisiness of the data. There are many different packages that can be used to detect doublets. If you are familiar with Python, we use Scrublet most often. But for the sake of staying in one programming language, DoubletFinder was found to be one of the most accurate R packages. DoubletFinder uses a nearest neighbor approach to identify doublets. The package has a function called doubletFinder that takes in a Seurat object and returns a Seurat object with doublet information added in the metadata.

How it Works

TKTK DoubletFinder looks at the expression profiles of cells in a batch and generates two parameters (pK & pANN) to identify doublets. DoubletFinder combines random cell’s gene expression profiles to create “fake doublets.” The artificial doublets are inserted into the data and their expression profiles are compared to the real cells to determine which cells are real doublets. This is done by a k-nearest neighbors graph where the best pK is surveyed and calculated for each batch.

Here, you have a k-nearest neighbor graph where each cell has k most-genetically-similar neighbors. pANN is the proportion of each cell’s neighbors that are artificial. A high pANN value says that a larger proportion of the cells around a specific cell in the kNN graph are fake doublets which suggests that the cell itself is a doublet. The higher the pK, the higher the pANN needs to be to make a difference.

Cells in transitioning states or cells with mixed phenotpyes can be mistaken for doublets. It is important not to disgard cells just because they have a large doublet score (pANN value) because there might be interesting biological information in these cells.

DoubletFinder allows you to set a threshold and will give each cell a categorical result of ‘Singlet’ or ‘Doublet’ but we suggest looking at the raw pANN values to understand the distribution of these cells and make threshold adjustments accordingly.

Parameters

pK parameter = the expected proportion of doublets in our dataset. This metric determines the accuracy of the model. The higher the pK, the more stringent the model is in classifying doublets. The lower the pK, the more lenient the model is in classifying doublets. pK is the PC neighborhood size that is used to compute pANN, and it is expressed as a proportion of the merged real-artificial data.

pN parameter = default number of doublets we expect to find (default = 0.25). pN functions as the threshold.

pANN = proportion of artificial nearest neighbors or the “doublet score”.

Note: DoubletFinder is only sensitive to heterotypic doublets (transcriptionally-distinct doublets) so the developer suggests using a cell-type annotations (here: batch_seurat_object@meta.data$seurat_clusters) to model the expected proportion of homotypic (transcriptionally-similar) doublets.

library(DoubletFinder)
exp_btch <- unique(se@meta.data$'Experimental batch')
# Run doubletFinder
process_batch <- function(batch) {
  print(batch)
  
  # 0 - correct for one of the batches


  # 1 - Subset Seurat object by batch
  batch_seurat_object <- subset(se, cells = which(se@meta.data$'Experimental batch' == batch))

  # 2 - Process count data of that subseted Seurat object
  batch_seurat_object <- batch_seurat_object %>%
    NormalizeData() %>%
    FindVariableFeatures() %>%
    ScaleData() %>%
    RunPCA() %>%
    FindNeighbors() %>%
    FindClusters()

  # 3 - nExp defines the expected pANN threshold used to make final doublet-decisions

  annotations <- batch_seurat_object@meta.data$seurat_clusters
  homotypic.prop <- modelHomotypic(annotations)
  nExp_poi <- round(0.075*nrow(se@meta.data))  # Assuming 7.5% doublet formation rate
  nExp_poi.adj <- round(nExp_poi*(1-homotypic.prop))

  # 6 - Run doubletFinder
  batch_seurat_object <- doubletFinder(
    batch_seurat_object,
    PCs = 1:20,
    pN = 0.1,
    pK = 50 / ncol(batch_seurat_object), # set a k to aproximately 50 cells per neighborhood for pANN
    nExp = nExp_poi.adj,
    reuse.pANN = FALSE, # If TRUE, pANN will be reused from a previous run
    sct = FALSE) # If TRUE, sctransform will be used for preprocessing

  # 7 - Format doublet information for return dataframe
  pANN <- colnames(batch_seurat_object@meta.data) %>%
    keep(grepl('^pANN*', colnames(batch_seurat_object@meta.data))) # pANN_0.25_0.3_1000
  DF_class <- colnames(batch_seurat_object@meta.data) %>%
    keep(grepl('^DF.classifications*', colnames(batch_seurat_object@meta.data))) # DF.classifications_0.25_0.3_1000
  ## Extract values from pANN variable name
  params <- gsub("^pANN_", "", pANN)  # Remove "pANN_"
  params <- strsplit(params, "_")[[1]]  # Split by "_"
  pN <- params[1]  # Extract "0.1"
  pK <- params[2]  # Extract "0.1"
  doublet_run <- params[3]  # Extract "1000"

  ## Create new columns "pN", "pK", and "doublet_run"
  colnames(batch_seurat_object@meta.data)[colnames(batch_seurat_object@meta.data) == pANN] <- "pANN"
  colnames(batch_seurat_object@meta.data)[colnames(batch_seurat_object@meta.data) == DF_class] <- "DF_class"
  batch_seurat_object@meta.data$pN <- pN
  batch_seurat_object@meta.data$pK <- pK
  batch_seurat_object@meta.data$doublet_run <- doublet_run

  # 8 - Return DF of doublet information here
  df <- data.frame(
    Barcode = colnames(batch_seurat_object),
    batch = batch_seurat_object@meta.data$'Experimental batch',
    pANN = batch_seurat_object@meta.data$'pANN',
    DF_class = batch_seurat_object@meta.data$'DF_class',
    doublet_run = batch_seurat_object@meta.data$doublet_run,
    pK = batch_seurat_object@meta.data$pK,
    pN = batch_seurat_object@meta.data$pN
    )
  rownames(df) <- df$Barcode
  return(df)

}

processed_doublets <- lapply(exp_btch, process_batch)
[1] 3
Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 18752
Number of edges: 616465

Running Louvain algorithm...
Maximum modularity in 10 random starts: 0.9052
Number of communities: 19
Elapsed time: 2 seconds
[1] "Creating 2084 artificial doublets..."
[1] "Creating Seurat object..."
[1] "Normalizing Seurat object..."
[1] "Finding variable genes..."
[1] "Scaling data..."
[1] "Running PCA..."
[1] "Calculating PC distance matrix..."
[1] "Computing pANN..."
[1] "Classifying doublets.."
[1] 4
Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 16507
Number of edges: 535537

Running Louvain algorithm...
Maximum modularity in 10 random starts: 0.9040
Number of communities: 22
Elapsed time: 1 seconds
[1] "Creating 1834 artificial doublets..."
[1] "Creating Seurat object..."
[1] "Normalizing Seurat object..."
[1] "Finding variable genes..."
[1] "Scaling data..."
[1] "Running PCA..."
[1] "Calculating PC distance matrix..."
[1] "Computing pANN..."
[1] "Classifying doublets.."
[1] 2
Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 8624
Number of edges: 286940

Running Louvain algorithm...
Maximum modularity in 10 random starts: 0.8967
Number of communities: 19
Elapsed time: 0 seconds
[1] "Creating 958 artificial doublets..."
[1] "Creating Seurat object..."
[1] "Normalizing Seurat object..."
[1] "Finding variable genes..."
[1] "Scaling data..."
[1] "Running PCA..."
[1] "Calculating PC distance matrix..."
[1] "Computing pANN..."
[1] "Classifying doublets.."
[1] 1
Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 15689
Number of edges: 524420

Running Louvain algorithm...
Maximum modularity in 10 random starts: 0.9105
Number of communities: 21
Elapsed time: 1 seconds
[1] "Creating 1743 artificial doublets..."
[1] "Creating Seurat object..."
[1] "Normalizing Seurat object..."
[1] "Finding variable genes..."
[1] "Scaling data..."
[1] "Running PCA..."
[1] "Calculating PC distance matrix..."
[1] "Computing pANN..."
[1] "Classifying doublets.."
# Add doublet information to the main seurat object
test <- bind_rows(processed_doublets)
rownames(test) <- test$Barcode
test <- test %>% select(-Barcode)
se <- AddMetaData(
    object = se, 
    metadata = test
  )
# Save Doublet information in Seurat object
saveRDS(se, '../data/Covid_Flu_Seurat_Object_DF.rds')

Load Pre-Run DF object

se <- readRDS('../data/Covid_Flu_Seurat_Object_DF.rds')

Perform Preprocessing

se <- se %>%
    NormalizeData(verbose = FALSE) %>%
    FindVariableFeatures(
        method = "vst",
        nfeatures = 3000,
        verbose = FALSE) %>%
    ScaleData(verbose = FALSE, features = VariableFeatures(.)) %>%
    RunPCA(verbose = FALSE) %>%
    FindNeighbors() %>%
    FindClusters(resolution = 0.05) %>%
    RunUMAP(dims = 1:30, verbose = FALSE)
Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 59572
Number of edges: 1894745

Running Louvain algorithm...
Maximum modularity in 10 random starts: 0.9829
Number of communities: 7
Elapsed time: 15 seconds
# Plot UMAP colored by batch
DimPlot(se, reduction = "umap", group.by = 'DF_class') +
  labs(title = "UMAP Plot Colored by Doublet Class")

Let’s look at the distribution of pANN values

# Plot UMAP by pANN
p1 <- FeaturePlot(se, reduction = "umap", features = "pANN") +
  labs(title = "UMAP Plot Colored by pANN") 
# Plot UMAP by cell type
p2 <- DimPlot(se, reduction = "umap", group.by = "Celltype") +
  labs(title = "UMAP Plot Colored by Cell Type") +
  scale_color_manual(values = pal)

p1 | p2

# DimPlot(se, reduction = "umap", group.by = "Celltype") +
#   labs(title = "UMAP Plot Colored by Batch")

Let’s see if celltypes annotated by the authors are show the pANN distribution.

if (!requireNamespace("ggridges", quietly = TRUE)) {
  install.packages("ggridges")
}

library(ggridges)

# Assuming se@meta.data is your data frame
ggplot(se@meta.data, aes(x = pANN, y = Celltype, fill = Celltype)) +
  geom_density_ridges(alpha = 0.5) +
  theme_classic() +
  scale_color_manual(values = pal)

Filter out cells

Filter out our data by 4 criteria.

  1. Any cell with less that 500 UMIs

  2. Any cell with less than 200 genes

  3. Any cell with more than 15% mitochondrial genes

se@meta.data <- se@meta.data %>%
  mutate(
    quality = if_else(nFeature_RNA > 200 & nCount_RNA > 500 & perc.mt < 15, "good quality", "bad quality"),
    quality = factor(quality, levels = c("bad quality", "good quality"))
    )
s2 <- FeaturePlot(se, reduction = "umap", features = "pANN") +
  labs(title = "UMAP Plot Colored by pANN") +
  scale_fill_gradient(limits = c(0, 1))
# Plot UMAP of good quality cells
s3 <- DimPlot(se, reduction = "umap", group.by = "quality") +
  labs(title = "UMAP Plot Colored by Cell Quality") 
s4 <- DimPlot(se, reduction = "umap", group.by = "Celltype") +
  labs(title = "UMAP Plot Colored by Cell Type") +
  scale_color_manual(values = pal)
s2 | s3 | s4

Lack of overlap between pANN dist and doublet dist is good because odds of a doublet of two poor quality cells is low.

We will keep the potential doublets in the data because they may be biologically relevant.

Differential Gene Expression comparison between good and bad quality cells

# 1 - Perform differential gene expression on se@meta.data$quality = good and bad
Idents(se) <- se$quality
mgs <- FindMarkers(
    se,
    ident.1 = "bad quality",
    ident.2 = "good quality",
    test.use = "wilcox",
    slot = "data",
    logfc.threshold = 0.25,
    min.pct = 0.25)

mgs <- mgs %>%
  rownames_to_column("gene") %>%
  mutate(
    gene_type = case_when(
      str_detect(gene, pattern = "^MT-") ~ "mt",
      str_detect(gene, pattern = "^RPS|^RPL") ~ "rb",
      str_detect(gene, pattern = "^HB[^(P)]") ~ "hb",
      TRUE ~ "other"
    )
  )
mgs
                        gene         p_val avg_log2FC pct.1 pct.2     p_val_adj gene_type
1                      RPS10  0.000000e+00 -2.0913895 0.072 0.868  0.000000e+00        rb
2                      RPS21  0.000000e+00 -2.0137987 0.115 0.902  0.000000e+00        rb
3                      RPL31  0.000000e+00 -1.6831092 0.074 0.858  0.000000e+00        rb
4                      TOMM7  0.000000e+00 -1.6797877 0.060 0.839  0.000000e+00     other
5                      RPL38  0.000000e+00 -1.7606579 0.119 0.897  0.000000e+00        rb
6                      COX7C  0.000000e+00 -1.1037346 0.096 0.856  0.000000e+00     other
7                     COMMD6  0.000000e+00 -1.7282373 0.041 0.789  0.000000e+00     other
8                      RPL23  0.000000e+00 -1.2237581 0.134 0.880  0.000000e+00        rb
9                    HNRNPA1  0.000000e+00 -1.1421124 0.103 0.843  0.000000e+00     other
10                      DDX5  0.000000e+00 -0.8216554 0.166 0.896  0.000000e+00     other
11                     RPL27  0.000000e+00 -1.2344377 0.176 0.906  0.000000e+00        rb
12                      EEF2  0.000000e+00 -0.9603728 0.120 0.850  0.000000e+00     other
13                     UQCRB  0.000000e+00 -0.7130181 0.119 0.848  0.000000e+00     other
14                     RPS20  0.000000e+00 -1.2005717 0.169 0.895  0.000000e+00        rb
15                      CD48  0.000000e+00 -0.9325543 0.094 0.817  0.000000e+00     other
16                     PNISR  0.000000e+00 -1.2632073 0.059 0.781  0.000000e+00     other
17                      BTG1  0.000000e+00 -1.3751895 0.160 0.882  0.000000e+00     other
18                      BTF3  0.000000e+00 -0.6948830 0.171 0.893  0.000000e+00     other
19                     SRSF5  0.000000e+00 -0.7764157 0.088 0.808  0.000000e+00     other
20                      CD37  0.000000e+00 -1.1098446 0.107 0.827  0.000000e+00     other
21                    EEF1B2  0.000000e+00 -1.1516194 0.155 0.875  0.000000e+00     other
22                    RPL27A  0.000000e+00 -1.2182378 0.178 0.898  0.000000e+00        rb
23                      TMA7  0.000000e+00 -0.4048852 0.160 0.880  0.000000e+00     other
24                   ATP5MC2  0.000000e+00 -0.5842837 0.133 0.851  0.000000e+00     other
25                     HINT1  0.000000e+00 -0.7213765 0.101 0.819  0.000000e+00     other
26                     RPL35  0.000000e+00 -1.1612309 0.190 0.904  0.000000e+00        rb
27                     PNRC1  0.000000e+00 -0.7560853 0.086 0.795  0.000000e+00     other
28                     RBM39  0.000000e+00 -0.4949071 0.116 0.824  0.000000e+00     other
29                   RPL36AL  0.000000e+00 -0.4620919 0.178 0.885  0.000000e+00        rb
30                     RPS29  0.000000e+00 -2.1757463 0.222 0.929  0.000000e+00        rb
31                      JUND  0.000000e+00 -1.2972509 0.206 0.912  0.000000e+00     other
32                    ATP5MG  0.000000e+00 -0.3827306 0.169 0.875  0.000000e+00     other
33                     SRRM2  0.000000e+00 -0.6633327 0.078 0.783  0.000000e+00     other
34                       SON  0.000000e+00 -0.7944571 0.070 0.774  0.000000e+00     other
35                     RPL36  0.000000e+00 -1.4542507 0.216 0.919  0.000000e+00        rb
36                     LIMD2  0.000000e+00 -0.9381949 0.073 0.775  0.000000e+00     other
37                     EIF3K  0.000000e+00 -0.3555461 0.123 0.824  0.000000e+00     other
38                     RPL22  0.000000e+00 -1.2591953 0.217 0.915  0.000000e+00        rb
39                     RPLP0  0.000000e+00 -0.8214933 0.189 0.887  0.000000e+00        rb
40                     NOP53  0.000000e+00 -1.4182918 0.054 0.752  0.000000e+00     other
41                     EIF3F  0.000000e+00 -0.8199362 0.072 0.769  0.000000e+00     other
42                     PTPRC  0.000000e+00 -0.4620036 0.166 0.862  0.000000e+00     other
43                       SF1  0.000000e+00 -0.8277718 0.079 0.774  0.000000e+00     other
44                     TXNIP  0.000000e+00 -1.1395119 0.182 0.877  0.000000e+00     other
45                      CCNI  0.000000e+00 -0.2771694 0.150 0.845  0.000000e+00     other
46                     COX6C  0.000000e+00 -0.6556729 0.081 0.775  0.000000e+00     other
47                   ZFP36L2  0.000000e+00 -1.1608645 0.111 0.803  0.000000e+00     other
48                      RBM3  0.000000e+00 -0.7421138 0.065 0.757  0.000000e+00     other
49                     SARAF  0.000000e+00 -0.7685619 0.110 0.801  0.000000e+00     other
50                   HNRNPDL  0.000000e+00 -0.6233054 0.081 0.771  0.000000e+00     other
51                      TLE5  0.000000e+00 -1.0257535 0.088 0.777  0.000000e+00     other
52                     CIRBP  0.000000e+00 -0.5279448 0.115 0.803  0.000000e+00     other
53                    RPL10A  0.000000e+00 -1.2690747 0.214 0.902  0.000000e+00        rb
54                     PSME1  0.000000e+00 -0.5093006 0.107 0.794  0.000000e+00     other
55                     RPL26  0.000000e+00 -1.8943796 0.240 0.927  0.000000e+00        rb
56                   SLC25A6  0.000000e+00 -0.4110531 0.146 0.833  0.000000e+00     other
57                     RPS25  0.000000e+00 -1.6351228 0.243 0.928  0.000000e+00        rb
58                      CD52  0.000000e+00 -1.5581675 0.094 0.778  0.000000e+00     other
59                    RPL23A  0.000000e+00 -1.7824790 0.237 0.921  0.000000e+00        rb
60                      RPS5  0.000000e+00 -1.0419682 0.214 0.895  0.000000e+00        rb
61                       FUS  0.000000e+00 -0.7198315 0.079 0.759  0.000000e+00     other
62                      NPM1  0.000000e+00 -0.5587635 0.111 0.791  0.000000e+00     other
63                     RPS26  0.000000e+00 -1.0393721 0.186 0.866  0.000000e+00        rb
64                    COX7A2  0.000000e+00 -0.5099730 0.072 0.750  0.000000e+00     other
65                    RPL37A  0.000000e+00 -1.3623921 0.246 0.924  0.000000e+00        rb
66                    PRRC2C  0.000000e+00 -0.6438354 0.074 0.747  0.000000e+00     other
67                     EIF3E  0.000000e+00 -1.0516082 0.058 0.730  0.000000e+00     other
68                      RPSA  0.000000e+00 -1.0682307 0.233 0.904  0.000000e+00        rb
69                      ELOB  0.000000e+00 -0.3128467 0.122 0.791  0.000000e+00     other
70                      KLF2  0.000000e+00 -1.3307575 0.093 0.760  0.000000e+00     other
71                     PCBP2  0.000000e+00 -0.4965579 0.096 0.763  0.000000e+00     other
72                      RPL5  0.000000e+00 -1.2687526 0.252 0.917  0.000000e+00        rb
73                    HNRNPU  0.000000e+00 -0.4367136 0.086 0.751  0.000000e+00     other
74                     SRRM1  0.000000e+00 -1.0139357 0.046 0.711  0.000000e+00     other
75                      EDF1  0.000000e+00 -0.2897948 0.090 0.753  0.000000e+00     other
76                      LSP1  0.000000e+00 -1.1335846 0.055 0.717  0.000000e+00     other
77                      SSR2  0.000000e+00 -0.5891299 0.068 0.728  0.000000e+00     other
78                     RPLP2  0.000000e+00 -1.5774281 0.277 0.936  0.000000e+00        rb
79                     EIF3H  0.000000e+00 -0.6151107 0.065 0.718  0.000000e+00     other
80                     SAP18  0.000000e+00 -0.2553228 0.081 0.734  0.000000e+00     other
81                   HNRNPA3  0.000000e+00 -0.4041266 0.080 0.732  0.000000e+00     other
82                   ARL6IP4  0.000000e+00 -0.6927608 0.057 0.707  0.000000e+00     other
83                       SET  0.000000e+00 -0.3955327 0.084 0.733  0.000000e+00     other
84                     VAMP2  0.000000e+00 -0.8901431 0.053 0.699  0.000000e+00     other
85                     ERP29  0.000000e+00 -0.3128825 0.084 0.728  0.000000e+00     other
86                     TCF25  0.000000e+00 -0.6081954 0.063 0.706  0.000000e+00     other
87                     TAPBP  0.000000e+00 -0.7588811 0.060 0.702  0.000000e+00     other
88                     EIF3G  0.000000e+00 -0.2713094 0.075 0.715  0.000000e+00     other
89                      RPL3  0.000000e+00 -1.3438559 0.280 0.918  0.000000e+00        rb
90                      CD44  0.000000e+00 -0.8352935 0.088 0.725  0.000000e+00     other
91                       NCL  0.000000e+00 -0.8738276 0.053 0.690  0.000000e+00     other
92                      CD74  0.000000e+00 -1.4339428 0.188 0.824  0.000000e+00     other
93                      SFPQ  0.000000e+00 -0.5797824 0.062 0.695  0.000000e+00     other
94                  HLA-DPB1  0.000000e+00 -1.8566252 0.065 0.698  0.000000e+00     other
95                      STK4  0.000000e+00 -0.2551091 0.086 0.716  0.000000e+00     other
96                    RPL35A  0.000000e+00 -1.2277631 0.320 0.949  0.000000e+00        rb
97                  HLA-DPA1  0.000000e+00 -1.7080824 0.070 0.699  0.000000e+00     other
98                     EIF3L  0.000000e+00 -1.3703708 0.034 0.661  0.000000e+00     other
99                     RPL21  0.000000e+00 -1.4207376 0.324 0.948  0.000000e+00        rb
100                    RPL34  0.000000e+00 -1.8007005 0.315 0.938  0.000000e+00        rb
101                  ANKRD12  0.000000e+00 -1.3745733 0.041 0.663  0.000000e+00     other
102                    RPS28  0.000000e+00 -1.6536380 0.316 0.938  0.000000e+00        rb
103                   STK17B  0.000000e+00 -0.5476391 0.062 0.682  0.000000e+00     other
104                   RPS15A  0.000000e+00 -1.7002797 0.324 0.944  0.000000e+00        rb
105                     RAC2  0.000000e+00 -0.2615227 0.089 0.708  0.000000e+00     other
106                    RPL37  0.000000e+00 -1.6525941 0.318 0.937  0.000000e+00        rb
107                    KMT2E  0.000000e+00 -0.8510909 0.049 0.666  0.000000e+00     other
108                    SRSF7  0.000000e+00 -0.6455855 0.060 0.675  0.000000e+00     other
109                    DDX17  0.000000e+00 -0.4320045 0.063 0.676  0.000000e+00     other
110                   HIGD2A  0.000000e+00 -0.4664875 0.068 0.681  0.000000e+00     other
111                   RPL36A  0.000000e+00 -1.2370431 0.037 0.649  0.000000e+00        rb
112                    EIF5A  0.000000e+00 -0.3038650 0.073 0.684  0.000000e+00     other
113                    EIF4B  0.000000e+00 -0.9200344 0.045 0.656  0.000000e+00     other
114                   SNRPD2  0.000000e+00 -1.2128888 0.033 0.643  0.000000e+00     other
115                     APRT  0.000000e+00 -0.4614036 0.062 0.670  0.000000e+00     other
116                  N4BP2L2  0.000000e+00 -1.0335641 0.039 0.647  0.000000e+00     other
117                    ACTR2  0.000000e+00 -0.4128657 0.079 0.686  0.000000e+00     other
118                     CAST  0.000000e+00 -0.3584160 0.081 0.687  0.000000e+00     other
119                    DDX3X  0.000000e+00 -0.4115116 0.067 0.673  0.000000e+00     other
120                    ICAM3  0.000000e+00 -0.7938164 0.047 0.651  0.000000e+00     other
121                  PPP2R5C  0.000000e+00 -1.1841767 0.051 0.655  0.000000e+00     other
122                  HNRNPH1  0.000000e+00 -0.3059294 0.075 0.678  0.000000e+00     other
123                     ARF6  0.000000e+00 -1.2490981 0.034 0.636  0.000000e+00     other
124                     SYF2  0.000000e+00 -0.5857649 0.054 0.655  0.000000e+00     other
125                    AHNAK  0.000000e+00 -1.0498486 0.058 0.659  0.000000e+00     other
126                     RPS8  0.000000e+00 -1.2687393 0.330 0.931  0.000000e+00        rb
127                    MZT2B  0.000000e+00 -0.6485882 0.050 0.651  0.000000e+00     other
128                    HLA-F  0.000000e+00 -0.2780481 0.083 0.683  0.000000e+00     other
129                   ATP5ME  0.000000e+00 -0.4743448 0.058 0.658  0.000000e+00     other
130                    UBXN1  0.000000e+00 -0.3168570 0.063 0.662  0.000000e+00     other
131                   AKAP13  0.000000e+00 -0.6302514 0.053 0.651  0.000000e+00     other
132                    HSPA5  0.000000e+00 -0.8979692 0.056 0.653  0.000000e+00     other
133                    RBM25  0.000000e+00 -0.7283929 0.048 0.642  0.000000e+00     other
134                    RPL39  0.000000e+00 -1.6660546 0.346 0.940  0.000000e+00        rb
135                    HSPA8  0.000000e+00 -0.5322465 0.070 0.663  0.000000e+00     other
136                   IQGAP1  0.000000e+00 -0.5063675 0.069 0.662  0.000000e+00     other
137                   ARGLU1  0.000000e+00 -0.7792858 0.042 0.632  0.000000e+00     other
138                   TOMM20  0.000000e+00 -0.8139692 0.042 0.632  0.000000e+00     other
139                      PNN  0.000000e+00 -0.9995703 0.042 0.630  0.000000e+00     other
140                    GSTK1  0.000000e+00 -0.4666511 0.063 0.650  0.000000e+00     other
141                    RPL17  0.000000e+00 -2.6524740 0.012 0.598  0.000000e+00        rb
142                    RPS23  0.000000e+00 -1.4705920 0.348 0.933  0.000000e+00        rb
143                     CD81  0.000000e+00 -1.4257581 0.034 0.619  0.000000e+00     other
144                   SERBP1  0.000000e+00 -0.3172182 0.062 0.646  0.000000e+00     other
145                   SEC61G  0.000000e+00 -0.3808750 0.056 0.640  0.000000e+00     other
146                    SPCS1  0.000000e+00 -0.2699236 0.060 0.643  0.000000e+00     other
147                   EIF4A2  0.000000e+00 -0.7089377 0.044 0.622  0.000000e+00     other
148                   HNRNPM  0.000000e+00 -0.7689365 0.037 0.611  0.000000e+00     other
149                      REL  0.000000e+00 -0.7627547 0.054 0.627  0.000000e+00     other
150                  RSL24D1  0.000000e+00 -0.8811758 0.034 0.606  0.000000e+00     other
151                    DDX24  0.000000e+00 -0.9669352 0.040 0.610  0.000000e+00     other
152                    RBM8A  0.000000e+00 -0.4489259 0.049 0.619  0.000000e+00     other
153                    RPS18  0.000000e+00 -1.3218255 0.362 0.931  0.000000e+00        rb
154                     NSA2  0.000000e+00 -1.0283320 0.032 0.600  0.000000e+00     other
155                    RPS27  0.000000e+00 -2.1449161 0.382 0.950  0.000000e+00        rb
156                   ARID4B  0.000000e+00 -0.8052063 0.041 0.608  0.000000e+00     other
157                   NDUFB2  0.000000e+00 -0.9265408 0.039 0.604  0.000000e+00     other
158                    EIF3M  0.000000e+00 -0.8617348 0.032 0.587  0.000000e+00     other
159                    KRT10  0.000000e+00 -0.9859828 0.031 0.586  0.000000e+00     other
160                    COPS9  0.000000e+00 -0.9573065 0.032 0.584  0.000000e+00     other
161                    RPL30  0.000000e+00 -1.6243878 0.395 0.944  0.000000e+00        rb
162                    IKZF1  0.000000e+00 -1.0441047 0.033 0.582  0.000000e+00     other
163                    RPS3A  0.000000e+00 -1.2008215 0.394 0.940  0.000000e+00        rb
164                    RPL32  0.000000e+00 -1.4285122 0.401 0.941  0.000000e+00        rb
165                    RPS12  0.000000e+00 -1.5897422 0.425 0.954  0.000000e+00        rb
166                   RPS27A  0.000000e+00 -1.4110549 0.436 0.952  0.000000e+00        rb
167                    RPS14  0.000000e+00 -1.1734459 0.427 0.943  0.000000e+00        rb
168                    RPL41  0.000000e+00 -1.5975458 0.497 0.964  0.000000e+00        rb
169                   MT-ND3  0.000000e+00 -1.2132691 0.511 0.971  0.000000e+00        mt
170                    RPL10  0.000000e+00 -1.3464548 0.531 0.956  0.000000e+00        rb
171                   EEF1A1  0.000000e+00 -1.4642202 0.568 0.959  0.000000e+00     other
172                    RPLP1  0.000000e+00 -1.0903025 0.592 0.965  0.000000e+00        rb
173                   MALAT1  0.000000e+00 -1.1512627 0.819 0.983  0.000000e+00     other
174                   SCAF11 2.747124e-307 -0.2982019 0.062 0.639 9.129791e-303     other
175                     BZW1 3.280426e-307 -0.3431132 0.057 0.629 1.090217e-302     other
176                      UXT 5.575060e-307 -0.4499888 0.052 0.618 1.852815e-302     other
177                    GTF3A 1.141948e-306 -0.3507745 0.056 0.625 3.795151e-302     other
178                  FAM133B 1.498386e-306 -0.6285549 0.049 0.611 4.979736e-302     other
179                  ANAPC16 4.024030e-306 -0.6133168 0.042 0.598 1.337346e-301     other
180                     DDX6 5.789723e-306 -0.9840550 0.039 0.592 1.924157e-301     other
181                    SEC62 1.479330e-305 -0.5329020 0.055 0.620 4.916406e-301     other
182                    RPS16 1.221113e-301 -1.2443450 0.290 0.926 4.058246e-297        rb
183                   BCLAF1 2.506196e-301 -0.5282367 0.051 0.609 8.329090e-297     other
184                   TUBA1A 2.561561e-301 -0.6424412 0.056 0.620 8.513091e-297     other
185                   PPP1CA 4.654029e-301 -0.3016293 0.075 0.657 1.546720e-296     other
186                    CRIP1 5.895350e-301 -1.9520390 0.020 0.548 1.959261e-296     other
187                    RPS24 9.442758e-300 -1.0484417 0.394 0.941 3.138206e-295        rb
188                     IER2 1.103480e-299 -0.3539144 0.115 0.730 3.667306e-295     other
189                    RPS19 5.483798e-299 -1.2530809 0.384 0.935 1.822485e-294        rb
190                     LSM7 6.933533e-299 -0.7678861 0.033 0.572 2.304290e-294     other
191                    PCSK7 1.331137e-297 -1.2420953 0.031 0.567 4.423901e-293     other
192                      EZR 3.185723e-297 -1.2516576 0.033 0.570 1.058743e-292     other
193                   NDUFB8 5.520588e-297 -0.5748994 0.038 0.580 1.834712e-292     other
194                  C9orf78 3.278806e-295 -0.9895570 0.034 0.568 1.089679e-290     other
195                     JUNB 7.888461e-295 -0.7098276 0.171 0.826 2.621651e-290     other
196                      EVL 1.008129e-293 -1.6742888 0.031 0.561 3.350416e-289     other
197                    SMDT1 4.528833e-293 -0.2935841 0.063 0.626 1.505112e-288     other
198                    FNBP1 4.790085e-293 -1.3105433 0.024 0.548 1.591937e-288     other
199                  TMEM258 1.264090e-292 -0.5301614 0.045 0.589 4.201075e-288     other
200                    SF3B2 3.140012e-292 -0.2751193 0.052 0.602 1.043551e-287     other
201                     KLF6 3.187219e-292 -0.3225033 0.128 0.752 1.059241e-287     other
202                     RPL9 8.085104e-291 -1.2006700 0.329 0.935 2.687003e-286        rb
203                  SELENOK 9.584873e-291 -0.6382619 0.044 0.586 3.185437e-286     other
204                   CCDC12 1.354357e-290 -0.9784557 0.025 0.548 4.501071e-286     other
205                    RSRP1 5.427234e-290 -0.5483995 0.050 0.595 1.803687e-285     other
206                     EPC1 3.284663e-289 -1.0017773 0.026 0.548 1.091625e-284     other
207                     RPL4 2.160483e-288 -0.8760192 0.203 0.898 7.180149e-284        rb
208                   ADGRE5 1.348485e-287 -0.7247066 0.051 0.596 4.481555e-283     other
209                    CXCR4 2.018172e-287 -1.1642842 0.093 0.668 6.707192e-283     other
210                    ZFP36 7.303508e-287 -0.4321995 0.112 0.711 2.427248e-282     other
211                     UFC1 1.215256e-286 -0.6422282 0.040 0.572 4.038781e-282     other
212                     RPS6 1.247723e-286 -1.1981703 0.312 0.922 4.146684e-282        rb
213                    EIF3A 3.473285e-285 -0.5391760 0.043 0.576 1.154311e-280     other
214                  ARHGEF1 8.266551e-285 -0.7008044 0.036 0.562 2.747305e-280     other
215                     RPL6 2.363272e-284 -1.0822195 0.311 0.930 7.854099e-280        rb
216                  HERPUD1 4.251738e-284 -0.6917031 0.054 0.596 1.413023e-279     other
217                  ATP5IF1 5.708401e-284 -0.3352735 0.052 0.592 1.897130e-279     other
218                    RPL11 9.417525e-283 -1.0145575 0.460 0.944 3.129820e-278        rb
219                    UQCRQ 1.078458e-282 -0.8088219 0.031 0.550 3.584147e-278     other
220                   SRSF11 1.880249e-281 -0.3343316 0.050 0.586 6.248820e-277     other
221                    RPL28 2.228633e-281 -0.9543674 0.520 0.949 7.406638e-277        rb
222                     HCST 2.458444e-281 -0.7043413 0.128 0.739 8.170394e-277     other
223                   EIF4A1 3.323343e-281 -0.6301431 0.042 0.570 1.104480e-276     other
224                    SNRPG 7.718710e-281 -1.0253187 0.025 0.536 2.565236e-276     other
225                    RPL14 1.958721e-280 -1.1099918 0.296 0.925 6.509615e-276        rb
226                  HNRNPA0 3.857330e-280 -1.3433001 0.021 0.527 1.281945e-275     other
227                     TPT1 7.270789e-280 -0.9477577 0.631 0.976 2.416374e-275     other
228                    CYTIP 6.330764e-279 -0.5981014 0.042 0.569 2.103966e-274     other
229                    DDX21 5.525617e-278 -0.7663045 0.039 0.561 1.836384e-273     other
230                     NKTR 2.073414e-277 -0.9242839 0.032 0.546 6.890783e-273     other
231                    RPL13 3.114521e-276 -1.0602713 0.521 0.950 1.035080e-271        rb
232                   PLAAT4 6.801585e-276 -1.4119962 0.032 0.545 2.260439e-271     other
233                    EEF1D 9.861789e-276 -0.5644292 0.205 0.905 3.277467e-271     other
234                     CUTA 5.782908e-275 -0.5160005 0.044 0.567 1.921892e-270     other
235                   SEC11A 9.569041e-275 -0.3116109 0.051 0.580 3.180175e-270     other
236                   ZBTB7A 1.673738e-274 -1.0723406 0.032 0.542 5.562501e-270     other
237                  MORF4L1 3.232037e-274  0.2580614 0.117 0.713 1.074135e-269     other
238                    RPS13 1.312215e-272 -1.1043385 0.347 0.932 4.361016e-268        rb
239                   RPL13A 1.694730e-272 -1.1411764 0.353 0.934 5.632267e-268        rb
240                   LUC7L3 1.634493e-271 -0.7560113 0.035 0.545 5.432073e-267     other
241                  NDUFA11 1.635089e-271 -0.2726818 0.053 0.580 5.434055e-267     other
242                  SEPTIN9 2.162287e-271 -0.4011916 0.046 0.566 7.186146e-267     other
243                     RPS7 9.515091e-271 -1.0294586 0.332 0.935 3.162245e-266        rb
244                     CDV3 1.401372e-270 -0.2562616 0.055 0.584 4.657320e-266     other
245                    TRA2B 1.735677e-270 -0.3936739 0.049 0.571 5.768350e-266     other
246                    EIF3D 5.092755e-268 -0.6465499 0.034 0.540 1.692526e-263     other
247  POLR2J3-ENSG00000285437 6.965814e-268 -0.8039291 0.031 0.533 2.315019e-263     other
248                  SELENOH 7.823100e-268 -0.5765241 0.040 0.550 2.599929e-263     other
249                      FYN 6.003050e-266 -0.9240667 0.039 0.546 1.995054e-261     other
250                    ANXA1 7.414227e-266 -0.6500831 0.127 0.720 2.464044e-261     other
251                     RPS3 7.889362e-266 -1.0793794 0.387 0.939 2.621951e-261        rb
252                   POLR2L 5.924874e-265 -0.4905120 0.047 0.560 1.969073e-260     other
253                   CLEC2B 3.528172e-264 -0.6821404 0.042 0.551 1.172553e-259     other
254                  TRMT112 1.576428e-262 -0.4404212 0.037 0.538 5.239101e-258     other
255                  ATP5MC3 4.866574e-262  0.3722032 0.095 0.654 1.617357e-257     other
256                    RWDD1 8.083054e-262 -0.7275252 0.034 0.531 2.686322e-257     other
257                   NDUFC2 8.530383e-262 -0.5235978 0.037 0.539 2.834988e-257     other
258                    RNPS1 1.236492e-261 -0.6380319 0.033 0.529 4.109356e-257     other
259                    MED10 7.107357e-261 -0.5153331 0.038 0.538 2.362059e-256     other
260                     NSD3 8.472404e-261 -1.1201971 0.025 0.513 2.815719e-256     other
261                    RUNX3 4.867870e-260 -1.7142061 0.026 0.514 1.617788e-255     other
262                     SUN2 1.327962e-259 -1.2318107 0.023 0.507 4.413348e-255     other
263                    ARL4C 1.396022e-259 -1.6419483 0.042 0.543 4.639539e-255     other
264                    PSMD7 1.984831e-259 -0.4172508 0.037 0.535 6.596389e-255     other
265                    ITGB2 2.611089e-259 -0.4814656 0.153 0.771 8.677693e-255     other
266                  ANKRD44 1.239248e-258 -1.2494822 0.021 0.503 4.118515e-254     other
267                   ARID1B 2.735684e-258 -1.1146025 0.027 0.513 9.091773e-254     other
268                   GGNBP2 2.173288e-257 -0.5104171 0.037 0.534 7.222706e-253     other
269                    RPS11 2.738400e-257 -1.0036456 0.263 0.926 9.100799e-253        rb
270                     RPS2 4.967912e-257 -0.9330091 0.434 0.939 1.651036e-252        rb
271                   NDUFA3 9.553623e-257 -0.8900735 0.029 0.515 3.175051e-252     other
272                     KLF3 1.797064e-255 -0.5178497 0.050 0.556 5.972363e-251     other
273                    FOXP1 3.562375e-255 -1.3118227 0.029 0.513 1.183920e-250     other
274                     AAK1 3.844891e-255 -1.2535342 0.022 0.500 1.277811e-250     other
275                     LSM8 1.762601e-254 -0.8752261 0.027 0.510 5.857829e-250     other
276                      FAU 4.245656e-254 -1.0014261 0.392 0.944 1.411001e-249     other
277                    RPL12 5.744456e-254 -1.0084270 0.325 0.933 1.909113e-249        rb
278                     EIF5 1.159606e-253  0.2728711 0.090 0.633 3.853835e-249     other
279                     CYLD 2.490086e-253 -0.7535527 0.030 0.513 8.275552e-249     other
280                      DDT 2.773077e-250 -0.5230150 0.039 0.527 9.216043e-246     other
281                    RPS15 3.508323e-250 -0.9830456 0.389 0.939 1.165956e-245        rb
282                   SMCHD1 4.622719e-250  0.2998636 0.089 0.628 1.536314e-245     other
283                    TAF1D 1.336525e-249 -0.9299621 0.030 0.509 4.441808e-245     other
284                   EIF2S3 3.575146e-249 -0.6145576 0.033 0.514 1.188164e-244     other
285                   MRPL54 4.593778e-249 -0.6368488 0.030 0.508 1.526696e-244     other
286                     RBMX 7.239313e-249 -1.3210375 0.016 0.483 2.405913e-244     other
287                      MIF 1.566386e-248 -1.1021344 0.023 0.496 5.205727e-244     other
288                     ST13 1.595800e-248 -0.5636828 0.034 0.516 5.303483e-244     other
289                  ATP6AP2 1.845368e-248 -0.3371790 0.044 0.535 6.132895e-244     other
290                  SEPTIN7 5.105776e-248  0.2823462 0.144 0.740 1.696853e-243     other
291                   UQCR11 8.042679e-248  0.3843044 0.148 0.747 2.672904e-243     other
292                   TMBIM4 3.106105e-247 -0.6122600 0.036 0.517 1.032283e-242     other
293                     MDM4 3.735914e-247 -1.0143773 0.027 0.500 1.241594e-242     other
294                   PIK3R1 1.324894e-246 -1.4993774 0.027 0.501 4.403151e-242     other
295                   ATP5MJ 2.291164e-246  0.2640736 0.130 0.708 7.614456e-242     other
296                   MYCBP2 1.282493e-245 -0.5980924 0.033 0.511 4.262237e-241     other
297                    H1-10 8.203591e-245 -0.4533740 0.091 0.622 2.726382e-240     other
298                  PRPF38B 1.998165e-244 -0.6949897 0.035 0.512 6.640703e-240     other
299                    SNRPF 3.193195e-243 -0.6730889 0.025 0.492 1.061226e-238     other
300                  HSP90B1 6.717080e-243  0.3185622 0.089 0.619 2.232354e-238     other
301                     BTG2 1.226933e-242 -1.0433362 0.030 0.502 4.077588e-238     other
302                   TNRC6B 2.405828e-242 -1.2425277 0.022 0.486 7.995530e-238     other
303                     GCC2 5.749835e-242 -1.1180547 0.024 0.489 1.910900e-237     other
304                  FAM107B 6.998628e-242 -0.6608858 0.034 0.509 2.325924e-237     other
305                     AKNA 1.432179e-241 -1.0183162 0.029 0.498 4.759703e-237     other
306                    SNHG8 3.992446e-241 -1.4881179 0.020 0.480 1.326849e-236     other
307                   PABPC1 9.217604e-241 -0.5164999 0.221 0.888 3.063378e-236     other
308                   RPL18A 3.134618e-240 -0.9654190 0.395 0.933 1.041759e-235        rb
309                    PFDN5 4.190251e-240 -0.5786232 0.226 0.907 1.392588e-235     other
310                     RNMT 2.121181e-239 -0.9467753 0.023 0.484 7.049533e-235     other
311                     RBIS 2.208601e-239 -1.0131724 0.023 0.484 7.340063e-235     other
312                  PRPF40A 3.329413e-239 -0.4318486 0.036 0.507 1.106497e-234     other
313                     GYPC 5.863404e-239 -0.5895859 0.041 0.518 1.948644e-234     other
314                   TMBIM6 9.030863e-239  0.3815942 0.155 0.752 3.001317e-234     other
315                    RPL24 1.476697e-238 -0.9270801 0.264 0.922 4.907656e-234        rb
316                     DPP7 2.698223e-238 -0.4523929 0.036 0.508 8.967276e-234     other
317                   SRSF10 5.977329e-238 -0.3233446 0.043 0.520 1.986505e-233     other
318                    SSBP1 6.082253e-238 -0.3824801 0.033 0.501 2.021376e-233     other
319                   RNF166 7.664536e-238 -0.7245512 0.029 0.492 2.547232e-233     other
320                    PLAC8 8.364834e-237 -0.3268967 0.072 0.577 2.779969e-232     other
321                    SRSF3 1.454736e-236  0.4312275 0.120 0.675 4.834669e-232     other
322                    COX5A 5.740009e-236  0.3000999 0.074 0.579 1.907634e-231     other
323                    GPBP1 1.154266e-235 -0.2662195 0.046 0.524 3.836086e-231     other
324                 C12orf57 2.720079e-235 -0.7135353 0.044 0.518 9.039909e-231     other
325                   SNRPB2 3.394551e-235 -0.5148932 0.031 0.493 1.128145e-230     other
326                   SMIM26 4.899268e-235 -0.9491779 0.022 0.475 1.628223e-230     other
327                  KHDRBS1 7.036777e-235 -0.4248875 0.032 0.496 2.338603e-230     other
328                    YPEL3 7.522089e-235  0.3802310 0.089 0.609 2.499891e-230     other
329                   NDUFB9 1.316700e-234  0.2922268 0.071 0.572 4.375920e-230     other
330                     KTN1 1.700787e-234 -0.5554084 0.032 0.494 5.652396e-230     other
331                   PPP1R2 1.222271e-233 -0.5211415 0.035 0.499 4.062097e-229     other
332                      ID2 2.336094e-233 -0.4734402 0.079 0.585 7.763774e-229     other
333                    IFI27 3.080149e-233  3.2951506 0.337 0.102 1.023657e-228     other
334                  WDR83OS 2.149355e-231  0.4598540 0.096 0.618 7.143168e-227     other
335                      IDS 3.464227e-231 -0.5973385 0.036 0.497 1.151301e-226     other
336                 HLA-DQB1 6.693716e-231 -1.4434445 0.042 0.510 2.224590e-226     other
337                     ELF1 1.416591e-230  0.5861591 0.089 0.604 4.707897e-226     other
338                  SELENOF 2.791561e-230 -0.5132003 0.030 0.486 9.277474e-226     other
339                     SBDS 6.551923e-230 -1.3648048 0.021 0.468 2.177466e-225     other
340                     UTRN 6.975525e-230 -1.2825737 0.020 0.464 2.318246e-225     other
341                    FOXN3 1.301822e-229 -0.5942898 0.031 0.486 4.326474e-225     other
342                   PRPF4B 3.137039e-229 -0.7335954 0.029 0.482 1.042563e-224     other
343                  SH3KBP1 1.146786e-228 -0.4207626 0.034 0.490 3.811230e-224     other
344                 LEPROTL1 1.623279e-228 -0.9377382 0.032 0.488 5.394806e-224     other
345                     PAXX 4.665856e-228 -1.2718024 0.020 0.464 1.550650e-223     other
346                      CSK 1.670132e-227 -0.5073329 0.036 0.494 5.550518e-223     other
347                    SSBP4 8.333488e-227 -0.9784009 0.020 0.462 2.769551e-222     other
348                     COPE 2.318760e-226  0.3915486 0.084 0.588 7.706168e-222     other
349                   DNAJA1 4.206689e-226 -0.3101899 0.043 0.505 1.398051e-221     other
350                   SNRPD3 5.325704e-226 -0.6500324 0.027 0.473 1.769944e-221     other
351                   VPS13C 7.853474e-226 -1.3459808 0.016 0.453 2.610023e-221     other
352                   METTL9 2.967235e-225 -0.4217209 0.048 0.512 9.861309e-221     other
353                    RPS4X 1.146698e-224 -0.8803091 0.395 0.935 3.810936e-220        rb
354                  KRTCAP2 1.344531e-224 -0.8654085 0.023 0.465 4.468416e-220     other
355                   NUCKS1 3.647243e-224 -0.3607111 0.042 0.502 1.212125e-219     other
356                   IL10RA 8.795575e-224 -0.3184868 0.044 0.505 2.923121e-219     other
357                   CCDC59 9.799649e-224 -1.2019125 0.018 0.454 3.256815e-219     other
358                   KANSL1 1.040120e-223 -0.9253738 0.022 0.461 3.456736e-219     other
359                    PEBP1 7.876501e-223 -1.0032572 0.022 0.461 2.617676e-218     other
360                     ATRX 8.642617e-223 -1.0645254 0.022 0.461 2.872287e-218     other
361                 HSP90AA1 8.756850e-223  0.4570486 0.211 0.853 2.910252e-218     other
362                   SNRPD1 2.909754e-222 -0.6675898 0.025 0.465 9.670275e-218     other
363                   EIF1AX 3.551388e-222 -0.3436486 0.034 0.483 1.180268e-217     other
364                     PKN1 9.418184e-222 -0.3381357 0.035 0.484 3.130039e-217     other
365                  ATP6V1F 1.151688e-221  0.3625653 0.093 0.600 3.827521e-217     other
366                 HLA-DRB1 6.877510e-221 -0.8840867 0.159 0.702 2.285672e-216     other
367                     CTSC 9.890767e-221 -0.7171567 0.041 0.494 3.287097e-216     other
368                   HP1BP3 1.816781e-220 -0.5794384 0.028 0.469 6.037891e-216     other
369                     RPL7 8.808993e-220 -0.6796909 0.259 0.920 2.927581e-215        rb
370                    MBNL1 4.900646e-219  0.5536442 0.119 0.648 1.628681e-214     other
371                     MIDN 1.260136e-218 -0.4518170 0.044 0.498 4.187935e-214     other
372                  DYNC1H1 1.865969e-218 -0.5124751 0.030 0.471 6.201361e-214     other
373                     BPTF 2.296487e-218 -0.7332308 0.030 0.469 7.632144e-214     other
374                    ARPC4 3.103911e-218  0.4481075 0.126 0.661 1.031554e-213     other
375                     CHD1 8.141690e-218 -0.2660287 0.041 0.490 2.705809e-213     other
376                    IL2RG 1.537462e-217 -0.8974722 0.036 0.480 5.109603e-213     other
377                    PSMB9 1.707240e-217  0.3521661 0.117 0.643 5.673840e-213     other
378                   ATP2B1 1.771700e-217 -0.7167291 0.035 0.479 5.888069e-213     other
379                 ARHGAP30 1.852988e-217 -0.3591213 0.039 0.487 6.158221e-213     other
380                    PTPN2 1.954515e-217 -0.8652981 0.024 0.458 6.495635e-213     other
381                    YWHAZ 3.303771e-217  0.4222483 0.195 0.812 1.097975e-212     other
382                    PSMB3 3.378446e-217  0.4319582 0.098 0.605 1.122793e-212     other
383                    DOCK8 4.301836e-217 -0.5325548 0.030 0.468 1.429672e-212     other
384                    DHX36 1.333201e-216 -1.1120140 0.019 0.446 4.430762e-212     other
385                       IK 1.435856e-216 -0.5479367 0.028 0.463 4.771923e-212     other
386                   SAMHD1 1.987870e-216 -0.5202482 0.062 0.530 6.606488e-212     other
387                   PSMB10 2.791357e-216 -1.3468018 0.017 0.443 9.276794e-212     other
388                     RTF1 4.212689e-216 -0.8667711 0.023 0.453 1.400045e-211     other
389                    MAGOH 1.054849e-215 -0.4594011 0.027 0.461 3.505685e-211     other
390                    PSMB6 1.140941e-215  0.2592743 0.053 0.511 3.791803e-211     other
391                    PGAM1 1.518877e-214  0.2750037 0.089 0.582 5.047834e-210     other
392                     SEM1 2.698000e-214 -0.4876421 0.031 0.467 8.966535e-210     other
393                   ANXA11 3.325674e-214  0.3960987 0.078 0.559 1.105254e-209     other
394                    FNBP4 9.797765e-214 -1.1119201 0.016 0.438 3.256189e-209     other
395                   ARPC5L 1.246185e-213 -0.4435961 0.035 0.473 4.141571e-209     other
396                    RPS17 2.096464e-213 -1.2689758 0.017 0.438 6.967388e-209        rb
397                     EML4 8.611448e-213 -0.9699258 0.023 0.450 2.861929e-208     other
398                   U2SURP 1.173263e-212 -0.6791733 0.026 0.455 3.899223e-208     other
399                   METRNL 1.302649e-212 -0.7864563 0.042 0.485 4.329224e-208     other
400                  NDUFA13 5.444116e-212 -0.9674990 0.021 0.444 1.809298e-207     other
401                    PRKCB 2.152675e-211  0.5093049 0.117 0.634 7.154200e-207     other
402                   EIF4G2 2.523632e-211  0.4488538 0.114 0.630 8.387039e-207     other
403                    PHF20 2.719498e-211 -0.9115679 0.022 0.445 9.037979e-207     other
404                 HNRNPUL1 3.049371e-211 -0.4385520 0.033 0.466 1.013428e-206     other
405                    MYADM 3.297885e-211 -0.6959121 0.047 0.494 1.096019e-206     other
406                     P4HB 7.211305e-211  0.4050334 0.086 0.570 2.396605e-206     other
407                    PAIP2 1.720426e-210  0.6117768 0.115 0.629 5.717665e-206     other
408                   ORMDL1 2.591899e-210 -0.6461050 0.027 0.454 8.613917e-206     other
409                    RPL18 5.329346e-210 -0.8608085 0.368 0.932 1.771155e-205        rb
410                   CHMP4A 1.563139e-209 -0.5845172 0.027 0.452 5.194937e-205     other
411                    UBE2B 2.246783e-209  0.3591922 0.075 0.547 7.466959e-205     other
412                   ATP5PB 2.824339e-209  0.3742745 0.071 0.539 9.386407e-205     other
413                    CAPZB 2.837594e-209  0.5073473 0.157 0.717 9.430460e-205     other
414                    RPL19 2.851089e-209 -0.8016911 0.474 0.942 9.475308e-205        rb
415                   MYL12B 3.570964e-209  0.2773069 0.214 0.846 1.186774e-204     other
416                   MRPS21 4.118660e-209 -1.0780578 0.016 0.431 1.368796e-204     other
417                    PDCD4 6.739636e-209 -0.8753202 0.026 0.450 2.239851e-204     other
418                    HMGN3 8.762755e-209 -0.7096460 0.024 0.446 2.912214e-204     other
419                     IER5 9.653771e-209 -0.7317349 0.034 0.465 3.208334e-204     other
420                   DNAJC8 1.557552e-208 -0.2743928 0.034 0.465 5.176369e-204     other
421                   TOMM22 2.584705e-208 -0.4039455 0.028 0.453 8.590010e-204     other
422                   CAPNS1 3.016575e-208  0.2674271 0.089 0.574 1.002529e-203     other
423                    ACTR3 3.679435e-208  0.3244482 0.089 0.573 1.222824e-203     other
424                   PET100 3.812600e-208 -0.3048294 0.037 0.470 1.267079e-203     other
425                     LSM3 4.030343e-208 -0.2698388 0.033 0.462 1.339444e-203     other
426                    SIVA1 1.021878e-207 -0.4834462 0.026 0.448 3.396110e-203     other
427                    MZT2A 1.043607e-207 -1.3420749 0.016 0.430 3.468324e-203     other
428                  ATP5F1C 1.720548e-207  0.3737191 0.067 0.529 5.718070e-203     other
429                    RACK1 2.091683e-207 -0.7152416 0.285 0.921 6.951498e-203     other
430                     EAPP 3.450502e-207 -1.0802730 0.018 0.433 1.146740e-202     other
431                   RAD23A 3.888085e-207 -0.7211430 0.024 0.443 1.292166e-202     other
432                   ATP5PD 9.044927e-207  0.4521761 0.077 0.548 3.005991e-202     other
433                  MICOS10 9.252291e-207 -0.6371664 0.029 0.451 3.074906e-202     other
434                    CWC15 1.100065e-206 -0.4214778 0.025 0.445 3.655956e-202     other
435                    DHRS7 1.432663e-206 -0.6684089 0.025 0.444 4.761312e-202     other
436                      TPR 2.099959e-206 -1.0085801 0.022 0.437 6.979003e-202     other
437                    CDC40 2.534628e-206 -0.8113479 0.021 0.436 8.423583e-202     other
438                    DDX46 2.601842e-206 -0.2698265 0.036 0.465 8.646962e-202     other
439                   TMED10 3.776860e-206 -0.5598514 0.029 0.450 1.255202e-201     other
440                      ATM 3.989326e-206 -1.0358642 0.024 0.442 1.325813e-201     other
441                    PA2G4 5.900522e-206 -0.6200815 0.026 0.445 1.960979e-201     other
442                  HLA-DRA 6.877288e-206 -1.3596632 0.169 0.657 2.285598e-201     other
443                    AKAP9 1.472881e-205 -0.3683826 0.034 0.460 4.894974e-201     other
444                    PSMB8 1.531712e-205  0.4841606 0.105 0.603 5.090490e-201     other
445                 TRAF3IP3 5.499859e-205 -0.8452183 0.026 0.444 1.827823e-200     other
446                    NR3C1 1.247876e-204 -0.8133148 0.023 0.439 4.147190e-200     other
447                     XRN2 2.514023e-203 -0.4638306 0.029 0.448 8.355105e-199     other
448                  SH3BGRL 6.666054e-203  0.6086113 0.139 0.669 2.215396e-198     other
449                     GNB1 8.190566e-203  0.2942335 0.068 0.524 2.722053e-198     other
450                     GNG5 4.362901e-202  0.3789016 0.131 0.651 1.449966e-197     other
451                   STK17A 4.792155e-202 -0.8651662 0.032 0.453 1.592625e-197     other
452                MAPK1IP1L 1.233287e-201 -0.3794706 0.031 0.450 4.098707e-197     other
453                    COX14 1.439776e-201 -0.3473432 0.033 0.454 4.784951e-197     other
454                 AURKAIP1 1.906658e-201  0.4252050 0.067 0.521 6.336588e-197     other
455                    STK10 5.559005e-200 -0.2642682 0.036 0.458 1.847480e-195     other
456                     RSF1 6.467096e-200 -0.4756704 0.030 0.447 2.149275e-195     other
457                   ATP1A1 1.358678e-199 -0.5843219 0.027 0.439 4.515430e-195     other
458                   MRPL20 4.260264e-199 -0.4033352 0.029 0.443 1.415856e-194     other
459                   RPS27L 6.098187e-199  0.4333933 0.066 0.515 2.026672e-194        rb
460                    WIPF1 6.456424e-199  0.3581139 0.096 0.576 2.145728e-194     other
461                   SQSTM1 4.624441e-198  0.6271914 0.133 0.651 1.536887e-193     other
462                    KDM2A 5.398447e-198 -0.6355501 0.025 0.433 1.794120e-193     other
463                   MRFAP1 5.427810e-198 -0.5855468 0.025 0.434 1.803878e-193     other
464                   UBALD2 6.166518e-198  0.6197810 0.114 0.609 2.049380e-193     other
465                     PJA2 7.579776e-198 -0.2694627 0.034 0.450 2.519063e-193     other
466                     TLE4 2.487176e-197 -0.9821536 0.020 0.423 8.265880e-193     other
467                    PYURF 5.857174e-197 -0.4549747 0.026 0.433 1.946573e-192     other
468                     BST2 6.033682e-197  0.3035071 0.071 0.522 2.005234e-192     other
469                     XBP1 1.005436e-196 -0.4466686 0.033 0.447 3.341465e-192     other
470                    ANXA6 1.032937e-196 -0.5267224 0.030 0.442 3.432862e-192     other
471                     CD69 1.336817e-196 -2.0018577 0.030 0.440 4.442778e-192     other
472                     NEMF 1.971846e-196 -0.8264641 0.023 0.427 6.553232e-192     other
473                   GOLGA4 3.050559e-195 -1.2277951 0.016 0.413 1.013823e-190     other
474                   NDUFB1 4.212811e-195 -0.3755715 0.035 0.448 1.400085e-190     other
475                     ENO1 8.540326e-195  0.4322219 0.166 0.717 2.838292e-190     other
476                   MRPL33 1.179824e-194 -1.3525820 0.014 0.406 3.921026e-190     other
477                    TAF10 3.524335e-194  0.4722499 0.080 0.536 1.171278e-189     other
478                    CDC37 9.457490e-194  0.2957379 0.063 0.503 3.143102e-189     other
479                    PRDX5 4.385418e-193  0.5696646 0.102 0.579 1.457450e-188     other
480                   UQCRC2 5.786547e-193 -0.8136812 0.019 0.414 1.923101e-188     other
481                     PCM1 7.140319e-193 -1.1221814 0.018 0.413 2.373014e-188     other
482                     MXD4 1.026270e-192 -1.0512077 0.020 0.416 3.410707e-188     other
483                     MYH9 1.543837e-192  0.4928413 0.124 0.623 5.130787e-188     other
484                    TRBC2 1.677928e-192 -2.0031629 0.022 0.418 5.576425e-188     other
485                    LMAN2 2.816410e-192 -0.2563911 0.031 0.437 9.360056e-188     other
486                    YPEL5 3.757524e-192  0.5469471 0.117 0.609 1.248776e-187     other
487                    RCSD1 4.115275e-192 -0.9302773 0.019 0.413 1.367671e-187     other
488               GADD45GIP1 1.034628e-191 -0.4358450 0.025 0.423 3.438484e-187     other
489                  TSC22D3 1.242128e-191 -0.3569397 0.246 0.846 4.128088e-187     other
490                   ZRANB2 5.725990e-191 -1.1433307 0.016 0.406 1.902976e-186     other
491                   INPP5D 1.083881e-190 -1.0196710 0.020 0.412 3.602170e-186     other
492                      MSN 1.084362e-190  0.3893917 0.162 0.702 3.603767e-186     other
493                    DDX18 1.315962e-190 -1.7810735 0.010 0.393 4.373469e-186     other
494                    RBM38 1.603995e-190 -0.2997619 0.058 0.487 5.330717e-186     other
495                    RPL7A 3.392902e-190 -0.6843246 0.396 0.934 1.127597e-185        rb
496                  SELENOT 5.442033e-190  0.3895588 0.066 0.502 1.808605e-185     other
497                      SSB 9.188539e-190 -0.2903914 0.033 0.437 3.053719e-185     other
498                     BRK1 1.649099e-189  0.6261391 0.139 0.650 5.480617e-185     other
499            CH17-189H20.1 1.899817e-189 -0.4707302 0.030 0.430 6.313853e-185     other
500                  ARL6IP5 2.312036e-189  0.6782860 0.143 0.659 7.683819e-185     other
501                    NOP10 2.798594e-189  0.3670035 0.085 0.539 9.300847e-185     other
502                     ISCU 3.438365e-189  0.5423171 0.093 0.555 1.142706e-184     other
503                    CFLAR 3.603723e-189  0.7247017 0.120 0.612 1.197661e-184     other
504                    SRP19 3.813448e-189 -0.6439283 0.020 0.412 1.267361e-184     other
505                    VDAC2 5.366446e-189  0.4841458 0.068 0.506 1.783485e-184     other
506                    STUB1 7.646034e-189  0.2717149 0.048 0.465 2.541083e-184     other
507                    STAT3 1.840971e-188  0.3662435 0.065 0.498 6.118283e-184     other
508                    RAP1A 1.910330e-188  0.5822601 0.076 0.521 6.348789e-184     other
509                 MPHOSPH8 1.950198e-188 -0.8032646 0.024 0.418 6.481287e-184     other
510                     GDI2 2.245695e-188  0.3448092 0.066 0.500 7.463344e-184     other
511                   GIMAP7 2.619158e-188 -1.1648693 0.023 0.415 8.704509e-184     other
512                    PSMA7 4.086098e-188  0.6984510 0.169 0.715 1.357974e-183     other
513                  PIK3IP1 4.676799e-188 -1.5506864 0.017 0.404 1.554287e-183     other
514                     GNG2 8.485252e-188 -1.1619138 0.016 0.401 2.819989e-183     other
515                    TMED2 1.207171e-187 -0.4507442 0.026 0.421 4.011912e-183     other
516                      JUN 2.124567e-187 -0.8552578 0.070 0.500 7.060787e-183     other
517                    H2AZ1 4.459141e-187  0.8851073 0.127 0.622 1.481951e-182     other
518                    HSPA9 5.325499e-187 -0.5312446 0.025 0.417 1.769876e-182     other
519                    CDK13 5.534901e-187 -0.9780389 0.020 0.407 1.839469e-182     other
520                   YTHDC1 7.478726e-187 -0.3586582 0.032 0.431 2.485480e-182     other
521                   RABAC1 7.479340e-187  0.8121176 0.112 0.591 2.485684e-182     other
522                    PPP4C 7.768986e-187  0.3099933 0.055 0.475 2.581945e-182     other
523                   ATRAID 9.354113e-187 -0.7934966 0.019 0.406 3.108746e-182     other
524                  HLA-DMA 1.536131e-186 -1.0107900 0.032 0.430 5.105179e-182     other
525                   ANP32A 1.656600e-186  0.5277018 0.066 0.498 5.505544e-182     other
526                    RAB7A 1.846635e-186  0.3849975 0.061 0.487 6.137107e-182     other
527                    APH1A 2.074051e-186 -0.4380841 0.025 0.418 6.892901e-182     other
528                   NDUFB4 2.574125e-186  0.7975490 0.098 0.562 8.554846e-182     other
529                  TMEM123 4.000599e-186  0.3087731 0.070 0.505 1.329559e-181     other
530                     CD47 1.016135e-185  0.4924254 0.080 0.524 3.377024e-181     other
531                    LAMP1 1.503011e-185  0.3785660 0.065 0.494 4.995106e-181     other
532                  TMEM230 1.709536e-185 -0.4561471 0.027 0.420 5.681471e-181     other
533                   CTNNB1 2.542844e-185 -0.6222189 0.028 0.421 8.450888e-181     other
534                     CLTA 2.968416e-185  0.3779174 0.064 0.492 9.865233e-181     other
535                     CBX3 3.398877e-185  0.5035484 0.064 0.492 1.129583e-180     other
536                 ARHGAP45 7.282537e-185  0.5125598 0.065 0.492 2.420278e-180     other
537                   TXNL4A 7.453964e-185  0.4127191 0.057 0.477 2.477250e-180     other
538                     GRB2 2.802551e-184  0.5439384 0.107 0.577 9.313998e-180     other
539                     CD55 3.185996e-184  0.3426422 0.074 0.511 1.058834e-179     other
540                    LENG8 1.632270e-183  0.2902742 0.052 0.466 5.424685e-179     other
541                  APBB1IP 3.303830e-183 -0.4709676 0.027 0.416 1.097995e-178     other
542                   UBE2L3 7.359819e-183  0.7373497 0.096 0.553 2.445962e-178     other
543                   RNF126 8.285303e-183 -1.3536595 0.013 0.389 2.753538e-178     other
544                     FXR1 2.873436e-182 -0.7474301 0.021 0.403 9.549576e-178     other
545                     SNX2 3.598669e-182 -0.2809955 0.034 0.428 1.195982e-177     other
546                    ITGAL 5.843020e-182 -0.7712027 0.023 0.408 1.941869e-177     other
547                   CIAO2B 6.000708e-182  0.2849367 0.046 0.452 1.994275e-177     other
548                     TPI1 8.235235e-182  0.6490918 0.147 0.657 2.736898e-177     other
549                     SMG1 9.550067e-182 -0.3370968 0.027 0.415 3.173869e-177     other
550                    RPL29 9.797707e-182 -0.7368864 0.391 0.933 3.256170e-177        rb
551                    TRA2A 1.357518e-181 -0.3080822 0.032 0.423 4.511575e-177     other
552                    SDCBP 2.487057e-181  0.3727551 0.100 0.559 8.265486e-177     other
553                     RNH1 5.740435e-181  0.3635770 0.073 0.503 1.907776e-176     other
554                  TMEM243 8.340066e-181 -1.1741046 0.013 0.386 2.771737e-176     other
555                    CMTM6 9.664424e-181  0.3086581 0.079 0.514 3.211875e-176     other
556                     IDI1 1.376808e-180 -0.4470936 0.028 0.414 4.575684e-176     other
557                    MIER1 4.117199e-180 -0.3290780 0.029 0.415 1.368310e-175     other
558                    ACAP2 4.600092e-180 -0.2672886 0.035 0.427 1.528794e-175     other
559                   SIGIRR 1.587375e-179 -0.4786209 0.026 0.409 5.275483e-175     other
560                    OSTF1 2.881626e-179  0.3541204 0.063 0.481 9.576797e-175     other
561                    SP110 5.332153e-179  0.2807357 0.052 0.459 1.772088e-174     other
562                   POU2F2 7.991335e-179 -1.2625750 0.024 0.404 2.655840e-174     other
563                    CNOT1 1.157732e-178 -0.9857158 0.017 0.390 3.847606e-174     other
564                    SNHG7 2.166403e-178 -0.3774663 0.034 0.424 7.199825e-174     other
565                 TBC1D10C 2.270068e-178 -0.9208962 0.020 0.396 7.544345e-174     other
566                    RGS19 2.420485e-178 -0.5144187 0.025 0.406 8.044239e-174     other
567                     USF2 3.190357e-178  0.4047208 0.066 0.485 1.060283e-173     other
568                      BSG 5.133184e-178  0.3597478 0.081 0.513 1.705962e-173     other
569                    KMT2A 1.649403e-177 -1.3172949 0.015 0.385 5.481627e-173     other
570                    PRDM2 1.783278e-177 -0.8534441 0.022 0.397 5.926547e-173     other
571                     RALY 7.895055e-177  0.2529745 0.049 0.451 2.623843e-172     other
572                     RTN4 1.204157e-176  0.4310644 0.091 0.533 4.001897e-172     other
573                   MGAT4A 1.374119e-176 -1.5139799 0.014 0.381 4.566748e-172     other
574                    VPS51 2.598541e-176 -0.8933510 0.016 0.386 8.635990e-172     other
575                     PHIP 4.533831e-176 -0.2612158 0.034 0.420 1.506773e-171     other
576                   IFITM1 6.233108e-176 -0.3831787 0.048 0.446 2.071511e-171     other
577                    UBA52 7.778659e-176 -0.7274783 0.349 0.948 2.585159e-171     other
578                    VPS28 1.222341e-175  0.8368680 0.114 0.578 4.062329e-171     other
579                     GMFG 1.634689e-175  0.4363073 0.226 0.823 5.432724e-171     other
580                   CNOT6L 2.742908e-175 -0.9675756 0.022 0.395 9.115780e-171     other
581                     NONO 4.347326e-175 -0.3026506 0.029 0.407 1.444790e-170     other
582                     FTH1 4.851643e-175  1.2130332 0.902 0.993 1.612395e-170     other
583                  ZFP36L1 4.925486e-175  0.3058510 0.080 0.508 1.636936e-170     other
584                    ITGA4 6.728327e-175 -1.2754466 0.013 0.376 2.236092e-170     other
585                    SYNE2 9.464381e-175 -1.9199982 0.018 0.386 3.145393e-170     other
586                  CHCHD10 1.118808e-174 -0.3262893 0.030 0.410 3.718248e-170     other
587                     CKLF 1.246354e-174 -0.2611954 0.034 0.418 4.142132e-170     other
588                     CCT4 1.950563e-174 -0.3447469 0.024 0.398 6.482501e-170     other
589                   CHMP2A 2.745075e-174  0.4503875 0.069 0.486 9.122982e-170     other
590                     GLTP 3.440944e-174 -0.4467037 0.025 0.400 1.143563e-169     other
591                    RBMS1 1.193827e-173 -0.4682144 0.027 0.403 3.967565e-169     other
592                  MICOS13 2.909469e-173 -0.6204068 0.019 0.386 9.669330e-169     other
593                 NCBP2AS2 3.142067e-173 -0.7027230 0.017 0.383 1.044235e-168     other
594                      EMB 3.657437e-173 -0.4862761 0.025 0.398 1.215513e-168     other
595                     TMF1 4.059998e-173 -1.0783738 0.015 0.379 1.349300e-168     other
596                   NDUFC1 1.194677e-172 -0.2891374 0.027 0.402 3.970389e-168     other
597                     VASP 2.085583e-172  0.2652386 0.091 0.527 6.931227e-168     other
598                    ITSN2 5.757560e-172 -0.3707766 0.028 0.401 1.913467e-167     other
599                   ZNF292 6.030804e-172 -0.3314414 0.031 0.407 2.004277e-167     other
600                  DNTTIP2 7.447086e-172 -0.5934436 0.019 0.384 2.474965e-167     other
601                      GP9 1.073444e-171  2.7316684 0.264 0.078 3.567485e-167     other
602                    DUSP2 2.978583e-171 -1.2960099 0.039 0.421 9.899022e-167     other
603                    DOCK2 4.485050e-171 -0.5220592 0.025 0.395 1.490561e-166     other
604                   CARD16 6.047028e-171  0.3409301 0.085 0.512 2.009669e-166     other
605                  B4GALT1 8.194982e-171 -0.7908291 0.021 0.386 2.723520e-166     other
606                   MRPL57 1.149077e-170 -0.4362247 0.023 0.391 3.818842e-166     other
607                    PSMA4 1.402252e-170  0.4750403 0.062 0.466 4.660245e-166     other
608                    ROMO1 2.453715e-170 -0.3533317 0.023 0.391 8.154675e-166     other
609                 ANKRD13D 2.939364e-170 -0.4088170 0.027 0.398 9.768682e-166     other
610                  BLOC1S2 3.787118e-170 -0.3823453 0.026 0.395 1.258611e-165     other
611                     ADD3 4.965296e-170  0.4333618 0.068 0.478 1.650167e-165     other
612                    ATG12 5.651819e-170 -0.4083510 0.025 0.393 1.878326e-165     other
613                    FKBP8 6.004727e-170  0.9057609 0.139 0.620 1.995611e-165     other
614                     LLPH 8.932803e-170 -1.3213923 0.011 0.366 2.968728e-165     other
615                     TTC3 1.052656e-169 -1.0421262 0.017 0.377 3.498397e-165     other
616                    PUF60 1.127759e-169 -0.4703466 0.020 0.382 3.747993e-165     other
617                   HNRNPL 1.177934e-169 -0.8203466 0.019 0.381 3.914747e-165     other
618                     XIST 1.461659e-169 -0.9439520 0.035 0.412 4.857676e-165     other
619                   ZNHIT1 3.177964e-169  0.3027430 0.048 0.436 1.056165e-164     other
620                  ANAPC11 3.357182e-169  0.3191871 0.049 0.440 1.115726e-164     other
621                    AIMP1 3.806851e-169 -0.7045897 0.019 0.380 1.265169e-164     other
622                     CD3E 7.777950e-169 -2.1997500 0.021 0.383 2.584924e-164     other
623                   NDUFS7 1.504759e-168  0.4128240 0.047 0.433 5.000917e-164     other
624                    RESF1 1.715887e-168 -0.2688566 0.032 0.405 5.702580e-164     other
625                     DBNL 2.347942e-168  0.4150365 0.065 0.469 7.803150e-164     other
626                  DNAJB14 6.017521e-168 -0.7803285 0.020 0.379 1.999863e-163     other
627                 RPS19BP1 7.589337e-168  0.4935375 0.062 0.463 2.522240e-163        rb
628                     GZMM 9.664537e-168 -2.1188792 0.017 0.374 3.211912e-163     other
629                    PSMA2 2.076471e-167 -1.0155606 0.016 0.371 6.900944e-163     other
630                  GPATCH8 2.910159e-167 -0.6864381 0.020 0.378 9.671624e-163     other
631                     TMC6 3.108179e-167 -0.5726675 0.024 0.387 1.032972e-162     other
632                   RB1CC1 4.871899e-167 -0.2759159 0.024 0.387 1.619127e-162     other
633                    BNIP2 5.281001e-167  0.4188957 0.064 0.465 1.755088e-162     other
634                   EIF2S2 7.134708e-167  0.3049367 0.048 0.433 2.371149e-162     other
635                     ETS1 8.535062e-167 -1.8562094 0.014 0.366 2.836543e-162     other
636                     GLG1 1.392252e-166 -0.4008403 0.023 0.384 4.627009e-162     other
637                     TAF7 2.599821e-166 -0.6723203 0.026 0.389 8.640245e-162     other
638                     RHOA 2.882631e-166  0.4518764 0.225 0.807 9.580137e-162     other
639                    BCL7C 5.165322e-166 -0.9244095 0.016 0.370 1.716643e-161     other
640                     IL32 6.103021e-166 -1.7839274 0.063 0.448 2.028278e-161     other
641                   HECTD1 6.656983e-166 -0.7645711 0.016 0.370 2.212382e-161     other
642                    ASH1L 7.251661e-166 -1.1388579 0.016 0.370 2.410017e-161     other
643                      C1D 1.146246e-165 -0.7334203 0.020 0.376 3.809435e-161     other
644                   DNAJB6 1.628198e-165  0.8251132 0.142 0.620 5.411153e-161     other
645                   PPHLN1 1.979036e-165 -0.5590201 0.022 0.379 6.577128e-161     other
646                  SEPTIN6 2.576128e-165  0.3883025 0.068 0.470 8.561502e-161     other
647                     RPL8 4.431470e-165 -0.6523636 0.373 0.934 1.472755e-160        rb
648                    WBP11 8.052486e-165 -0.8637661 0.016 0.368 2.676163e-160     other
649                     PHF3 9.715267e-165 -0.4650899 0.027 0.388 3.228772e-160     other
650                    PRDX1 2.128086e-164  0.6176348 0.067 0.466 7.072480e-160     other
651                   YME1L1 5.335746e-164 -0.5551307 0.022 0.377 1.773282e-159     other
652                    TTC14 5.602624e-164 -1.6317026 0.011 0.357 1.861976e-159     other
653                      AIP 1.047840e-163 -0.5632593 0.019 0.372 3.482393e-159     other
654                    MOB1A 1.285481e-163  0.7852706 0.116 0.564 4.272167e-159     other
655                    MYDGF 1.885174e-163  0.5285923 0.051 0.434 6.265187e-159     other
656                     RBM5 2.504655e-163  0.2734241 0.041 0.414 8.323969e-159     other
657             EPB41L4A-AS1 2.702240e-163 -0.9533326 0.016 0.366 8.980626e-159     other
658                  PPP1R18 3.120644e-163  0.5370474 0.082 0.495 1.037115e-158     other
659                   SNHG32 4.963495e-163 -0.6776563 0.020 0.373 1.649568e-158     other
660                 HLA-DRB5 8.080735e-163 -2.0917372 0.024 0.379 2.685551e-158     other
661                     VAPA 8.797971e-163  0.9695664 0.150 0.634 2.923918e-158     other
662                    MTCH1 9.268897e-163  0.4203804 0.051 0.434 3.080425e-158     other
663                    SORL1 1.812420e-162  0.4311155 0.054 0.438 6.023398e-158     other
664                    COX17 3.494499e-162 -0.2945676 0.029 0.389 1.161362e-157     other
665                   BCAP31 4.345268e-162  0.6664845 0.078 0.485 1.444106e-157     other
666                  BHLHE40 7.428875e-162 -1.3311665 0.016 0.364 2.468912e-157     other
667                   UBE2L6 8.724898e-162  0.3137331 0.056 0.442 2.899633e-157     other
668                    EIF2A 1.334512e-161 -1.4967388 0.009 0.349 4.435117e-157     other
669                  SH3GLB1 1.484011e-161  0.5822958 0.060 0.449 4.931962e-157     other
670                   ERGIC3 2.435641e-161 -0.9032272 0.015 0.361 8.094611e-157     other
671                     ARF1 3.437600e-161  0.8761670 0.213 0.768 1.142452e-156     other
672                    CNOT2 4.559165e-161 -0.7591651 0.019 0.368 1.515193e-156     other
673                   CLEC2D 4.688477e-161 -1.2125798 0.020 0.369 1.558168e-156     other
674                    CD247 4.736083e-161 -1.7140522 0.020 0.370 1.573990e-156     other
675                     NACA 6.576922e-161 -0.6271088 0.335 0.931 2.185774e-156     other
676                  TERF2IP 8.599613e-161  0.7798523 0.098 0.524 2.857995e-156     other
677                    CASP8 9.396470e-161 -0.7758836 0.020 0.370 3.122823e-156     other
678                    BAZ2A 1.620040e-160  0.4000158 0.048 0.424 5.384041e-156     other
679                  TMEM165 2.465893e-160 -0.3001618 0.026 0.380 8.195149e-156     other
680                   GTF2A2 3.109741e-160  0.6986116 0.067 0.460 1.033491e-155     other
681                     USP8 8.524817e-160 -0.6886547 0.020 0.368 2.833138e-155     other
682                    OXA1L 1.037485e-159 -0.4229811 0.023 0.374 3.447978e-155     other
683                    SUMO3 1.544990e-159  0.5831552 0.067 0.459 5.134620e-155     other
684                 PPP1R15A 6.816718e-159  0.4685647 0.106 0.535 2.265468e-154     other
685                 ATP6V0E1 7.218764e-159  0.7877514 0.181 0.694 2.399084e-154     other
686                  ZC3HAV1 9.981211e-159 -0.5518781 0.022 0.369 3.317156e-154     other
687                    PSMD4 1.315445e-158  0.2688283 0.037 0.399 4.371750e-154     other
688                     BRD7 2.029170e-158 -0.4640767 0.023 0.371 6.743742e-154     other
689                      LTB 2.278126e-158 -2.2138268 0.023 0.370 7.571123e-154     other
690                     CCT3 2.624823e-158 -0.2603382 0.024 0.374 8.723335e-154     other
691                   TMSB10 4.714735e-158 -0.6416826 0.545 0.946 1.566895e-153     other
692                    CAMLG 5.261455e-158 -0.3817563 0.022 0.368 1.748592e-153     other
693                     ADD1 1.208578e-157 -0.5295329 0.023 0.371 4.016588e-153     other
694                    EIF5B 2.336857e-157 -0.6704025 0.020 0.364 7.766311e-153     other
695                    STT3B 2.455380e-157 -0.3987499 0.023 0.370 8.160211e-153     other
696                    SIPA1 2.667387e-157 -1.0216295 0.014 0.352 8.864793e-153     other
697                    HMGB2 2.926992e-157  0.8011861 0.123 0.568 9.727565e-153     other
698                 HLA-DQA1 4.106661e-157 -1.9872945 0.017 0.358 1.364808e-152     other
699                    SASH3 5.062312e-157 -0.4106404 0.022 0.368 1.682409e-152     other
700                    EPS15 6.048885e-157 -0.8100302 0.017 0.357 2.010286e-152     other
701                 MAP1LC3B 1.256999e-156  0.9373400 0.134 0.589 4.177510e-152     other
702                    SSNA1 1.899944e-156  0.2686559 0.040 0.401 6.314273e-152     other
703                     TPM3 2.171434e-156  0.4393376 0.240 0.824 7.216543e-152     other
704                      TXN 2.316991e-156  0.8314398 0.124 0.569 7.700289e-152     other
705                     SSR4 1.089038e-155  0.8666309 0.223 0.781 3.619310e-151     other
706                    STRAP 1.123489e-155  0.3779335 0.055 0.429 3.733803e-151     other
707                  HLA-DMB 1.977203e-155 -1.0937989 0.020 0.360 6.571038e-151     other
708                      FBL 3.933599e-155 -0.5121649 0.020 0.359 1.307292e-150     other
709                      BBX 4.243032e-155 -0.6139006 0.023 0.366 1.410129e-150     other
710                   ZNF394 4.551191e-155 -0.3337811 0.025 0.369 1.512543e-150     other
711                     HELZ 7.147535e-155 -0.4720569 0.026 0.371 2.375412e-150     other
712                    DDOST 8.455469e-155 -0.4349333 0.021 0.361 2.810091e-150     other
713                     BUB3 8.503344e-155 -0.6554344 0.018 0.357 2.826001e-150     other
714                    COX19 8.652617e-155 -0.3210549 0.023 0.366 2.875611e-150     other
715                    ADPGK 1.011387e-154 -0.3470572 0.023 0.367 3.361244e-150     other
716                  NDUFAF8 1.013341e-154 -1.0443245 0.013 0.346 3.367736e-150     other
717                   DIAPH1 1.223465e-154  0.4651222 0.066 0.449 4.066065e-150     other
718                    BIRC6 1.356580e-154 -0.8668295 0.017 0.354 4.508459e-150     other
719                  SEPTIN2 1.521039e-154  0.4551088 0.056 0.430 5.055022e-150     other
720                      CD7 2.230574e-154 -1.1957079 0.026 0.371 7.413090e-150     other
721                     SNX3 2.416475e-154  0.8988307 0.164 0.648 8.030914e-150     other
722                     CST7 2.776702e-154 -1.6751771 0.050 0.412 9.228092e-150     other
723                   SELPLG 3.660083e-154 -0.8685049 0.016 0.351 1.216392e-149     other
724                   UBE2R2 4.017537e-154  0.4704554 0.058 0.434 1.335188e-149     other
725                     KRR1 4.290585e-154 -0.8951729 0.016 0.350 1.425933e-149     other
726                    TAGAP 4.454429e-154 -0.6861392 0.024 0.367 1.480385e-149     other
727                    PSMB4 8.232642e-154  0.5174870 0.044 0.406 2.736036e-149     other
728                   NFKBIA 2.618726e-153  0.3411361 0.253 0.801 8.703073e-149     other
729                    RBM23 2.786037e-153 -0.5970044 0.019 0.355 9.259114e-149     other
730                     RORA 4.887571e-153 -1.5815143 0.015 0.348 1.624335e-148     other
731                     CCZ1 5.004009e-153 -1.2237358 0.012 0.342 1.663032e-148     other
732                  ARHGAP9 5.984137e-153 -0.9878414 0.016 0.350 1.988768e-148     other
733                     TANK 8.386936e-153  0.3492736 0.036 0.389 2.787314e-148     other
734                    RBBP4 2.150491e-152 -0.3006790 0.025 0.366 7.146943e-148     other
735                  PPP4R3A 3.299485e-152 -0.5074123 0.022 0.360 1.096551e-147     other
736                   SHISA5 3.398025e-152  0.2640110 0.039 0.392 1.129299e-147     other
737                   DAZAP1 4.363743e-152 -0.5473438 0.018 0.353 1.450246e-147     other
738                   NDUFS8 4.949280e-152  0.5766712 0.050 0.414 1.644844e-147     other
739                     DAD1 7.603562e-152  1.0470915 0.115 0.543 2.526968e-147     other
740                     LSM5 2.031672e-151 -1.0797440 0.011 0.338 6.752058e-147     other
741                   SS18L2 2.456289e-151 -2.5638444 0.004 0.324 8.163231e-147     other
742                     GLRX 2.924589e-151  0.4645018 0.074 0.459 9.719579e-147     other
743                      SPN 3.018054e-151 -1.8560886 0.009 0.333 1.003020e-146     other
744                     RHOH 3.022084e-151 -2.1994554 0.009 0.333 1.004359e-146     other
745                    CAPN2 3.224610e-151  0.6491176 0.077 0.467 1.071667e-146     other
746                    PSMA5 6.294197e-151  0.3914659 0.037 0.388 2.091813e-146     other
747                    EMC10 6.373672e-151 -0.7437134 0.018 0.351 2.118226e-146     other
748                    ADRM1 1.188796e-150  0.3674179 0.041 0.394 3.950845e-146     other
749                    SIRT7 1.335833e-150 -0.4075345 0.022 0.357 4.439508e-146     other
750                     TUBB 1.883172e-150  0.5776172 0.062 0.434 6.258534e-146     other
751                   NDUFS5 1.891218e-150  1.0521810 0.168 0.652 6.285274e-146     other
752                    YIPF4 2.083503e-150 -0.4424265 0.021 0.355 6.924315e-146     other
753                   PPP1R7 2.257327e-150 -0.4383602 0.020 0.354 7.502002e-146     other
754                   SLC3A2 3.593285e-150  0.4812219 0.049 0.410 1.194192e-145     other
755                   NFE2L2 7.029536e-150  0.2725298 0.051 0.412 2.336196e-145     other
756                     EMC6 1.012658e-149  0.2893510 0.036 0.384 3.365466e-145     other
757                     NME3 1.304851e-149 -0.6091408 0.018 0.347 4.336542e-145     other
758                     ATF4 2.292389e-149  0.8418699 0.111 0.530 7.618526e-145     other
759                  CSNK1G2 4.106455e-149 -0.3715034 0.023 0.358 1.364739e-144     other
760                    ANXA2 1.324751e-148  0.4205926 0.122 0.553 4.402676e-144     other
761                    RBM33 1.512577e-148 -0.5860868 0.018 0.347 5.026899e-144     other
762                   POLR2K 1.910923e-148 -0.5871508 0.018 0.346 6.350763e-144     other
763                    MEAF6 3.089599e-148 -0.9712137 0.014 0.338 1.026797e-143     other
764                     NHP2 4.521215e-148 -0.6183248 0.016 0.342 1.502581e-143     other
765                  CCDC85B 1.039190e-147  0.9616746 0.107 0.519 3.453645e-143     other
766                    PHPT1 1.354540e-147 -0.2688377 0.025 0.359 4.501678e-143     other
767                    MKNK2 2.827543e-147 -0.3609764 0.024 0.356 9.397057e-143     other
768                     PPT1 3.503583e-147 -0.6204656 0.024 0.356 1.164381e-142     other
769                    ATF6B 3.729331e-147 -0.5771349 0.018 0.344 1.239406e-142     other
770                    RPL15 6.261335e-147 -0.5650758 0.424 0.945 2.080892e-142        rb
771                   SYNGR2 6.402146e-147 -0.2694520 0.025 0.358 2.127689e-142     other
772                  ARFGAP2 1.012496e-146 -0.5259362 0.020 0.348 3.364928e-142     other
773                    CNOT7 1.190313e-146 -0.8385133 0.015 0.337 3.955886e-142     other
774                   EIF4E2 2.401411e-146 -0.4082848 0.020 0.347 7.980848e-142     other
775                    AP2M1 2.460011e-146  0.9473761 0.124 0.551 8.175602e-142     other
776                   MT-ND5 4.125082e-146 -0.2796028 0.320 0.939 1.370930e-141        mt
777                    SYAP1 4.592092e-146 -0.3755997 0.026 0.358 1.526136e-141     other
778                   METAP2 5.910146e-146 -0.5356949 0.020 0.345 1.964178e-141     other
779                  SELENOS 1.061956e-145 -0.5167991 0.019 0.344 3.529303e-141     other
780                   GIMAP4 1.176592e-145 -0.3723450 0.027 0.360 3.910286e-141     other
781                     CAP1 2.542948e-145  0.6726020 0.193 0.696 8.451234e-141     other
782                  C6orf62 3.463976e-145  0.4431306 0.049 0.401 1.151218e-140     other
783                     TECR 6.559344e-145 -0.4516908 0.022 0.348 2.179933e-140     other
784                    TMED4 1.256577e-144 -0.5239699 0.020 0.343 4.176109e-140     other
785                   PTP4A2 2.367733e-144  0.5831724 0.231 0.782 7.868923e-140     other
786                    VPS29 3.500897e-144  0.6405948 0.056 0.412 1.163488e-139     other
787                    USP34 3.910053e-144 -0.7442901 0.019 0.341 1.299467e-139     other
788                     EMC4 3.991429e-144 -1.3319376 0.010 0.322 1.326511e-139     other
789                     SSH2 4.142974e-144  0.2971484 0.039 0.381 1.376876e-139     other
790                     FRG1 5.019273e-144 -1.1581028 0.011 0.325 1.668105e-139     other
791                  C1orf56 5.226601e-144 -1.6212350 0.012 0.327 1.737008e-139     other
792                     CCT7 9.757843e-144 -0.4598972 0.018 0.339 3.242922e-139     other
793                  TSPAN14 1.006990e-143 -0.3188449 0.025 0.352 3.346630e-139     other
794                     RER1 1.150674e-143  0.3408428 0.037 0.376 3.824150e-139     other
795                      LCK 1.710438e-143 -1.6647469 0.013 0.329 5.684469e-139     other
796                    PRPF8 3.242914e-143 -0.3157652 0.023 0.347 1.077750e-138     other
797                     NKG7 4.907353e-143 -1.6599903 0.134 0.487 1.630910e-138     other
798                    NFKB1 5.780170e-143 -1.1903423 0.010 0.322 1.920982e-138     other
799                   TCIRG1 5.825371e-143  0.5993323 0.065 0.428 1.936004e-138     other
800                  CSNK2A1 6.189242e-143 -1.1803057 0.012 0.326 2.056933e-138     other
801                     UFM1 1.078852e-142 -0.4657847 0.019 0.339 3.585458e-138     other
802                   CEMIP2 1.978285e-142 -1.3630580 0.015 0.329 6.574633e-138     other
803                    BIRC3 2.147413e-142 -0.7003282 0.022 0.343 7.136713e-138     other
804                     IDH2 2.294032e-142 -0.3240120 0.023 0.346 7.623987e-138     other
805                    TMCO1 2.994114e-142 -0.4161176 0.020 0.340 9.950637e-138     other
806                      QKI 3.382550e-142  0.4697062 0.058 0.414 1.124157e-137     other
807                     CCNY 3.423162e-142  0.2528328 0.044 0.386 1.137654e-137     other
808                    LTA4H 5.479896e-142 -0.4002955 0.034 0.366 1.821189e-137     other
809                  C1orf43 7.374195e-142  0.4835597 0.047 0.392 2.450740e-137     other
810                   ARID4A 9.472039e-142 -0.4544906 0.022 0.342 3.147938e-137     other
811                     NSD1 1.256311e-141 -0.6326007 0.016 0.332 4.175224e-137     other
812                   RNF19A 1.806207e-141 -0.3744279 0.027 0.353 6.002749e-137     other
813                     CRBN 2.837978e-141  0.8879197 0.067 0.429 9.431736e-137     other
814                    WDR82 3.134777e-141 -0.6754544 0.016 0.330 1.041812e-136     other
815                    DGUOK 3.271448e-141  0.4824134 0.043 0.383 1.087233e-136     other
816                    EIF1B 4.235973e-141  1.2206048 0.134 0.563 1.407783e-136     other
817                    RAB1A 4.653501e-141  0.3037126 0.037 0.372 1.546544e-136     other
818                    LNPEP 4.681025e-141 -1.4766430 0.010 0.318 1.555692e-136     other
819                  DYNLRB1 4.879681e-141  1.0004242 0.105 0.504 1.621713e-136     other
820                     DOK2 6.833671e-141  0.5125225 0.083 0.461 2.271102e-136     other
821                   EXOSC6 1.223707e-140 -0.7085253 0.015 0.328 4.066868e-136     other
822                      TES 1.268747e-140 -0.5969708 0.018 0.333 4.216554e-136     other
823                 APOBEC3G 2.203600e-140 -1.0847900 0.015 0.326 7.323445e-136     other
824                    PARK7 3.446622e-140  1.0823357 0.186 0.674 1.145450e-135     other
825                     SDF4 8.257974e-140 -0.3171175 0.021 0.338 2.744455e-135     other
826                 TMEM167A 9.232862e-140  0.2974706 0.045 0.385 3.068449e-135     other
827                  SLC38A1 9.422607e-140 -2.0183752 0.009 0.314 3.131509e-135     other
828                  PPP2R1A 1.436149e-139 -0.3915578 0.018 0.333 4.772898e-135     other
829                   MAP3K1 1.441204e-139 -0.3965350 0.024 0.344 4.789696e-135     other
830                     CTSS 1.451605e-139 -0.5037091 0.231 0.674 4.824265e-135     other
831                    SPG21 1.583138e-139 -0.7326228 0.016 0.329 5.261402e-135     other
832                    THOC7 1.716472e-139  0.2536765 0.034 0.363 5.704523e-135     other
833                    PDCD2 2.531450e-139 -0.6825748 0.014 0.324 8.413020e-135     other
834                     HM13 3.413399e-139  0.3303219 0.042 0.378 1.134409e-134     other
835                    LARP7 4.049957e-139 -0.3755003 0.021 0.337 1.345963e-134     other
836                     USP7 4.887451e-139 -0.3441938 0.022 0.339 1.624295e-134     other
837                    PREX1 6.581047e-139  0.4805695 0.042 0.379 2.187145e-134     other
838                      CD2 8.649189e-139 -2.5497950 0.008 0.311 2.874471e-134     other
839                   NECAP2 9.023849e-139 -0.5764882 0.016 0.328 2.998986e-134     other
840                   MAP3K2 1.096278e-138  0.5520241 0.048 0.389 3.643372e-134     other
841                     URI1 1.163605e-138 -0.9145495 0.014 0.322 3.867124e-134     other
842                   RNF125 1.201462e-138 -1.9628125 0.009 0.312 3.992939e-134     other
843                 MAPKAPK2 1.465882e-138  0.3434209 0.045 0.383 4.871712e-134     other
844                     RBM6 1.715371e-138 -0.6929985 0.016 0.325 5.700863e-134     other
845                    APMAP 2.289251e-138 -1.0798904 0.018 0.330 7.608097e-134     other
846                    BCAS2 4.032070e-138 -1.2899417 0.010 0.314 1.340018e-133     other
847                 ARHGAP15 4.573402e-138 -2.4999026 0.006 0.305 1.519924e-133     other
848                     MAF1 5.200695e-138  0.7093268 0.064 0.419 1.728399e-133     other
849                   SLC2A3 7.438302e-138  0.5186709 0.083 0.456 2.472045e-133     other
850                 PAFAH1B1 8.386062e-138  0.4245209 0.041 0.373 2.787024e-133     other
851                   BNIP3L 1.630423e-137  0.4679991 0.077 0.442 5.418547e-133     other
852                     LYAR 1.793899e-137 -1.0456560 0.020 0.333 5.961844e-133     other
853                   RPS4Y1 2.133754e-137 -1.4486197 0.027 0.345 7.091317e-133        rb
854                  ATP6V0B 2.567513e-137  0.8645944 0.152 0.596 8.532873e-133     other
855                   MCRIP1 3.773054e-137 -0.2892467 0.022 0.334 1.253937e-132     other
856                   TRIM22 4.201815e-137  0.3072384 0.039 0.369 1.396431e-132     other
857                    SYNE1 4.960407e-137 -1.3785823 0.015 0.321 1.648542e-132     other
858                     SELL 5.074300e-137  0.5159130 0.082 0.452 1.686393e-132     other
859                     PRNP 5.077188e-137 -0.4173040 0.020 0.330 1.687353e-132     other
860                    APEX1 5.173010e-137 -0.2967828 0.020 0.331 1.719198e-132     other
861                     NPC2 5.569450e-137  0.2729399 0.111 0.509 1.850951e-132     other
862                     MPC2 6.776717e-137  0.3960922 0.044 0.379 2.252174e-132     other
863                   CLNS1A 6.985079e-137 -0.3337536 0.018 0.327 2.321421e-132     other
864                     CD46 7.063966e-137  0.2564607 0.038 0.367 2.347639e-132     other
865                    SKAP1 7.491427e-137 -2.1800708 0.008 0.307 2.489701e-132     other
866                     LSM6 9.777909e-137 -0.5662279 0.016 0.324 3.249590e-132     other
867             RP11-138A9.1 1.303399e-136 -1.1625804 0.016 0.323 4.331717e-132     other
868                     CHD4 1.544834e-136  0.4145998 0.041 0.370 5.134101e-132     other
869                     JPT1 1.778065e-136  0.6989467 0.056 0.400 5.909221e-132     other
870                   NDFIP1 1.958295e-136  0.7302837 0.068 0.424 6.508196e-132     other
871                    GSPT1 2.319831e-136 -1.1847259 0.013 0.316 7.709725e-132     other
872                   DNAJB1 2.747878e-136 -0.4988789 0.022 0.335 9.132297e-132     other
873              THUMPD3-AS1 3.957595e-136 -0.3661154 0.024 0.338 1.315267e-131     other
874                     ETFB 4.587452e-136  0.4159366 0.037 0.363 1.524594e-131     other
875                    PSMF1 5.620739e-136 -0.6030033 0.017 0.324 1.867996e-131     other
876                     PPCS 6.901898e-136 -0.3526756 0.021 0.331 2.293777e-131     other
877                   COMMD7 7.848381e-136  0.5879867 0.056 0.400 2.608331e-131     other
878                    PTPRE 7.997794e-136  0.6622469 0.081 0.449 2.657987e-131     other
879                   NFKBIZ 8.209095e-136  0.4398178 0.066 0.418 2.728211e-131     other
880                  POLR3GL 8.978348e-136 -0.6121567 0.019 0.328 2.983864e-131     other
881                    STAT1 1.210531e-135  0.4148848 0.051 0.389 4.023078e-131     other
882                GABARAPL2 1.369660e-135  1.0488031 0.128 0.542 4.551929e-131     other
883                     TSPO 1.883799e-135  0.3519093 0.197 0.686 6.260617e-131     other
884                     TAF9 2.446288e-135 -0.6899911 0.015 0.319 8.129995e-131     other
885                     GZMA 2.662213e-135 -1.6021941 0.040 0.364 8.847598e-131     other
886                   CYFIP2 3.091500e-135 -1.2946826 0.011 0.312 1.027429e-130     other
887                     PMF1 3.411742e-135 -0.4949077 0.016 0.321 1.133858e-130     other
888                    HDAC1 4.093853e-135 -0.5710409 0.018 0.325 1.360551e-130     other
889                    UBE3A 5.318532e-135 -1.3671679 0.010 0.307 1.767561e-130     other
890                    PCIF1 9.712403e-135 -0.7395570 0.016 0.320 3.227820e-130     other
891                 SLC9A3R1 1.158958e-134 -0.3209905 0.027 0.342 3.851682e-130     other
892                    FUBP1 1.574485e-134 -1.1494183 0.011 0.309 5.232644e-130     other
893                    PDCD5 1.846433e-134 -0.3463930 0.016 0.321 6.136434e-130     other
894                     UPP1 1.968100e-134  0.8079335 0.074 0.432 6.540782e-130     other
895                   PABPC4 2.915011e-134 -1.1852221 0.011 0.310 9.687746e-130     other
896                    IDH3B 3.532383e-134 -0.4613234 0.018 0.323 1.173952e-129     other
897                   SPOCK2 3.852497e-134 -1.8963996 0.010 0.308 1.280339e-129     other
898                   TM9SF2 4.064367e-134 -0.3303114 0.021 0.329 1.350752e-129     other
899                  PLEKHA2 5.767016e-134 -1.0772108 0.012 0.311 1.916610e-129     other
900                    SPSB3 6.261077e-134 -0.6009093 0.018 0.322 2.080806e-129     other
901                  CCDC88C 1.359195e-133 -0.4610010 0.024 0.334 4.517147e-129     other
902                   NUFIP2 2.031727e-133 -0.3340833 0.020 0.326 6.752240e-129     other
903                    SRPK2 2.151108e-133 -0.3760080 0.020 0.326 7.148992e-129     other
904                     BDP1 2.171356e-133 -1.4159367 0.010 0.305 7.216284e-129     other
905                     SETX 3.249233e-133 -0.5461041 0.020 0.325 1.079850e-128     other
906                    PRDX2 3.806896e-133 -0.3899536 0.025 0.334 1.265184e-128     other
907                  MMP24OS 4.391989e-133  0.5635300 0.061 0.405 1.459634e-128     other
908                   ATF7IP 4.456689e-133 -1.2168025 0.012 0.310 1.481136e-128     other
909                    SLIRP 4.729584e-133 -0.6488327 0.013 0.311 1.571830e-128     other
910                  THEMIS2 5.387168e-133  0.2904639 0.053 0.389 1.790371e-128     other
911                     BIN1 7.389920e-133 -0.8647194 0.015 0.314 2.455966e-128     other
912                     RFC1 9.580188e-133 -1.0726360 0.010 0.305 3.183880e-128     other
913                    TIAL1 1.001862e-132 -0.3095364 0.021 0.326 3.329590e-128     other
914                 ATP6V0D1 1.161869e-132  0.8064689 0.096 0.473 3.861355e-128     other
915                    PSMD6 1.249163e-132  0.4148030 0.038 0.359 4.151469e-128     other
916                    TNIP1 1.382067e-132  0.4660931 0.042 0.367 4.593162e-128     other
917                   SFSWAP 1.567425e-132 -0.8844251 0.011 0.307 5.209181e-128     other
918                   RNF115 1.669164e-132 -0.5589251 0.020 0.324 5.547300e-128     other
919                    PFDN1 1.700633e-132 -0.3855685 0.016 0.317 5.651885e-128     other
920                     VMP1 1.724589e-132  0.3164715 0.056 0.395 5.731498e-128     other
921                 C16orf54 1.787236e-132 -1.1745601 0.013 0.310 5.939701e-128     other
922                     PUM1 2.420456e-132 -0.4680804 0.017 0.318 8.044144e-128     other
923                   RNF149 2.709448e-132  0.8225440 0.067 0.414 9.004579e-128     other
924                   MRPL47 3.219566e-132 -0.3701939 0.016 0.316 1.069990e-127     other
925                    PSMC3 5.183684e-132 -0.2832365 0.016 0.316 1.722745e-127     other
926                     H4C3 7.166031e-132  0.5657433 0.053 0.387 2.381559e-127     other
927                 SREK1IP1 8.071490e-132 -0.8995277 0.013 0.309 2.682479e-127     other
928                     DHX9 1.127484e-131 -0.5581034 0.016 0.314 3.747082e-127     other
929                    ANXA7 1.958431e-131  0.7555568 0.063 0.405 6.508650e-127     other
930                    CYTH4 2.201188e-131  0.2737452 0.037 0.355 7.315428e-127     other
931                 C16orf72 3.417521e-131  0.4867592 0.042 0.365 1.135779e-126     other
932                     TPP1 3.917844e-131  0.3892778 0.051 0.383 1.302056e-126     other
933                  TRAPPC1 5.677588e-131  1.0773434 0.160 0.600 1.886890e-126     other
934                    GPR65 6.815346e-131 -1.0895034 0.013 0.307 2.265012e-126     other
935                    RBM26 1.076071e-130 -1.0370279 0.013 0.306 3.576215e-126     other
936                  LAMTOR2 1.515994e-130  0.3867352 0.044 0.368 5.038256e-126     other
937                    CENPC 1.785963e-130 -0.7506774 0.016 0.313 5.935469e-126     other
938                    NOSIP 1.786558e-130 -0.2513631 0.029 0.339 5.937446e-126     other
939                   MRPS34 2.009604e-130  0.3516104 0.034 0.347 6.678719e-126     other
940                     ACP1 3.678177e-130  0.8590485 0.067 0.411 1.222405e-125     other
941                    DUS1L 3.703187e-130 -0.5221647 0.016 0.312 1.230717e-125     other
942                    TRBC1 5.568007e-130 -2.8162706 0.010 0.300 1.850472e-125     other
943                     RBL2 8.782727e-130 -0.7926731 0.014 0.308 2.918852e-125     other
944                 TRAPPC10 9.224366e-130 -0.6736851 0.016 0.312 3.065626e-125     other
945                     CD99 9.741269e-130  0.6100603 0.224 0.737 3.237413e-125     other
946                    IREB2 1.224426e-129 -1.0185343 0.013 0.305 4.069258e-125     other
947                    RAB8A 1.618221e-129  0.7445319 0.074 0.423 5.377995e-125     other
948                    SAFB2 2.360806e-129 -0.6242099 0.016 0.311 7.845901e-125     other
949                    PHF11 3.612837e-129 -0.5980867 0.016 0.311 1.200690e-124     other
950                   DICER1 3.723645e-129 -0.3120152 0.022 0.321 1.237516e-124     other
951                   FAM89B 4.195145e-129  0.4391490 0.048 0.372 1.394215e-124     other
952                    TUBB1 5.249448e-129  2.7301797 0.287 0.110 1.744601e-124     other
953                 NFATC2IP 5.964141e-129 -0.9186705 0.013 0.305 1.982122e-124     other
954                   ADAM10 6.411852e-129 -0.2740441 0.022 0.322 2.130915e-124     other
955                    WHAMM 7.118998e-129 -1.0404837 0.015 0.308 2.365928e-124     other
956                    NR4A2 7.379760e-129 -1.4946193 0.015 0.308 2.452589e-124     other
957                  DNAJC15 7.964236e-129  0.9948314 0.076 0.427 2.646834e-124     other
958                    PCGF5 8.764797e-129  0.6410335 0.053 0.381 2.912893e-124     other
959                   RPL7L1 8.812370e-129 -0.6657053 0.015 0.309 2.928703e-124        rb
960                     CCNK 1.026127e-128 -0.9244116 0.011 0.300 3.410232e-124     other
961                     TOP1 1.059438e-128  0.7897019 0.065 0.405 3.520936e-124     other
962                     CTSW 1.370861e-128 -1.5681589 0.040 0.354 4.555920e-124     other
963                   LPGAT1 1.452586e-128 -0.3038065 0.020 0.318 4.827525e-124     other
964                     LCP2 1.883209e-128  0.3229594 0.037 0.351 6.258657e-124     other
965                    EPB41 1.916383e-128  0.2760805 0.048 0.371 6.368906e-124     other
966                      GLS 1.974714e-128 -0.9739949 0.011 0.299 6.562766e-124     other
967                   PYCARD 2.016463e-128  0.5195970 0.107 0.488 6.701512e-124     other
968                   ELOVL5 2.416547e-128 -0.5119933 0.016 0.309 8.031152e-124     other
969                     ETF1 4.076715e-128  0.5564828 0.039 0.354 1.354856e-123     other
970                   AGTRAP 4.781498e-128  0.6291725 0.068 0.410 1.589083e-123     other
971             RP11-138A9.2 5.458038e-128 -3.2295720 0.004 0.285 1.813924e-123     other
972                    GNG11 5.697079e-128  2.6741914 0.344 0.154 1.893367e-123     other
973                    UHMK1 6.461551e-128 -0.5200816 0.018 0.312 2.147432e-123     other
974                     SNRK 6.545409e-128 -0.5239281 0.019 0.314 2.175301e-123     other
975                    ERAP2 7.882788e-128 -0.8942456 0.014 0.304 2.619766e-123     other
976                    FBXW5 8.730815e-128  0.6638338 0.057 0.388 2.901599e-123     other
977                    COPS2 1.175504e-127 -0.4827059 0.016 0.309 3.906669e-123     other
978                   COMMD2 1.289975e-127 -1.1841236 0.010 0.295 4.287104e-123     other
979                     BIN2 1.725215e-127  0.9767336 0.182 0.640 5.733580e-123     other
980                  THUMPD1 2.093246e-127 -0.8171652 0.014 0.303 6.956693e-123     other
981                   TUBA1B 2.464072e-127  0.8923442 0.186 0.644 8.189096e-123     other
982                   ANAPC5 3.998755e-127  0.6728946 0.059 0.391 1.328946e-122     other
983                  METTL26 5.078138e-127  0.4599325 0.032 0.339 1.687668e-122     other
984                     ADA2 6.556573e-127 -0.3979303 0.026 0.326 2.179012e-122     other
985                     PRF1 7.361330e-127 -1.9775682 0.024 0.321 2.446464e-122     other
986                   TRIM56 7.818825e-127 -0.3788500 0.022 0.318 2.598508e-122     other
987                    ERBIN 8.687844e-127  0.3976449 0.044 0.362 2.887318e-122     other
988                    MKRN1 1.054077e-126  0.7551506 0.077 0.426 3.503118e-122     other
989                   NSMCE3 1.206098e-126 -1.2624181 0.012 0.299 4.008348e-122     other
990                    SPAG7 1.339238e-126 -0.3982527 0.017 0.309 4.450822e-122     other
991                     RTF2 1.587926e-126  0.5492833 0.043 0.359 5.277313e-122     other
992                 SERPINB6 1.592579e-126  0.4847636 0.051 0.374 5.292778e-122     other
993                   GNPTAB 1.614788e-126 -0.5462137 0.020 0.313 5.366586e-122     other
994                 SLC25A36 1.750822e-126 -0.6897145 0.014 0.302 5.818682e-122     other
995                  TSC22D4 3.000607e-126  0.5346729 0.048 0.368 9.972216e-122     other
996                  PSTPIP1 3.121681e-126  0.3780406 0.036 0.345 1.037459e-121     other
997                   MRPL43 3.803198e-126 -0.3779937 0.015 0.304 1.263955e-121     other
998                     HOPX 5.017657e-126 -1.7973549 0.016 0.306 1.667568e-121     other
999                   SEC11C 6.742472e-126  0.7296685 0.042 0.355 2.240793e-121     other
1000                   RAB18 6.785797e-126 -0.4974160 0.016 0.305 2.255192e-121     other
1001                    TWF2 6.790949e-126  0.7870452 0.060 0.391 2.256904e-121     other
1002                  MAPRE1 7.434656e-126  0.6513002 0.049 0.369 2.470833e-121     other
1003                 DNAJC21 9.799484e-126 -0.2973612 0.019 0.310 3.256760e-121     other
1004                  IFNGR1 1.084910e-125  0.5456070 0.060 0.390 3.605590e-121     other
1005                   SYTL1 1.099522e-125 -0.8483361 0.013 0.299 3.654153e-121     other
1006                 EIF2AK2 2.353991e-125  0.3806640 0.041 0.353 7.823253e-121     other
1007                   BIRC2 2.430535e-125 -0.6614549 0.016 0.304 8.077638e-121     other
1008                   STX10 3.015022e-125  0.4830959 0.038 0.347 1.002012e-120     other
1009                  IFITM2 4.285485e-125  0.8160042 0.244 0.770 1.424238e-120     other
1010                     MVP 4.885173e-125  0.2738521 0.035 0.340 1.623538e-120     other
1011                    BRAF 5.547880e-125 -0.7716873 0.015 0.301 1.843782e-120     other
1012                    IMP3 7.169391e-125 -0.9681257 0.011 0.293 2.382675e-120     other
1013                    ABT1 8.125846e-125 -1.2866674 0.008 0.288 2.700544e-120     other
1014                  CHCHD5 1.323986e-124 -0.6518672 0.015 0.300 4.400135e-120     other
1015                   KAT6A 1.481016e-124 -0.3953329 0.021 0.312 4.922008e-120     other
1016                 SUPT4H1 1.559670e-124  1.0415759 0.102 0.471 5.183406e-120     other
1017                   SMIM7 1.643479e-124  0.3225602 0.039 0.348 5.461938e-120     other
1018                  ZFAND6 2.356875e-124  0.6681964 0.055 0.378 7.832839e-120     other
1019                  PARP14 2.491229e-124  0.4120827 0.042 0.353 8.279349e-120     other
1020                    HEXA 2.563475e-124 -0.2668717 0.020 0.310 8.519452e-120     other
1021                  PDLIM2 2.866455e-124 -0.3550945 0.018 0.307 9.526376e-120     other
1022                     SLA 3.597015e-124  0.7564900 0.064 0.395 1.195432e-119     other
1023                    MTF2 4.817694e-124 -1.5984743 0.008 0.285 1.601112e-119     other
1024                  KDELR2 5.175018e-124  0.2795959 0.030 0.330 1.719865e-119     other
1025                   DDHD1 7.310728e-124 -0.9166261 0.013 0.295 2.429647e-119     other
1026                 RPS6KA3 7.807618e-124 -0.5469408 0.016 0.302 2.594784e-119        rb
1027                  NDUFA5 1.167133e-123  0.8454456 0.063 0.392 3.878850e-119     other
1028                 TXNDC17 1.310189e-123  0.5068697 0.038 0.344 4.354283e-119     other
1029                    TXN2 1.370445e-123  0.2783475 0.029 0.326 4.554536e-119     other
1030                    RBM4 1.382236e-123 -1.4752440 0.009 0.287 4.593722e-119     other
1031                  CDKN1B 1.475553e-123 -1.5039630 0.008 0.286 4.903852e-119     other
1032                    HPS1 1.505090e-123 -0.6290473 0.015 0.299 5.002017e-119     other
1033                    TUT4 1.640020e-123 -1.0108259 0.013 0.296 5.450442e-119     other
1034                  POLR2B 1.739110e-123 -0.6795979 0.015 0.299 5.779758e-119     other
1035                   PARP8 1.890955e-123 -0.3375246 0.023 0.314 6.284398e-119     other
1036                  IQGAP2 2.121519e-123  0.8475972 0.089 0.444 7.050655e-119     other
1037                    CCNH 2.456127e-123 -0.5481120 0.018 0.305 8.162692e-119     other
1038                  SNHG15 2.471749e-123  0.2864532 0.026 0.320 8.214611e-119     other
1039                    PTEN 3.847205e-123  0.8837176 0.057 0.380 1.278580e-118     other
1040                   CTBP1 6.429982e-123 -0.3579738 0.018 0.303 2.136940e-118     other
1041                    CHP1 7.825761e-123 -0.4909178 0.018 0.305 2.600813e-118     other
1042                   NOP58 8.130721e-123 -0.6697980 0.015 0.297 2.702164e-118     other
1043                    DNM2 8.531980e-123 -0.5190721 0.016 0.301 2.835518e-118     other
1044                   MOB3A 8.785034e-123  0.4398818 0.036 0.338 2.919618e-118     other
1045                   ATOX1 1.103884e-122  0.3877943 0.044 0.354 3.668648e-118     other
1046                   ORAI1 1.324206e-122  0.3840953 0.043 0.352 4.400867e-118     other
1047                    REST 1.473412e-122 -0.4329707 0.018 0.303 4.896737e-118     other
1048                 SAP30BP 1.512343e-122 -0.3710361 0.016 0.300 5.026119e-118     other
1049                 AKIRIN1 3.143405e-122  0.3500026 0.041 0.348 1.044679e-117     other
1050                    MDH1 4.119011e-122  0.3236641 0.030 0.325 1.368912e-117     other
1051                   CMTM3 4.393752e-122 -0.2873976 0.020 0.306 1.460220e-117     other
1052                   TSTD1 5.835866e-122 -0.6438605 0.018 0.302 1.939492e-117     other
1053                    IL16 6.044658e-122 -1.0916412 0.011 0.288 2.008882e-117     other
1054                  MMADHC 7.010887e-122 -0.6995546 0.013 0.291 2.329998e-117     other
1055               MUC20-OT1 7.320618e-122 -0.9487493 0.011 0.289 2.432934e-117     other
1056                   VMA21 1.026824e-121 -0.3997989 0.013 0.293 3.412546e-117     other
1057                    OLA1 1.207744e-121 -0.5706406 0.015 0.295 4.013818e-117     other
1058                   WDR33 1.287716e-121 -0.3167841 0.020 0.306 4.279596e-117     other
1059                C1orf162 2.098676e-121  0.5870934 0.096 0.453 6.974741e-117     other
1060                   THOC2 2.370208e-121 -0.3556454 0.022 0.308 7.877150e-117     other
1061                  TRIM44 2.574849e-121 -0.5672500 0.014 0.293 8.557255e-117     other
1062                    UPF2 2.701526e-121 -1.0264786 0.010 0.286 8.978253e-117     other
1063                  ZNF644 2.950533e-121 -0.7119592 0.014 0.293 9.805801e-117     other
1064                 ALOX5AP 4.473181e-121  0.3272110 0.062 0.385 1.486617e-116     other
1065                   AP1S2 5.931343e-121  0.2737285 0.084 0.429 1.971223e-116     other
1066                   ARL8A 6.142408e-121  0.3456984 0.032 0.327 2.041368e-116     other
1067                  SH3BP1 7.209116e-121 -0.4426139 0.015 0.294 2.395878e-116     other
1068                   ACRBP 7.982197e-121  2.7168034 0.251 0.090 2.652803e-116     other
1069                    AKT1 8.883598e-121 -0.2857671 0.017 0.298 2.952375e-116     other
1070                    NAPA 9.800556e-121  0.8375214 0.065 0.391 3.257117e-116     other
1071                   IDH3G 1.005932e-120  0.6783198 0.042 0.347 3.343115e-116     other
1072                   PRMT1 1.012988e-120 -0.5768766 0.015 0.293 3.366565e-116     other
1073                 SMARCB1 1.015776e-120 -0.9477706 0.011 0.286 3.375829e-116     other
1074                RNASEH2B 1.030822e-120 -0.5437841 0.018 0.299 3.425835e-116     other
1075                   PCF11 1.113753e-120 -0.5883907 0.016 0.295 3.701446e-116     other
1076                    FIBP 1.408424e-120  0.4056627 0.030 0.324 4.680757e-116     other
1077                   FDFT1 2.701349e-120 -0.2937803 0.019 0.301 8.977664e-116     other
1078                 MARCHF1 3.106693e-120 -0.5848845 0.024 0.311 1.032478e-115     other
1079                     BID 3.801483e-120  0.3449014 0.049 0.360 1.263385e-115     other
1080                     ZFR 4.235887e-120 -0.9958823 0.011 0.285 1.407755e-115     other
1081                    AMD1 4.309461e-120  0.4451087 0.042 0.345 1.432206e-115     other
1082                 SMARCA5 4.499202e-120 -0.6970314 0.015 0.293 1.495265e-115     other
1083                    PCNP 5.075994e-120  0.9362602 0.076 0.412 1.686956e-115     other
1084                   VPS35 6.058277e-120 -0.6056042 0.015 0.293 2.013408e-115     other
1085                  LYPLA1 6.118427e-120  0.3805141 0.034 0.329 2.033398e-115     other
1086                   STMP1 9.678236e-120  1.1196382 0.109 0.476 3.216465e-115     other
1087                RNASEH2C 1.745995e-119  0.2874391 0.030 0.321 5.802640e-115     other
1088                   MLLT6 2.083914e-119 -0.2733744 0.018 0.299 6.925679e-115     other
1089                    UFD1 2.121135e-119  0.6901208 0.047 0.354 7.049381e-115     other
1090                  MRPL51 2.536603e-119  0.5126178 0.035 0.330 8.430147e-115     other
1091                  UBE2J1 2.653767e-119  0.8236769 0.073 0.404 8.819530e-115     other
1092                APOBEC3C 3.945917e-119 -0.6092026 0.016 0.294 1.311386e-114     other
1093                   TTC17 4.555721e-119 -0.8171372 0.012 0.286 1.514048e-114     other
1094                  PITHD1 4.768234e-119 -0.5791146 0.017 0.295 1.584675e-114     other
1095                    CD3D 4.989109e-119 -1.5218219 0.023 0.308 1.658080e-114     other
1096                  MRPL11 6.666749e-119 -0.3107636 0.016 0.292 2.215627e-114     other
1097                   HIF1A 8.046489e-119  0.9336992 0.082 0.420 2.674170e-114     other
1098                  NUP214 1.028092e-118  0.2591618 0.041 0.341 3.416761e-114     other
1099                  POLR2F 1.240752e-118 -0.4910127 0.015 0.291 4.123515e-114     other
1100                    PIGT 1.462343e-118 -0.5338318 0.013 0.287 4.859952e-114     other
1101                    CD83 1.886231e-118 -0.7682874 0.027 0.313 6.268700e-114     other
1102                  KDELR1 2.317737e-118  0.4880605 0.034 0.326 7.702769e-114     other
1103                    GZMH 2.351284e-118 -2.0363102 0.034 0.323 7.814259e-114     other
1104                 HERPUD2 2.570559e-118 -1.6968799 0.008 0.275 8.542996e-114     other
1105                    LY6E 2.598897e-118  0.9516570 0.205 0.675 8.637176e-114     other
1106                    ADH5 3.311998e-118 -0.6408266 0.014 0.288 1.100710e-113     other
1107                    MPC1 3.317850e-118 -0.5509455 0.015 0.289 1.102654e-113     other
1108                    COMT 3.633321e-118  0.7711529 0.048 0.353 1.207498e-113     other
1109                   DERL2 4.565385e-118 -0.2883170 0.020 0.298 1.517260e-113     other
1110                    CYBB 5.640387e-118 -0.4074377 0.065 0.385 1.874526e-113     other
1111                   ITGB1 6.296252e-118  0.9090572 0.133 0.521 2.092497e-113     other
1112                  VPS37B 1.387485e-117 -0.3741668 0.023 0.304 4.611169e-113     other
1113                   UQCRH 1.557365e-117  0.9318744 0.250 0.775 5.175745e-113     other
1114                    CLTC 1.857592e-117  0.3668109 0.035 0.327 6.173521e-113     other
1115                    HECA 1.920269e-117 -0.3462850 0.019 0.296 6.381823e-113     other
1116                   CERT1 2.470723e-117 -0.6389241 0.015 0.287 8.211201e-113     other
1117                   WDR26 2.709863e-117  0.4287451 0.037 0.332 9.005958e-113     other
1118                  ZNF430 2.734245e-117 -1.2387503 0.010 0.279 9.086991e-113     other
1119                 CCDC186 3.032777e-117 -0.4441702 0.016 0.291 1.007913e-112     other
1120                     PF4 3.614055e-117  2.6404062 0.399 0.216 1.201095e-112     other
1121                 TNFAIP8 5.244135e-117  0.5671136 0.046 0.347 1.742836e-112     other
1122                ITGB1BP1 6.422523e-117 -1.1319519 0.010 0.278 2.134461e-112     other
1123                    NXT1 8.699931e-117 -0.4559577 0.016 0.289 2.891335e-112     other
1124                   IRAK1 8.970045e-117 -0.3751719 0.017 0.291 2.981105e-112     other
1125                   CYBC1 1.027710e-116 -0.2600462 0.020 0.298 3.415493e-112     other
1126                  OGFRL1 1.280856e-116 -0.3042121 0.020 0.297 4.256798e-112     other
1127                    KXD1 1.670007e-116 -0.4627893 0.016 0.288 5.550100e-112     other
1128                  ZNF800 1.691707e-116 -0.7960779 0.012 0.281 5.622218e-112     other
1129                 TNFSF12 2.699432e-116 -0.9288609 0.010 0.277 8.971294e-112     other
1130                     TSN 2.822110e-116 -0.7878577 0.011 0.279 9.379000e-112     other
1131                   RAP2B 3.341469e-116  0.5531107 0.053 0.360 1.110504e-111     other
1132                 MARCHF6 3.343727e-116 -0.2624042 0.023 0.303 1.111254e-111     other
1133                  MT-CO1 4.051977e-116 -0.4733824 0.866 0.993 1.346634e-111        mt
1134                   HMGA1 4.058310e-116  0.3635919 0.034 0.322 1.348739e-111     other
1135                     MFF 4.296701e-116  0.3192942 0.032 0.319 1.427966e-111     other
1136                   HADHB 4.379840e-116  0.3402835 0.029 0.313 1.455596e-111     other
1137                    SIK3 4.447480e-116 -0.8114039 0.012 0.280 1.478076e-111     other
1138                    SMC3 5.664416e-116  0.3705233 0.034 0.323 1.882512e-111     other
1139                    MED6 5.786055e-116 -1.3243959 0.008 0.273 1.922937e-111     other
1140                  PICALM 6.015590e-116  0.3466115 0.036 0.326 1.999221e-111     other
1141                    NAAA 7.074271e-116 -0.5554536 0.015 0.285 2.351063e-111     other
1142                   CEBPZ 8.241331e-116 -1.5733609 0.007 0.270 2.738924e-111     other
1143                 PITPNC1 1.129505e-115 -1.5090823 0.007 0.270 3.753796e-111     other
1144                 HNRNPAB 2.012354e-115 -0.2560207 0.018 0.292 6.687858e-111     other
1145                   GLRX5 2.129384e-115  0.2532819 0.040 0.333 7.076795e-111     other
1146                     LPP 2.224620e-115 -0.3027951 0.019 0.292 7.393301e-111     other
1147                   ABCF1 2.763988e-115 -0.5629914 0.015 0.285 9.185838e-111     other
1148                     MPG 2.827640e-115 -0.3144071 0.018 0.291 9.397379e-111     other
1149                 TMEM87A 3.030860e-115 -0.3806871 0.017 0.288 1.007276e-110     other
1150                   EIF3J 3.639690e-115 -0.3704045 0.018 0.290 1.209615e-110     other
1151                  GTF3C6 3.924498e-115  1.0761895 0.071 0.393 1.304268e-110     other
1152                  IL27RA 4.216147e-115 -1.7932301 0.005 0.265 1.401194e-110     other
1153                  ZNHIT3 4.286954e-115 -0.8182845 0.010 0.275 1.424726e-110     other
1154                   KCMF1 4.504896e-115  0.5113155 0.041 0.334 1.497157e-110     other
1155                  PSMD12 5.449381e-115 -0.4307560 0.016 0.287 1.811047e-110     other
1156                 SMARCC1 6.223481e-115 -0.3067300 0.017 0.288 2.068312e-110     other
1157              NUTM2B-AS1 7.565184e-115 -0.6195612 0.016 0.286 2.514213e-110     other
1158                    SDHC 7.777304e-115  0.3555278 0.028 0.309 2.584709e-110     other
1159                  UQCRC1 8.537332e-115  0.7756740 0.046 0.344 2.837297e-110     other
1160                    UBA2 8.581837e-115 -0.5769551 0.013 0.280 2.852088e-110     other
1161                  PPP3CA 8.865271e-115 -0.5929442 0.015 0.283 2.946284e-110     other
1162                   COPZ1 9.991198e-115  0.3055754 0.032 0.316 3.320475e-110     other
1163                  ARID5B 1.042127e-114 -1.8335668 0.009 0.271 3.463405e-110     other
1164                    CMC1 1.157151e-114 -1.8320185 0.015 0.283 3.845676e-110     other
1165                 BLOC1S6 1.416657e-114  0.4519260 0.042 0.337 4.708119e-110     other
1166                   HSBP1 1.426802e-114  0.7393749 0.069 0.388 4.741832e-110     other
1167           RP5-1171I10.5 1.573483e-114 -0.8084871 0.015 0.282 5.229313e-110     other
1168                   DDX27 1.657445e-114 -0.4918449 0.015 0.282 5.508352e-110     other
1169                    OPTN 1.991966e-114 -0.4184632 0.025 0.302 6.620101e-110     other
1170                   DEGS1 2.134976e-114 -0.6906709 0.012 0.277 7.095380e-110     other
1171                    TOB1 2.813763e-114 -1.1239339 0.011 0.275 9.351259e-110     other
1172                  TIMM8B 2.933342e-114 -0.2578955 0.018 0.289 9.748670e-110     other
1173                  NFATC3 3.052132e-114 -0.9118381 0.012 0.277 1.014346e-109     other
1174                   SRPRA 3.584111e-114 -0.3524549 0.018 0.289 1.191143e-109     other
1175                   PMPCB 3.772260e-114 -0.8357083 0.011 0.274 1.253673e-109     other
1176                   KLRD1 3.983033e-114 -2.1388725 0.015 0.283 1.323721e-109     other
1177                   NCOA3 4.232337e-114 -0.2606863 0.021 0.294 1.406575e-109     other
1178                   ITGB7 6.303485e-114 -1.1767690 0.013 0.278 2.094900e-109     other
1179                    FGL2 1.059187e-113 -0.2522442 0.064 0.376 3.520101e-109     other
1180                  NDUFA9 1.429317e-113 -0.3853503 0.013 0.277 4.750191e-109     other
1181                   TAF15 1.671349e-113 -0.4234239 0.015 0.282 5.554563e-109     other
1182                   PFDN4 1.945427e-113 -0.7939701 0.010 0.272 6.465433e-109     other
1183                    IRF3 1.947555e-113 -0.3135746 0.018 0.287 6.472506e-109     other
1184                  FCGR3A 1.957843e-113 -1.5166152 0.024 0.299 6.506695e-109     other
1185                  ZC3H13 1.987613e-113 -1.9344866 0.006 0.264 6.605634e-109     other
1186                  R3HDM2 2.031856e-113 -1.0730261 0.010 0.271 6.752669e-109     other
1187                 PRKAR2A 2.329345e-113 -0.9623419 0.009 0.269 7.741345e-109     other
1188              CSGALNACT2 3.875897e-113  0.2571615 0.032 0.313 1.288116e-108     other
1189                  ZFC3H1 3.919490e-113 -0.3450617 0.017 0.285 1.302603e-108     other
1190                   INTS6 4.109840e-113 -1.1383956 0.013 0.276 1.365864e-108     other
1191                   RBM22 4.910075e-113 -1.1641381 0.008 0.267 1.631814e-108     other
1192                   HERC1 7.229919e-113 -0.5044909 0.016 0.283 2.402791e-108     other
1193                  POLR2I 7.435957e-113 -0.2743123 0.016 0.283 2.471266e-108     other
1194                    RELA 8.587312e-113 -0.6033413 0.013 0.276 2.853907e-108     other
1195                  NDUFV1 8.668641e-113  0.2508537 0.026 0.301 2.880936e-108     other
1196                   NUCB1 8.922785e-113  1.0369775 0.082 0.410 2.965398e-108     other
1197                    MT2A 1.023183e-112  0.6803998 0.130 0.501 3.400447e-108     other
1198                   CNPY2 1.664536e-112 -0.3941974 0.015 0.280 5.531919e-108     other
1199                    AATF 1.813109e-112 -0.4552712 0.015 0.279 6.025687e-108     other
1200                   ASXL1 1.922543e-112 -0.2652818 0.020 0.290 6.389381e-108     other
1201                   GLUD1 2.009945e-112 -0.5289421 0.013 0.275 6.679851e-108     other
1202                   P2RY8 2.067379e-112 -1.7610647 0.007 0.264 6.870727e-108     other
1203                 RABGGTB 2.077869e-112 -1.3834713 0.008 0.266 6.905590e-108     other
1204                  PMAIP1 2.384210e-112 -1.1196221 0.018 0.284 7.923685e-108     other
1205                   DECR1 4.433527e-112  0.3434713 0.028 0.304 1.473438e-107     other
1206                    BRI3 4.449270e-112  0.3393180 0.178 0.601 1.478671e-107     other
1207                    DCXR 5.121001e-112 -0.2919461 0.014 0.277 1.701913e-107     other
1208                    SHFL 6.378181e-112  0.4069945 0.032 0.312 2.119725e-107     other
1209                   FOSL2 6.386757e-112  0.3874247 0.036 0.320 2.122575e-107     other
1210                  SCAMP2 6.790085e-112  0.5770915 0.037 0.322 2.256617e-107     other
1211                   CMTM7 6.953990e-112 -0.4475053 0.013 0.275 2.311089e-107     other
1212                   KARS1 8.638380e-112 -0.3680044 0.015 0.277 2.870879e-107     other
1213                   RNF13 8.644765e-112  0.3365602 0.029 0.306 2.873001e-107     other
1214                   CCAR1 9.714683e-112 -0.8279097 0.011 0.270 3.228578e-107     other
1215                  FGFBP2 9.798654e-112 -2.3317995 0.025 0.295 3.256485e-107     other
1216                   SZRD1 1.055085e-111  0.2905935 0.030 0.308 3.506471e-107     other
1217                   SETD2 1.287633e-111 -0.7568551 0.013 0.274 4.279320e-107     other
1218                  ARRDC2 1.357916e-111 -1.5148630 0.007 0.262 4.512899e-107     other
1219                   APPL1 1.839298e-111 -0.3383163 0.016 0.281 6.112721e-107     other
1220                  PBXIP1 1.885461e-111 -2.3897908 0.005 0.258 6.266142e-107     other
1221                    DAP3 2.494601e-111 -0.3023966 0.016 0.279 8.290558e-107     other
1222                  ZNF428 2.739037e-111 -0.3779370 0.018 0.284 9.102917e-107     other
1223                  SH3BP2 2.779999e-111  0.6955913 0.053 0.351 9.239049e-107     other
1224                   SYTL3 3.695315e-111 -1.9472423 0.009 0.265 1.228101e-106     other
1225                  CAVIN2 4.053207e-111  2.7792242 0.331 0.157 1.347043e-106     other
1226                  RNF145 4.436571e-111  0.2582288 0.028 0.302 1.474450e-106     other
1227                    TRAC 4.643657e-111 -1.2179367 0.022 0.291 1.543273e-106     other
1228                   SRSF6 6.370186e-111  0.7342111 0.051 0.347 2.117068e-106     other
1229                   PRKCH 6.436876e-111 -2.2653258 0.007 0.261 2.139231e-106     other
1230                   CCND3 6.467910e-111  1.1801824 0.181 0.606 2.149545e-106     other
1231                    HDGF 7.721058e-111  0.8111988 0.065 0.373 2.566016e-106     other
1232                  STARD7 7.910698e-111 -0.3376939 0.014 0.275 2.629041e-106     other
1233                    VSIR 8.607478e-111  0.7175907 0.149 0.541 2.860609e-106     other
1234                   PPDPF 8.837368e-111  1.1155860 0.252 0.763 2.937011e-106     other
1235                    CBX6 9.180647e-111 -0.8382027 0.013 0.272 3.051096e-106     other
1236                  GIMAP1 9.221234e-111 -0.7659938 0.013 0.273 3.064585e-106     other
1237                  UBE2Q1 1.102370e-110  0.7114298 0.046 0.336 3.663617e-106     other
1238                  PSMD11 1.197837e-110  0.2779740 0.028 0.302 3.980893e-106     other
1239                   SHOC2 1.217394e-110  0.3485248 0.032 0.310 4.045888e-106     other
1240                    CD3G 1.257644e-110 -1.7925147 0.015 0.276 4.179654e-106     other
1241                   NLRP1 1.258206e-110 -0.5089754 0.015 0.277 4.181522e-106     other
1242                 TMEM50A 1.451648e-110  1.3129447 0.190 0.627 4.824406e-106     other
1243                  MRPL10 1.462485e-110 -1.3198838 0.009 0.264 4.860423e-106     other
1244                  RASSF1 2.001495e-110 -0.4101809 0.020 0.286 6.651767e-106     other
1245                  ATXN10 2.187821e-110 -1.2597237 0.007 0.260 7.271006e-106     other
1246                  DNAJC1 2.484612e-110 -0.3603225 0.018 0.281 8.257360e-106     other
1247                  ARL2BP 2.578888e-110 -0.7669557 0.012 0.270 8.570675e-106     other
1248                    CCL4 2.634448e-110 -0.8415911 0.045 0.330 8.755325e-106     other
1249                 CCDC124 3.131497e-110 -0.2894015 0.014 0.274 1.040722e-105     other
1250                 ABHD14B 3.673137e-110 -0.6316054 0.015 0.274 1.220730e-105     other
1251                   RBBP6 3.813025e-110  1.1997840 0.112 0.464 1.267221e-105     other
1252                    ABI1 4.452244e-110  0.4577819 0.035 0.314 1.479659e-105     other
1253                   RAMAC 5.285685e-110 -0.3341824 0.016 0.278 1.756645e-105     other
1254                    PRKX 5.719626e-110 -0.6338658 0.014 0.273 1.900860e-105     other
1255                  STING1 6.233934e-110 -0.8895184 0.011 0.268 2.071786e-105     other
1256                  MAP2K3 6.475627e-110  0.8761957 0.086 0.413 2.152110e-105     other
1257                   MED30 9.335111e-110 -1.2565263 0.008 0.261 3.102431e-105     other
1258                  MTMR14 9.722489e-110  0.2548982 0.025 0.295 3.231172e-105     other
1259                  DGCR6L 1.142760e-109 -0.6473871 0.011 0.268 3.797849e-105     other
1260                  MAN1A2 1.214378e-109 -0.7974674 0.012 0.268 4.035865e-105     other
1261                  POLR2A 1.346643e-109 -0.4011690 0.016 0.277 4.475432e-105     other
1262                    TMC8 1.431957e-109 -0.3431691 0.018 0.279 4.758966e-105     other
1263                    CUL5 1.486705e-109 -1.0656257 0.010 0.264 4.940916e-105     other
1264                   RRP7A 1.613286e-109 -0.3681483 0.015 0.275 5.361595e-105     other
1265                    SKIL 2.347984e-109 -0.8471906 0.011 0.267 7.803290e-105     other
1266                  CD2BP2 4.123875e-109 -0.4615618 0.015 0.272 1.370529e-104     other
1267                    PAN3 4.216541e-109 -0.5325641 0.013 0.270 1.401325e-104     other
1268                  ZBTB20 4.338621e-109 -0.6748576 0.014 0.271 1.441897e-104     other
1269                  AKAP8L 4.747798e-109 -0.6168965 0.011 0.266 1.577883e-104     other
1270                   SUMF2 5.863077e-109 -0.3917183 0.016 0.275 1.948535e-104     other
1271                  POLR2G 6.668433e-109  0.9107087 0.060 0.361 2.216187e-104     other
1272                   PSMD2 7.768375e-109  0.6096117 0.042 0.326 2.581742e-104     other
1273                  POLR2E 8.180188e-109  0.9393990 0.065 0.370 2.718604e-104     other
1274                   ACIN1 9.095142e-109 -0.5987651 0.014 0.271 3.022679e-104     other
1275                 ATP6AP1 1.048140e-108  0.6592630 0.043 0.327 3.483388e-104     other
1276                  ZNF791 1.057306e-108 -1.1819322 0.009 0.261 3.513851e-104     other
1277                     DUT 1.282093e-108  0.4318151 0.033 0.308 4.260909e-104     other
1278                    CTCF 1.472393e-108 -0.3112565 0.019 0.280 4.893352e-104     other
1279                   SENP6 1.788984e-108 -0.3789537 0.018 0.277 5.945509e-104     other
1280                    XAF1 1.946548e-108  0.3823810 0.042 0.326 6.469159e-104     other
1281                   ACBD3 1.993867e-108 -0.3273585 0.016 0.275 6.626417e-104     other
1282                CDC42EP3 2.113020e-108  0.5550478 0.050 0.340 7.022412e-104     other
1283                 FAM200B 2.467003e-108 -0.3694434 0.016 0.275 8.198837e-104     other
1284                    NUMB 2.595638e-108  0.2846039 0.030 0.302 8.626344e-104     other
1285                  RAB11B 4.078738e-108  0.5440956 0.036 0.312 1.355528e-103     other
1286                  CGGBP1 4.185387e-108  0.5481291 0.041 0.322 1.390971e-103     other
1287                  LGALS3 5.131762e-108  0.2866889 0.101 0.440 1.705490e-103     other
1288                   ARIH2 5.158652e-108 -0.7207432 0.011 0.265 1.714426e-103     other
1289                   STAT4 5.197561e-108 -1.6466099 0.008 0.257 1.727357e-103     other
1290                    TSR3 5.505155e-108 -0.6376982 0.011 0.265 1.829583e-103     other
1291            RP11-160E2.6 5.857505e-108 -1.6872977 0.008 0.258 1.946683e-103     other
1292                    PDXK 6.180367e-108  0.2944066 0.027 0.295 2.053983e-103     other
1293                 TMEM219 8.282588e-108  1.2767287 0.140 0.515 2.752635e-103     other
1294                  ARPP19 8.898530e-108  0.2754337 0.031 0.302 2.957338e-103     other
1295                   STK24 2.460029e-107  0.8364630 0.051 0.339 8.175659e-103     other
1296                   GON4L 2.610801e-107 -0.4599601 0.016 0.272 8.676736e-103     other
1297                    IST1 3.045604e-107  0.3852178 0.030 0.300 1.012176e-102     other
1298                    GBP4 3.304138e-107 -0.7514682 0.014 0.268 1.098097e-102     other
1299                    SMU1 3.321938e-107 -1.0304638 0.009 0.258 1.104013e-102     other
1300                  RNF130 3.326059e-107  0.3338753 0.067 0.370 1.105382e-102     other
1301                  OCIAD2 4.847835e-107 -1.6479088 0.007 0.254 1.611129e-102     other
1302                   AP2S1 6.361129e-107  0.9948262 0.160 0.555 2.114058e-102     other
1303                   CNTRL 7.479408e-107 -1.4035942 0.008 0.255 2.485706e-102     other
1304                  SFT2D1 8.504998e-107  0.5104780 0.035 0.308 2.826551e-102     other
1305                    PHF1 1.264789e-106 -1.3442729 0.010 0.258 4.203398e-102     other
1306                   COX20 1.474799e-106 -0.8528144 0.011 0.262 4.901348e-102     other
1307                  MRPL28 1.527944e-106  0.3339590 0.025 0.289 5.077969e-102     other
1308                   TXNL1 1.782953e-106  0.8889085 0.051 0.338 5.925467e-102     other
1309                    PARL 1.979723e-106 -0.9514988 0.009 0.257 6.579413e-102     other
1310                  MAP2K7 2.171245e-106 -0.5447965 0.015 0.268 7.215916e-102     other
1311                     ZYX 2.894820e-106  0.7862562 0.088 0.409 9.620644e-102     other
1312                  CACYBP 3.163451e-106  0.6930959 0.035 0.306 1.051341e-101     other
1313                   SYPL1 3.402636e-106 -0.2942947 0.015 0.267 1.130832e-101     other
1314                TRAPPC6A 3.630594e-106 -1.1971778 0.009 0.256 1.206592e-101     other
1315                     PHB 4.538254e-106  0.6087830 0.028 0.293 1.508243e-101     other
1316                   NCBP2 4.613474e-106 -0.5626479 0.013 0.265 1.533242e-101     other
1317                   ITGAE 4.779966e-106 -0.5231815 0.013 0.264 1.588574e-101     other
1318                   ARAP2 4.822343e-106 -0.9806179 0.011 0.260 1.602658e-101     other
1319                   SF3B4 5.972153e-106 -0.4639181 0.013 0.265 1.984785e-101     other
1320                   LYRM2 7.107617e-106 -0.3871707 0.015 0.267 2.362145e-101     other
1321                   SCNM1 8.665824e-106 -0.3877789 0.015 0.266 2.880000e-101     other
1322                   KDM6B 9.046797e-106  0.5585585 0.044 0.324 3.006612e-101     other
1323                 CCDC88B 1.314271e-105  0.4450405 0.029 0.294 4.367849e-101     other
1324                  TTC39C 1.346366e-105 -1.0350964 0.010 0.256 4.474511e-101     other
1325                    TCP1 1.554896e-105  0.3238805 0.029 0.294 5.167542e-101     other
1326                    PNKD 1.581585e-105  0.4559661 0.032 0.300 5.256240e-101     other
1327                   TCOF1 1.814408e-105 -1.2624622 0.010 0.256 6.030003e-101     other
1328                    RAC1 1.832370e-105  0.7491288 0.237 0.722 6.089698e-101     other
1329                   CMPK1 2.062516e-105  1.1439996 0.107 0.444 6.854565e-101     other
1330                C1orf122 2.537316e-105 -0.2981130 0.016 0.268 8.432517e-101     other
1331                   RIOK3 3.195488e-105  1.1182179 0.122 0.474 1.061988e-100     other
1332                   CPNE3 3.289077e-105 -0.4226429 0.013 0.262 1.093092e-100     other
1333                   VTI1B 4.660268e-105  0.6470355 0.044 0.322 1.548794e-100     other
1334                    GZMB 4.778136e-105 -1.4955105 0.041 0.313 1.587966e-100     other
1335                    UBN1 5.186675e-105  0.4629849 0.033 0.301 1.723740e-100     other
1336                     JPX 5.292771e-105 -1.1101693 0.010 0.257 1.759000e-100     other
1337                  CRYBG1 6.115680e-105 -0.8456982 0.012 0.260 2.032485e-100     other
1338                   RPAIN 6.400655e-105 -0.6392807 0.012 0.260 2.127194e-100     other
1339                   ATXN1 7.159063e-105 -0.3883314 0.015 0.265 2.379243e-100     other
1340                ANKRD13A 7.484416e-105 -0.3255340 0.015 0.266 2.487371e-100     other
1341                   STAT6 8.115090e-105  0.4498016 0.030 0.295 2.696969e-100     other
1342                  MRPL21 1.032653e-104 -0.2930639 0.014 0.263 3.431918e-100     other
1343                  MBTPS1 1.202657e-104 -0.3624474 0.015 0.266 3.996909e-100     other
1344                    RHEB 1.474888e-104  0.8983691 0.058 0.349 4.901642e-100     other
1345                    SQOR 1.678460e-104  0.5701021 0.037 0.307 5.578195e-100     other
1346                   DEDD2 2.542551e-104 -0.5265423 0.012 0.259 8.449915e-100     other
1347                   APLP2 3.148564e-104  0.4690660 0.133 0.496  1.046394e-99     other
1348                   SF3A2 3.763861e-104 -0.6107618 0.015 0.264  1.250881e-99     other
1349                  FKBP1A 3.851206e-104  0.9696736 0.177 0.585  1.279910e-99     other
1350                 FAM118A 5.026680e-104 -0.6012123 0.012 0.259  1.670567e-99     other
1351                  EIF4G1 6.299977e-104 -0.2913544 0.016 0.267  2.093734e-99     other
1352                    CHD9 7.406124e-104  0.5545795 0.036 0.304  2.461351e-99     other
1353                    PEPD 8.837918e-104  0.3129123 0.023 0.280  2.937194e-99     other
1354                    DENR 9.666792e-104 -0.5247454 0.013 0.259  3.212662e-99     other
1355                    IL4R 1.050641e-103 -0.7974272 0.012 0.258  3.491700e-99     other
1356                   UBE2K 1.078533e-103  0.3446488 0.031 0.295  3.584396e-99     other
1357                MARCKSL1 1.099251e-103 -0.7505397 0.014 0.262  3.653250e-99     other
1358                  LGALS9 1.222368e-103  0.5016413 0.041 0.313  4.062417e-99     other
1359                   AP3B1 1.340288e-103 -0.4235803 0.013 0.260  4.454313e-99     other
1360                SECISBP2 1.388818e-103  0.5159940 0.039 0.311  4.615597e-99     other
1361                DYNC1LI1 1.430892e-103 -0.3740641 0.016 0.265  4.755426e-99     other
1362                   KPNA6 1.566669e-103 -0.7521067 0.011 0.255  5.206667e-99     other
1363                   MED13 1.624160e-103 -0.5121564 0.014 0.261  5.397732e-99     other
1364                 CCDC107 1.786937e-103 -0.2759703 0.017 0.267  5.938708e-99     other
1365                    NASP 1.995118e-103  0.3395728 0.027 0.285  6.630576e-99     other
1366                  SAMSN1 2.267307e-103  1.2765555 0.088 0.405  7.535168e-99     other
1367                 IFI27L2 2.696124e-103  1.1812871 0.089 0.407  8.960298e-99     other
1368                  NAP1L4 2.792094e-103  0.6991494 0.055 0.339  9.279246e-99     other
1369                    SRA1 3.316223e-103  0.6166500 0.032 0.296  1.102113e-98     other
1370                  VPS26A 5.441453e-103 -0.3697053 0.018 0.267  1.808412e-98     other
1371                   ECHS1 5.891613e-103 -0.2534368 0.014 0.260  1.958019e-98     other
1372                   HOOK3 7.327122e-103  0.3449361 0.032 0.295  2.435096e-98     other
1373                    TET2 7.971390e-103 -0.4183320 0.017 0.266  2.649212e-98     other
1374                  CHMP2B 9.414491e-103  0.5147716 0.029 0.289  3.128812e-98     other
1375                 C9orf16 1.039924e-102  1.3741659 0.193 0.616  3.456083e-98     other
1376                  TEX264 1.096576e-102 -0.2770250 0.015 0.261  3.644360e-98     other
1377                  SDF2L1 1.433593e-102  0.6353897 0.040 0.309  4.764403e-98     other
1378                  ERGIC1 1.739790e-102  0.7176228 0.037 0.304  5.782019e-98     other
1379                   PTK2B 1.930245e-102  0.8675524 0.042 0.315  6.414976e-98     other
1380                    RARA 1.934818e-102 -0.2818586 0.016 0.264  6.430176e-98     other
1381                 N4BP2L1 2.013244e-102  0.2510022 0.030 0.291  6.690815e-98     other
1382                    CHD8 2.073724e-102 -0.4821956 0.012 0.256  6.891814e-98     other
1383                     GAK 2.202429e-102  0.3771506 0.025 0.280  7.319552e-98     other
1384                    DCP2 2.270573e-102  0.5863874 0.036 0.301  7.546023e-98     other
1385                 CDK2AP2 2.589305e-102 -0.2991105 0.020 0.271  8.605295e-98     other
1386                  CLDND1 2.700964e-102 -0.4548550 0.017 0.265  8.976384e-98     other
1387                  ACADVL 3.534834e-102  0.8088797 0.046 0.320  1.174767e-97     other
1388                   SUZ12 4.187637e-102 -0.3478365 0.016 0.262  1.391719e-97     other
1389                   STX11 4.338579e-102  0.3603354 0.044 0.316  1.441883e-97     other
1390                  PTGER2 5.014402e-102 -0.2578461 0.018 0.266  1.666486e-97     other
1391                  TGFBR2 6.151734e-102 -0.5485658 0.015 0.259  2.044467e-97     other
1392                  DNAJC2 6.195775e-102 -0.3102281 0.014 0.258  2.059104e-97     other
1393                  CDC123 6.766983e-102 -0.8359200 0.011 0.252  2.248939e-97     other
1394                 ARFGAP3 9.875282e-102 -0.3549222 0.015 0.260  3.281951e-97     other
1395                  CHST12 1.058342e-101 -0.7578166 0.019 0.268  3.517294e-97     other
1396                  PHYKPL 1.443894e-101 -0.5874585 0.011 0.253  4.798638e-97     other
1397                  GALNT1 1.646737e-101 -0.7389027 0.011 0.252  5.472766e-97     other
1398                  ZBTB43 1.959468e-101  0.6531022 0.039 0.305  6.512095e-97     other
1399                   NINJ1 2.412679e-101  0.8365000 0.082 0.390  8.018296e-97     other
1400                    SND1 2.810701e-101 -0.4021073 0.013 0.255  9.341085e-97     other
1401                    ITPA 3.767160e-101 -0.5783807 0.011 0.252  1.251978e-96     other
1402                   TENT2 5.522375e-101 -0.8365565 0.011 0.252  1.835306e-96     other
1403                   PSME4 6.120596e-101 -0.3161847 0.020 0.267  2.034119e-96     other
1404                  ZNF652 6.315872e-101 -0.5010044 0.013 0.255  2.099017e-96     other
1405                    CUX1 7.519532e-101  0.5911510 0.039 0.305  2.499041e-96     other
1406                  ZNF276 7.786799e-101 -0.6668887 0.012 0.253  2.587865e-96     other
1407                    GNLY 7.885054e-101 -1.8832021 0.084 0.356  2.620519e-96     other
1408                   SBNO1 8.705298e-101 -0.3966815 0.015 0.259  2.893119e-96     other
1409                  POLR2C 8.969102e-101 -0.3052054 0.014 0.256  2.980791e-96     other
1410                     ZFX 1.023684e-100 -0.6258332 0.011 0.251  3.402112e-96     other
1411                   PLIN2 1.682279e-100  0.4336018 0.032 0.291  5.590886e-96     other
1412                  FAM50A 1.712977e-100  0.3935910 0.029 0.284  5.692909e-96     other
1413                    CSTA 4.513556e-100 -0.2647947 0.055 0.333  1.500035e-95     other
1414                    DEF6 4.725253e-100 -0.2737176 0.016 0.259  1.570391e-95     other
1415                   SPAG9 6.498464e-100  0.8076785 0.039 0.303  2.159699e-95     other
1416                    LY86 8.340602e-100  0.2634415 0.032 0.290  2.771916e-95     other
1417                   TADA3 8.482514e-100  0.4322378 0.035 0.295  2.819079e-95     other
1418                  NDUFA6  1.096639e-99  1.3782743 0.119 0.455  3.644571e-95     other
1419                   IFT20  1.170871e-99 -0.3618070 0.014 0.254  3.891274e-95     other
1420                  CHST11  1.461825e-99 -0.3409888 0.014 0.254  4.858229e-95     other
1421                     SMS  1.586424e-99  0.4168840 0.032 0.290  5.272321e-95     other
1422                  LMBRD1  2.391583e-99 -0.3396502 0.016 0.257  7.948187e-95     other
1423                  CAPZA2  3.530283e-99  1.2647019 0.134 0.485  1.173254e-94     other
1424                    GYG1  3.921775e-99  0.5798484 0.028 0.280  1.303363e-94     other
1425                KIAA0930  4.291827e-99 -0.4578712 0.017 0.259  1.426346e-94     other
1426                  PPP4R2  4.459543e-99  0.3663401 0.027 0.278  1.482085e-94     other
1427                    EHD1  5.309128e-99  0.6570761 0.047 0.316  1.764436e-94     other
1428                     SLK  6.541966e-99 -0.3822941 0.014 0.253  2.174157e-94     other
1429                    UBL3  7.922577e-99 -0.3064644 0.014 0.253  2.632989e-94     other
1430               KIDINS220  8.526217e-99 -0.3334823 0.016 0.256  2.833603e-94     other
1431                   VPS36  1.041526e-98 -0.5409720 0.013 0.250  3.461409e-94     other
1432                     SRM  1.049047e-98  0.7265141 0.029 0.282  3.486403e-94     other
1433                   UBE2S  1.106619e-98  0.4121484 0.029 0.281  3.677738e-94     other
1434                   NAA50  1.128036e-98  0.2769266 0.023 0.271  3.748915e-94     other
1435                   DNM1L  1.178744e-98 -0.4216454 0.015 0.253  3.917439e-94     other
1436                    RELB  1.326643e-98  0.3212586 0.025 0.274  4.408964e-94     other
1437                ARHGAP26  1.455172e-98 -0.2994855 0.017 0.258  4.836118e-94     other
1438                  SH3BP5  1.488883e-98  0.4096541 0.036 0.295  4.948155e-94     other
1439                    CPVL  1.549002e-98 -0.5538688 0.034 0.291  5.147953e-94     other
1440                 PIP4K2A  1.940352e-98  1.0144838 0.088 0.394  6.448565e-94     other
1441                  SEMA4D  2.667546e-98  0.3696011 0.031 0.285  8.865321e-94     other
1442                    DDB1  4.169413e-98 -0.3390283 0.015 0.252  1.385663e-93     other
1443                   STK38  4.651776e-98 -0.4660171 0.015 0.252  1.545971e-93     other
1444                 TNFSF10  4.787369e-98  0.2756514 0.034 0.290  1.591034e-93     other
1445                    FLNA  5.669215e-98  0.8640690 0.248 0.729  1.884107e-93     other
1446                 PPP4R3B  5.844641e-98 -0.2546569 0.017 0.257  1.942408e-93     other
1447                   RNF10  7.154182e-98  0.5481775 0.040 0.301  2.377621e-93     other
1448                   LSM12  1.675422e-97  0.3907723 0.026 0.273  5.568096e-93     other
1449                    RAF1  1.677865e-97  1.0228875 0.044 0.309  5.576216e-93     other
1450                    WAPL  1.834420e-97 -0.2868047 0.015 0.252  6.096513e-93     other
1451                  RASAL3  1.900588e-97 -0.5285733 0.015 0.251  6.316413e-93     other
1452           RP11-1143G9.4  5.421427e-97 -0.8927709 0.074 0.356  1.801757e-92     other
1453                    UBR4  9.258650e-97  0.4782184 0.032 0.283  3.077020e-92     other
1454                 PHF20L1  1.278280e-96  0.8162983 0.048 0.313  4.248235e-92     other
1455                    EMC3  1.430668e-96  1.2381675 0.106 0.425  4.754681e-92     other
1456                MIR23AHG  2.630484e-96 -0.4623827 0.019 0.258  8.742151e-92     other
1457               PSMB8-AS1  2.955689e-96 -0.2587978 0.018 0.255  9.822937e-92     other
1458                   STAU1  3.299556e-96  0.3988595 0.030 0.278  1.096575e-91     other
1459                   ZAP70  6.919001e-96 -0.4844368 0.020 0.258  2.299461e-91     other
1460                   CHMP3  6.983628e-96  0.4411679 0.030 0.279  2.320939e-91     other
1461                    CREM  1.346807e-95 -1.1496642 0.016 0.250  4.475978e-91     other
1462                  MRPL55  1.428584e-95  0.3363873 0.022 0.261  4.747757e-91     other
1463                  NECAP1  2.014662e-95  0.3840642 0.024 0.265  6.695527e-91     other
1464                    EMP3  2.158195e-95  0.4522608 0.292 0.831  7.172546e-91     other
1465                    MTPN  2.407849e-95  1.2884619 0.204 0.626  8.002246e-91     other
1466                   PRDX6  2.518601e-95  1.4096497 0.179 0.572  8.370318e-91     other
1467                   NLRC5  3.476678e-95  0.3521672 0.027 0.270  1.155439e-90     other
1468                    PFKL  3.616281e-95  0.3702971 0.026 0.269  1.201835e-90     other
1469                    CAPG  6.317292e-95  0.7027146 0.060 0.333  2.099489e-90     other
1470                   ADAM8  1.164097e-94  0.4602349 0.027 0.269  3.868760e-90     other
1471                   WARS1  1.440388e-94  0.3397626 0.052 0.317  4.786985e-90     other
1472                   BLVRA  1.468829e-94  0.6707637 0.036 0.286  4.881507e-90     other
1473                    SLBP  2.481609e-94  0.2882083 0.028 0.271  8.247379e-90     other
1474                SLC25A39  3.031220e-94 -0.6385362 0.018 0.252  1.007396e-89     other
1475                 FAM91A1  3.466082e-94  0.2542240 0.022 0.259  1.151918e-89     other
1476                   HSPD1  5.371192e-94  1.3180509 0.041 0.296  1.785062e-89     other
1477                  BABAM1  5.869516e-94  0.4025345 0.026 0.266  1.950675e-89     other
1478                  JARID2  6.621508e-94  0.9602399 0.058 0.329  2.200592e-89     other
1479                 C7orf50  6.699804e-94  0.6050336 0.041 0.294  2.226613e-89     other
1480                 CARHSP1  1.518851e-93  0.2792675 0.023 0.261  5.047750e-89     other
1481                  IL17RA  1.518964e-93  0.4724652 0.042 0.296  5.048124e-89     other
1482                   USP47  1.567744e-93  0.3064795 0.024 0.262  5.210239e-89     other
1483                   NAA38  2.095247e-93  1.4073638 0.122 0.450  6.963345e-89     other
1484                   FLOT1  2.878863e-93  0.6970489 0.035 0.282  9.567612e-89     other
1485                  RASSF2  1.433047e-92  0.3928240 0.031 0.274  4.762588e-88     other
1486                   COPS3  2.639436e-92  0.4892494 0.027 0.265  8.771902e-88     other
1487                   YWHAE  2.934520e-92  1.3692269 0.114 0.433  9.752585e-88     other
1488                     NMI  3.339031e-92  0.7944937 0.038 0.286  1.109694e-87     other
1489                RABGAP1L  3.375019e-92  0.6853085 0.053 0.315  1.121654e-87     other
1490                   HMGB1  6.225490e-92  0.6580470 0.325 0.907  2.068979e-87     other
1491                  S100A4  6.650917e-92 -0.3930830 0.434 0.841  2.210366e-87     other
1492                    NRDC  6.775073e-92  0.6944127 0.044 0.298  2.251628e-87     other
1493                    PAG1  7.043950e-92  0.9761581 0.042 0.294  2.340986e-87     other
1494                  WASHC1  7.112768e-92  0.3565432 0.025 0.261  2.363857e-87     other
1495                   AGFG1  8.085057e-92  0.8249495 0.041 0.292  2.686988e-87     other
1496                    PLK3  1.886380e-91  1.0375589 0.044 0.297  6.269195e-87     other
1497                   MRPS6  2.356950e-91  0.4142797 0.024 0.258  7.833089e-87     other
1498                    OAZ2  2.495312e-91  0.5158586 0.029 0.267  8.292921e-87     other
1499                   DCTN2  3.162211e-91  0.7973252 0.043 0.294  1.050929e-86     other
1500                     FTL  3.871919e-91  0.8746855 0.869 0.988  1.286794e-86     other
1501                 CLPTM1L  4.560257e-91  0.2959844 0.022 0.253  1.515556e-86     other
1502                   RNPEP  6.520560e-91  0.3619669 0.025 0.259  2.167043e-86     other
1503                  PPP6R1  6.721747e-91  0.6098553 0.041 0.288  2.233905e-86     other
1504                  GRPEL1  1.008681e-90  0.3044624 0.021 0.251  3.352249e-86     other
1505                   MED15  1.102247e-90  0.2798917 0.023 0.254  3.663208e-86     other
1506                   ARPC5  2.179666e-90  1.0046753 0.211 0.630  7.243901e-86     other
1507                   DUSP6  2.514565e-90 -0.4057005 0.049 0.303  8.356907e-86     other
1508                    ETV6  2.767689e-90  0.4760358 0.028 0.263  9.198137e-86     other
1509                 ZDHHC20  3.155319e-90  0.4062813 0.027 0.262  1.048639e-85     other
1510                  CDKN2D  4.120702e-90  1.1375848 0.211 0.624  1.369474e-85     other
1511                  CYB5R4  4.561744e-90  0.3743296 0.025 0.257  1.516050e-85     other
1512                    DGKD  5.343583e-90  0.3786551 0.036 0.277  1.775886e-85     other
1513                   ATG2A  7.871104e-90  0.6847821 0.040 0.285  2.615883e-85     other
1514                 SERINC3  7.889552e-90  0.8634530 0.042 0.290  2.622014e-85     other
1515                   IGF2R  7.928673e-90  0.3480233 0.027 0.261  2.635015e-85     other
1516                    RGS2  9.347255e-90  0.7690207 0.084 0.369  3.106467e-85     other
1517                   H2AC6  9.898441e-90  2.7406036 0.334 0.180  3.289648e-85     other
1518                    NCF2  1.492291e-89  0.4047138 0.062 0.327  4.959479e-85     other
1519                  DNAJC4  2.391691e-89  0.5762558 0.033 0.271  7.948545e-85     other
1520                   LACTB  5.471365e-89  0.4462449 0.028 0.261  1.818353e-84     other
1521                  S100A6  6.048476e-89 -0.3216474 0.415 0.866  2.010150e-84     other
1522                   IFI35  9.152172e-89  0.5694248 0.030 0.265  3.041633e-84     other
1523                     GPI  1.225207e-88  0.6177279 0.030 0.264  4.071853e-84     other
1524                   DDIT3  1.395714e-88  0.4928647 0.030 0.264  4.638517e-84     other
1525                  UBQLN1  1.416176e-88  0.4712626 0.023 0.251  4.706518e-84     other
1526                   PLIN3  2.582223e-88  0.5821038 0.033 0.269  8.581760e-84     other
1527                   TMOD3  2.771191e-88  0.9900310 0.053 0.308  9.209775e-84     other
1528                  DENND3  2.801794e-88  1.0284938 0.045 0.292  9.311482e-84     other
1529                    LSM1  3.162890e-88  0.9155791 0.052 0.305  1.051155e-83     other
1530                   SKAP2  3.210842e-88  0.8528526 0.062 0.325  1.067091e-83     other
1531                   GNA13  3.604209e-88  0.5847395 0.029 0.261  1.197823e-83     other
1532                   UBE2F  4.128220e-88  0.9141372 0.060 0.320  1.371973e-83     other
1533                    CTSB  6.182907e-88  0.7837045 0.108 0.412  2.054827e-83     other
1534                  RILPL2  8.151078e-88  1.3213812 0.108 0.412  2.708929e-83     other
1535                ATP6V1E1  9.465137e-88  0.9786675 0.049 0.298  3.145643e-83     other
1536                   SIAH2  1.693637e-87  0.6584546 0.047 0.294  5.628632e-83     other
1537                    GAS7  2.028964e-87  0.5114722 0.036 0.273  6.743058e-83     other
1538                    G6PD  2.934016e-87  0.6667654 0.039 0.278  9.750910e-83     other
1539                   JMJD6  3.864037e-87  0.4396293 0.029 0.260  1.284174e-82     other
1540                    LST1  8.041516e-87 -0.4225939 0.124 0.428  2.672517e-82     other
1541                   PCNX1  1.690363e-86  0.6906638 0.025 0.250  5.617751e-82     other
1542                   MLXIP  1.775415e-86  0.5157182 0.027 0.255  5.900415e-82     other
1543                  DUSP22  4.082144e-86  0.3777069 0.028 0.255  1.356660e-81     other
1544                 SLC66A2  1.350697e-85  0.9575686 0.042 0.282  4.488905e-81     other
1545                     CFP  1.959271e-85  0.3806792 0.059 0.313  6.511441e-81     other
1546                ATP6V1B2  1.979911e-85  0.6686598 0.033 0.263  6.580037e-81     other
1547                   TIMP2  2.657841e-85  0.6428794 0.049 0.293  8.833069e-81     other
1548               MTRNR2L12  5.532724e-85  1.1831379 0.248 0.697  1.838746e-80     other
1549                   HACD4  8.958361e-85  1.0416828 0.074 0.339  2.977222e-80     other
1550                   UPF3A  6.062837e-84  0.7008754 0.039 0.272  2.014923e-79     other
1551                  LILRB3  7.345448e-84  0.3789159 0.037 0.268  2.441186e-79     other
1552                    WDR1  8.520196e-84  1.3541951 0.155 0.495  2.831602e-79     other
1553                    ARF4  2.820184e-83  0.9793534 0.048 0.288  9.372600e-79     other
1554                    CMC2  4.008161e-83  0.6963096 0.039 0.270  1.332072e-78     other
1555                   PCMT1  2.644468e-82  1.3288176 0.075 0.336  8.788626e-78     other
1556                  DYNLL2  5.359621e-82  0.5569622 0.032 0.254  1.781217e-77     other
1557                C19orf38  9.144059e-82  0.5367664 0.033 0.256  3.038936e-77     other
1558                   TOMM5  1.341413e-81  0.7800166 0.034 0.258  4.458053e-77     other
1559                    LMO2  1.406972e-81  0.4112779 0.036 0.262  4.675932e-77     other
1560                    RHOC  1.485438e-81  0.5338890 0.068 0.321  4.936706e-77     other
1561                  PLSCR1  2.473113e-81  1.1214835 0.057 0.301  8.219144e-77     other
1562                   ARAP1  2.851110e-81  0.6463925 0.030 0.250  9.475378e-77     other
1563                  CPPED1  4.158166e-81  0.7625632 0.042 0.273  1.381925e-76     other
1564                  PECAM1  4.987643e-81  0.7428598 0.065 0.316  1.657593e-76     other
1565                   RAB1B  1.942958e-80  1.1020025 0.051 0.288  6.457227e-76     other
1566                TNFSF13B  2.341071e-80  0.5301372 0.067 0.317  7.780315e-76     other
1567                   BLVRB  2.843981e-80  0.9349003 0.082 0.346  9.451686e-76     other
1568                  MT-ND6  1.473407e-79  0.5415942 0.032 0.250  4.896720e-75        mt
1569                   ORAI2  2.757803e-79  0.6232389 0.044 0.273  9.165281e-75     other
1570                   IGSF6  5.667603e-79  0.5170791 0.042 0.268  1.883571e-74     other
1571                    RBX1  6.243725e-79  1.6763037 0.188 0.553  2.075040e-74     other
1572                   CNIH4  9.776793e-79  1.0119919 0.037 0.257  3.249219e-74     other
1573                  MARCKS  1.808200e-78  0.4326316 0.085 0.346  6.009373e-74     other
1574                  CLEC7A  2.364259e-78  0.5216337 0.055 0.291  7.857377e-74     other
1575                   PTPRJ  2.861761e-78  0.9226780 0.044 0.270  9.510775e-74     other
1576                  LILRB2  7.452635e-78  0.5320869 0.052 0.284  2.476809e-73     other
1577                    CMIP  9.922059e-78  1.2631495 0.084 0.345  3.297497e-73     other
1578                 AKIRIN2  2.385208e-77  1.3950529 0.107 0.385  7.927000e-73     other
1579                    IRF7  2.811779e-77  0.8072088 0.037 0.254  9.344665e-73     other
1580                C12orf75  3.654640e-77  0.6083836 0.093 0.359  1.214583e-72     other
1581                   RAB31  8.452398e-77  0.8136630 0.069 0.314  2.809070e-72     other
1582                   NORAD  1.525636e-76  1.4833465 0.090 0.353  5.070300e-72     other
1583                   ITGAX  4.018186e-76  0.9367194 0.044 0.266  1.335404e-71     other
1584                   CYTOR  6.443717e-76  1.1083871 0.081 0.335  2.141505e-71     other
1585                  HPCAL1  1.239900e-75  0.8665292 0.039 0.255  4.120683e-71     other
1586                MTRNR2L8  2.090112e-75  1.3235843 0.096 0.361  6.946278e-71     other
1587                    GNAQ  3.320900e-75  0.8301267 0.042 0.261  1.103668e-70     other
1588                    SPI1  5.018920e-75  0.3290768 0.114 0.394  1.667988e-70     other
1589                   YIPF3  9.206373e-75  1.0601071 0.045 0.264  3.059646e-70     other
1590                   PRDX3  4.656214e-74  1.0593692 0.039 0.252  1.547446e-69     other
1591                  NDUFS4  4.765831e-74  1.1364272 0.041 0.256  1.583876e-69     other
1592                     PGD  6.747238e-74  0.9009163 0.070 0.309  2.242377e-69     other
1593                  LILRA5  1.351287e-73  0.5230352 0.052 0.275  4.490867e-69     other
1594                  TUBB4B  1.711876e-73  1.3009317 0.108 0.381  5.689247e-69     other
1595                 S100A11  3.244244e-73  0.2706339 0.245 0.627  1.078192e-68     other
1596                    CTSD  1.427253e-72  0.8734557 0.240 0.646  4.743333e-68     other
1597                   FKBP5  2.514071e-72  1.2180422 0.066 0.299  8.355264e-68     other
1598                  TBXAS1  3.994048e-72  0.8888474 0.044 0.258  1.327382e-67     other
1599                   NUTF2  4.238875e-72  1.2205350 0.063 0.292  1.408748e-67     other
1600                 SEC14L1  4.643069e-72  1.2851148 0.079 0.322  1.543078e-67     other
1601                    PLEK  1.351589e-71  1.0681590 0.194 0.548  4.491871e-67     other
1602                     GRN  1.683023e-71  0.4539327 0.126 0.410  5.593359e-67     other
1603                   ASAP1  1.878919e-71  0.8898111 0.046 0.258  6.244399e-67     other
1604                    PPBP  2.462363e-71  2.3786067 0.394 0.266  8.183417e-67     other
1605                   ISG15  4.453712e-71  1.5322162 0.134 0.424  1.480147e-66     other
1606                   ISCA1  4.926472e-71  1.3088342 0.083 0.327  1.637264e-66     other
1607                   IFRD1  6.763218e-71  1.0830681 0.055 0.274  2.247688e-66     other
1608                  PTPN12  2.081243e-70  1.0865150 0.072 0.306  6.916802e-66     other
1609                SERPINB1  3.614162e-70  1.1771298 0.219 0.599  1.201131e-65     other
1610                 RASGRP2  9.296915e-70  1.7935661 0.146 0.446  3.089737e-65     other
1611                    PHC2  1.010699e-69  1.4580069 0.059 0.280  3.358955e-65     other
1612                  UBE2E3  3.709143e-69  1.4103162 0.080 0.318  1.232696e-64     other
1613                  CAMTA1  5.036454e-69  1.4061603 0.079 0.315  1.673815e-64     other
1614                    ARF3  5.607618e-69  1.2883025 0.056 0.273  1.863636e-64     other
1615                    MNDA  2.032979e-68  0.2647659 0.108 0.365  6.756403e-64     other
1616                  EPSTI1  2.602547e-68  1.0176109 0.051 0.262  8.649306e-64     other
1617                  KCTD20  2.645459e-68  1.0219110 0.045 0.251  8.791918e-64     other
1618                   SNRPN  2.882467e-68  1.2616800 0.081 0.317  9.579590e-64     other
1619                    JAK3  3.382137e-68  1.1332531 0.045 0.250  1.124019e-63     other
1620                  MT-CO2  6.502081e-68 -0.2942874 0.901 0.993  2.160902e-63        mt
1621                   ABTB1  1.035500e-67  1.2339017 0.055 0.268  3.441382e-63     other
1622                    STX7  1.077773e-67  0.9653368 0.046 0.250  3.581871e-63     other
1623                  LEPROT  2.249897e-67  1.2925024 0.084 0.321  7.477308e-63     other
1624                  RIPOR2  8.826875e-67  1.7746512 0.229 0.608  2.933524e-62     other
1625                   UBE2H  1.018423e-66  1.1228385 0.062 0.278  3.384627e-62     other
1626                    NPTN  2.124169e-66  1.1290179 0.048 0.252  7.059463e-62     other
1627                  BCL2A1  2.165422e-66  1.1243835 0.067 0.286  7.196565e-62     other
1628                     CLU  1.622751e-65  2.7482475 0.331 0.208  5.393051e-61     other
1629                    MT1X  2.161141e-65  1.4157716 0.052 0.257  7.182338e-61     other
1630                    NCF1  2.395239e-65  0.8575229 0.161 0.460  7.960336e-61     other
1631                   CCPG1  4.324769e-65  1.2177099 0.055 0.261  1.437294e-60     other
1632                   RAB10  4.907325e-65  1.2288364 0.058 0.268  1.630900e-60     other
1633                   TPST2  7.316967e-65  1.1898932 0.100 0.345  2.431721e-60     other
1634                    PPIF  1.002922e-64  1.0314694 0.055 0.260  3.333112e-60     other
1635                  LGALS1  1.154861e-63  0.4551654 0.283 0.692  3.838065e-59     other
1636                     GCA  1.509710e-63  0.9856165 0.067 0.281  5.017370e-59     other
1637                    ADI1  3.763370e-63  1.1441183 0.052 0.252  1.250718e-58     other
1638                    GLUL  4.890468e-63  1.4888701 0.122 0.382  1.625298e-58     other
1639                   IRAK3  2.180885e-62  1.4561941 0.055 0.255  7.247953e-58     other
1640                   H3-3A  2.287775e-62  1.3808090 0.685 0.969  7.603193e-58     other
1641                 PLEKHO1  2.541197e-62  1.4288218 0.152 0.437  8.445413e-58     other
1642                   PPM1A  3.105482e-62  1.1931189 0.055 0.256  1.032076e-57     other
1643                    CD63  4.280331e-62  1.0607519 0.261 0.672  1.422525e-57     other
1644                  PDCD10  1.075753e-61  1.4688392 0.085 0.309  3.575157e-57     other
1645                   CEBPD  1.757465e-61  0.6536635 0.146 0.422  5.840758e-57     other
1646                   RAP1B  2.878045e-61  1.4292961 0.318 0.800  9.564895e-57     other
1647                 MT-ATP8  8.442761e-61  1.3757808 0.064 0.269  2.805867e-56        mt
1648                    OAZ1  4.433532e-60  1.6522049 0.657 0.929  1.473440e-55     other
1649                   CLIC1  9.130536e-60  0.7697138 0.314 0.793  3.034442e-55     other
1650                   CAPN1  1.842145e-59  1.6110988 0.109 0.349  6.122184e-55     other
1651                  STXBP2  4.195407e-59  1.1658769 0.174 0.476  1.394302e-54     other
1652                    NENF  6.354160e-59  1.4504631 0.068 0.271  2.111742e-54     other
1653                    RTN3  1.143347e-57  1.4997223 0.109 0.345  3.799799e-53     other
1654                     HK1  1.999984e-57  1.4539593 0.065 0.262  6.646746e-53     other
1655                 PTTG1IP  2.072868e-57  1.3579243 0.067 0.267  6.888968e-53     other
1656                  PTPN18  2.385380e-57  1.6573092 0.131 0.386  7.927573e-53     other
1657                  R3HDM4  4.972854e-57  1.4567889 0.097 0.321  1.652678e-52     other
1658                 ARHGDIB  1.252599e-56  0.4882847 0.361 0.907  4.162889e-52     other
1659                 HTATIP2  8.567402e-56  1.4192968 0.062 0.253  2.847291e-51     other
1660                    MAFB  6.797717e-55  0.4093064 0.098 0.315  2.259153e-50     other
1661                  CYB5R3  6.948895e-55  1.3313803 0.064 0.255  2.309396e-50     other
1662                   VDAC3  3.388214e-54  1.6345867 0.091 0.302  1.126039e-49     other
1663                   ASAH1  2.585297e-53  1.2723178 0.204 0.519  8.591977e-49     other
1664                    TYMP  1.907532e-52  0.2788413 0.209 0.507  6.339493e-48     other
1665                  TMSB4X  5.624646e-52  0.9963158 0.906 0.988  1.869295e-47     other
1666                   TGFB1  1.534033e-51  1.3207598 0.324 0.782  5.098206e-47     other
1667                   CSF3R  1.645207e-51  1.6908398 0.101 0.315  5.467681e-47     other
1668                SLC25A37  4.891870e-50  1.5233309 0.121 0.345  1.625764e-45     other
1669                  FCGR2A  5.018860e-50  1.1325103 0.082 0.276  1.667968e-45     other
1670                   RAB4A  2.232584e-49  1.6078846 0.083 0.276  7.419768e-45     other
1671                   RGS18  9.898500e-49  2.8405780 0.288 0.183  3.289667e-44     other
1672                    WBP2  2.788787e-48  1.6433110 0.108 0.319  9.268256e-44     other
1673                 SLC11A1  9.014991e-48  1.2243853 0.105 0.312  2.996042e-43     other
1674                 LAMTOR1  1.855985e-47  1.5500812 0.265 0.634  6.168179e-43     other
1675                  RAB11A  4.739622e-47  1.7451402 0.131 0.356  1.575166e-42     other
1676                    FPR1  1.323829e-46  1.0745142 0.101 0.303  4.399612e-42     other
1677                   PLAUR  3.445021e-45  1.3026942 0.089 0.276  1.144918e-40     other
1678                 ADIPOR1  3.562491e-45  1.6112891 0.101 0.297  1.183958e-40     other
1679                    IER3  6.665657e-44  1.2829503 0.088 0.271  2.215264e-39     other
1680                    TLK1  2.052672e-43  1.6034373 0.094 0.280  6.821851e-39     other
1681                    ACTB  2.163134e-43  0.7575137 0.846 0.984  7.188959e-39     other
1682                     MAX  6.491782e-43  1.7884385 0.147 0.376  2.157479e-38     other
1683                  CARD19  3.077983e-41  1.6843757 0.105 0.293  1.022937e-36     other
1684                   PLBD1  1.394016e-39  1.1695411 0.096 0.273  4.632874e-35     other
1685                     PKM  1.514215e-39  1.3347137 0.273 0.623  5.032341e-35     other
1686                    NRGN  1.826323e-39  2.5752931 0.408 0.366  6.069603e-35     other
1687                  ARPC1B  2.084175e-39  1.2683689 0.323 0.739  6.926547e-35     other
1688                     SNN  7.549296e-38  1.7638147 0.090 0.257  2.508933e-33     other
1689                   COTL1  9.560087e-37  0.2660346 0.324 0.684  3.177199e-32     other
1690                  SNAP23  1.521558e-36  1.8530362 0.120 0.307  5.056747e-32     other
1691                    H2AJ  2.159596e-36  1.9315301 0.171 0.402  7.177200e-32     other
1692                  TUBA1C  2.326186e-36  1.6214163 0.093 0.257  7.730847e-32     other
1693                     LAT  4.050621e-35  1.4239801 0.108 0.282  1.346183e-30     other
1694                  NAP1L1  4.253593e-34  1.7392292 0.323 0.720  1.413639e-29     other
1695                    GPX4  8.029034e-34  1.7465336 0.311 0.691  2.668369e-29     other
1696                    NCK2  4.543740e-33  1.4971852 0.113 0.283  1.510067e-28     other
1697                 NDUFAF3  1.413915e-32  1.9172202 0.122 0.299  4.699006e-28     other
1698                    CD14  1.663083e-32  0.6499859 0.136 0.321  5.527091e-28     other
1699                   NCOA4  2.564725e-32  1.8859511 0.188 0.417  8.523608e-28     other
1700                    CFL1  3.098804e-32  0.3389805 0.413 0.925  1.029856e-27     other
1701                  TALDO1  6.240702e-32  1.1732816 0.261 0.568  2.074035e-27     other
1702                    CD68  7.644759e-31  1.0240861 0.164 0.370  2.540659e-26     other
1703                   GSTO1  7.043689e-30  1.6959086 0.232 0.496  2.340900e-25     other
1704                  S100A9  7.758357e-30  1.0897332 0.592 0.561  2.578412e-25     other
1705                    IFI6  8.165092e-30  1.8175413 0.195 0.427  2.713587e-25     other
1706                   CALM3  1.783236e-29  1.7226313 0.298 0.641  5.926408e-25     other
1707                   NAMPT  2.615689e-28  1.8023412 0.211 0.447  8.692980e-24     other
1708                    TLN1  8.134652e-28  1.9820040 0.292 0.619  2.703470e-23     other
1709                   NEAT1  1.884133e-27  1.5501857 0.462 0.857  6.261728e-23     other
1710                  DYNLL1  1.823019e-26  1.8078654 0.211 0.441  6.058622e-22     other
1711                    ETFA  6.494128e-26  1.9455686 0.118 0.269  2.158259e-21     other
1712                    OST4  8.741420e-22  1.8314903 0.391 0.839  2.905124e-17     other
1713                   KIF2A  1.306043e-21  1.9776578 0.137 0.286  4.340503e-17     other
1714                 EIF2AK1  1.443802e-21  2.0818689 0.139 0.290  4.798332e-17     other
1715                     VCL  1.580608e-18  2.0357171 0.124 0.253  5.252992e-14     other
1716                    GNAS  2.168980e-18  1.3187474 0.420 0.905  7.208388e-14     other
1717                  FERMT3  3.497254e-18  1.9244791 0.263 0.507  1.162277e-13     other
1718                     ILK  1.457409e-16  2.0180452 0.140 0.270  4.843554e-12     other
1719                SH3BGRL3  2.693636e-16  0.9090512 0.665 0.956  8.952030e-12     other
1720                    STOM  1.852011e-15  1.9321893 0.161 0.302  6.154975e-11     other
1721                  IFITM3  3.539701e-15  2.1295360 0.387 0.430  1.176384e-10     other
1722                 S100A12  1.629684e-14  0.4552056 0.195 0.332  5.416093e-10     other
1723                   GAPDH  5.884242e-14  0.9059238 0.610 0.922  1.955569e-09     other
1724                    RSU1  1.776455e-12  2.1220141 0.159 0.284  5.903872e-08     other
1725                  FCER1G  1.027324e-11  0.5485384 0.308 0.529  3.414210e-07     other
1726                    TPM4  1.816600e-11  2.0001281 0.261 0.463  6.037289e-07     other
1727                    CCL5  5.662543e-11  0.3474266 0.358 0.530  1.881890e-06     other
1728                    ODC1  6.107194e-11  2.1174315 0.204 0.353  2.029665e-06     other
1729                   ACTG1  3.985807e-10  0.4825224 0.486 0.907  1.324643e-05     other
1730                  TUBA4A  1.018852e-09  2.0246905 0.263 0.455  3.386053e-05     other
1731                    SAT1  5.260914e-09  1.3764400 0.537 0.797  1.748412e-04     other
1732                    SOD2  5.995050e-05  2.0783150 0.259 0.415  1.000000e+00     other
1733                    CTSA  3.559686e-04  2.1031921 0.269 0.419  1.000000e+00     other
1734                   ITM2B  5.958429e-04  1.2821988 0.467 0.912  1.000000e+00     other
1735                   RAB32  5.991634e-04  2.2042120 0.206 0.305  1.000000e+00     other
1736                   YWHAH  6.501408e-04  2.2649492 0.214 0.317  1.000000e+00     other
1737                   HLA-E  1.012737e-03  0.4726849 0.515 0.957  1.000000e+00     other
1738                  MT-ND2  1.144595e-03  0.6024889 0.715 0.984  1.000000e+00        mt
1739                   RGS10  2.384651e-03  2.1758523 0.331 0.534  1.000000e+00     other
1740                 MARCHF2  4.238822e-03  2.2457250 0.231 0.339  1.000000e+00     other
1741                  MYL12A  5.912612e-03  1.2015419 0.474 0.901  1.000000e+00     other
1742                  TAGLN2  8.052164e-03  1.6131257 0.444 0.819  1.000000e+00     other
1743                    MYL6  1.533273e-02  0.9711086 0.538 0.936  1.000000e+00     other
1744                  NT5C3A  2.554644e-02  2.4622323 0.216 0.303  1.000000e+00     other
1745                    CST3  2.616878e-02  0.6881170 0.402 0.485  1.000000e+00     other
1746                    MPP1  5.808670e-02  2.1814328 0.188 0.251  1.000000e+00     other
1747                    SRGN  2.305441e-01  0.6434543 0.501 0.844  1.000000e+00     other
1748                   SERF2  2.782068e-01  0.8404501 0.537 0.963  1.000000e+00     other
1749                   TIMP1  5.127304e-01  2.0396879 0.346 0.498  1.000000e+00     other
1750                    IGKC  8.070496e-01  1.6080740 0.226 0.260  1.000000e+00     other
1751                   LIMS1  8.741279e-01  2.2888305 0.287 0.389  1.000000e+00     other
# Using the non-batched 'discard' vector for demonstration purposes,
# as it has more cells for stable calculation of 'lost'.
discard <- se$quality == "bad quality"
lost <- sparseMatrixStats::rowMeans2(se@assays$RNA$counts[,discard])
kept <- sparseMatrixStats::rowMeans2(se@assays$RNA$counts[,!discard])

library(edgeR)
logged <- cpm(cbind(lost, kept), log=TRUE, prior.count=2)
logFC <- logged[,1] - logged[,2]
abundance <- rowMeans(logged)

# Assuming you have a data frame with logFC and abundance
data.frame(logFC, abundance) %>%
  rownames_to_column("gene") %>%
  mutate(
    gene_type = case_when(
      str_detect(gene, pattern = "^MT-") ~ "mt",
      str_detect(gene, pattern = "^RPS|^RPL") ~ "rb",
      str_detect(gene, pattern = "^HB[^(P)]") ~ "hb",
      TRUE ~ "other"
    )
  ) %>%
  ggplot(aes(x = abundance, y = logFC, color = gene_type)) +
    geom_point() +
    geom_hline(yintercept = 1, color = "red", linetype = "dashed") +
    geom_hline(yintercept = -1, color = "red", linetype = "dashed") +
    annotate("segment", x = 12, xend = 12, y = 1, yend = 3, color = "blue", arrow = arrow(length = unit(0.2, "cm"))) +
    annotate("text", x = 13, y = 2, label = "poor quality", vjust = -1, hjust = 1, color = "blue") +
    annotate("segment", x = 12, xend = 12, y = -Inf, yend = -1, color = "blue", arrow = arrow(length = unit(0.2, "cm"), ends = "first")) +
    annotate("text", x = 12, y = -1.5, label = "good quality", vjust = 1, hjust = -0.1, color = "blue") +
    theme_classic() + 
    labs(title = "Average Gene Expression vs. Log2 Fold Change", x = "Average Gene Expression", y = "Log2 Fold Change") 

Very few genes that are differentially expressed in the low quality cells which adds a layer of confidence that we’re not removing a true celltype. This shows ribosomal-high cells as good quality cells with some hemoglbin-high as low quality.

Resave Seurat object

saveRDS(se, '../data/Covid_Flu_Seurat_Object_Quality.rds')

Session Info

sessionInfo()
R version 4.3.2 (2023-10-31)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Sonoma 14.5

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/lib/libRblas.0.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/lib/libRlapack.dylib;  LAPACK version 3.11.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: America/New_York
tzcode source: internal

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] edgeR_4.0.16         limma_3.58.1         ggridges_0.5.6       KernSmooth_2.23-22   fields_15.2          viridisLite_0.4.2    spam_2.10-0          DoubletFinder_2.0.4  colorBlindness_0.1.9 RColorBrewer_1.1-3   lubridate_1.9.3      forcats_1.0.0        stringr_1.5.1        dplyr_1.1.4          purrr_1.0.2          readr_2.1.5          tidyr_1.3.1          tibble_3.2.1         ggplot2_3.5.1        tidyverse_2.0.0      Seurat_5.0.3         SeuratObject_5.0.2   sp_2.1-4            

loaded via a namespace (and not attached):
  [1] rstudioapi_0.16.0        jsonlite_1.8.8           magrittr_2.0.3           spatstat.utils_3.0-4     farver_2.1.2             rmarkdown_2.27           vctrs_0.6.5              ROCR_1.0-11              spatstat.explore_3.2-7   htmltools_0.5.8.1        gridGraphics_0.5-1       sctransform_0.4.1        parallelly_1.37.1        htmlwidgets_1.6.4        ica_1.0-3                plyr_1.8.9               plotly_4.10.4            zoo_1.8-12               igraph_2.0.3             mime_0.12                lifecycle_1.0.4          pkgconfig_2.0.3          Matrix_1.6-5             R6_2.5.1                 fastmap_1.2.0            MatrixGenerics_1.14.0    fitdistrplus_1.1-11      future_1.33.2            shiny_1.8.1.1            digest_0.6.35            colorspace_2.1-0         patchwork_1.2.0          tensor_1.5               RSpectra_0.16-1          irlba_2.3.5.1            labeling_0.4.3           progressr_0.14.0         fansi_1.0.6              spatstat.sparse_3.0-3    timechange_0.3.0         httr_1.4.7               polyclip_1.10-6          abind_1.4-5              compiler_4.3.2           withr_3.0.0              fastDummies_1.7.3        maps_3.4.2              
 [48] MASS_7.3-60.0.1          tools_4.3.2              lmtest_0.9-40            httpuv_1.6.15            future.apply_1.11.2      goftest_1.2-3            glue_1.7.0               nlme_3.1-164             promises_1.3.0           grid_4.3.2               Rtsne_0.17               cluster_2.1.6            reshape2_1.4.4           generics_0.1.3           gtable_0.3.5             spatstat.data_3.0-4      tzdb_0.4.0               data.table_1.15.4        hms_1.1.3                utf8_1.2.4               spatstat.geom_3.2-9      RcppAnnoy_0.0.22         ggrepel_0.9.5            RANN_2.6.1               pillar_1.9.0             RcppHNSW_0.6.0           later_1.3.2              splines_4.3.2            lattice_0.22-6           survival_3.6-4           deldir_2.0-4             tidyselect_1.2.1         locfit_1.5-9.9           miniUI_0.1.1.1           pbapply_1.7-2            knitr_1.45               gridExtra_2.3            scattermore_1.2          xfun_0.44                statmod_1.5.0            matrixStats_1.3.0        stringi_1.8.4            lazyeval_0.2.2           yaml_2.3.8               evaluate_0.23            codetools_0.2-20         cli_3.6.2               
 [95] uwot_0.1.16              xtable_1.8-4             reticulate_1.36.1        munsell_0.5.1            Rcpp_1.0.12              globals_0.16.3           spatstat.random_3.2-3    png_0.1-8                parallel_4.3.2           presto_1.0.0             dotCall64_1.1-1          sparseMatrixStats_1.14.0 listenv_0.9.1            scales_1.3.0             leiden_0.4.3.1           rlang_1.1.3              cowplot_1.1.3